Chuk conserved helix-loop-helix ubiquitous kinase [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Chukprovided by MGI
Official Full Name: conserved helix-loop-helix ubiquitous kinaseprovided by MGI
Primary source: MGI:MGI:99484
See related: Ensembl:ENSMUSG00000025199 AllianceGenome:MGI:99484
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: IKK1; Ikka; Chuk1; Fbx24; IKBKA; Fbxo24; NFKBIKA; IKK alpha
Summary: Enables IkappaB kinase activity. Involved in several processes, including integrin-mediated signaling pathway; positive regulation of interferon-alpha production; and response to virus. Acts upstream of or within several processes, including mammary gland development; osteoclast differentiation; and response to muscle stretch. Located in cytoplasmic side of plasma membrane. Part of CD40 receptor complex and IkappaB kinase complex. Is expressed in several structures, including alimentary system; central nervous system; genitourinary system; hemolymphoid system gland; and limb. Used to study fetal encasement syndrome. Human ortholog(s) of this gene implicated in fetal encasement syndrome; liver cancer; and prostate cancer. Orthologous to human CHUK (component of inhibitor of nuclear factor kappa B kinase complex). [provided by Alliance of Genome Resources, Oct 2024]
Expression: Ubiquitous expression in CNS E18 (RPKM 12.2), CNS E14 (RPKM 11.6) and 28 other tissues See more
Orthologs: human all
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Genomic context

Location:: 19 C3; 19 36.71 cM

Exon count:: 21

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	19	NC_000085.7 (44061773..44095940, complement)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	19	NC_000085.6 (44073334..44107505, complement)

Chromosome 19 - NC_000085.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

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Expression

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See details

Project title: Mouse ENCODE transcriptome data Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Loss of Lymphatic IKKalpha Disrupts Lung Immune Homeostasis, Drives BALT Formation, and Protects against Influenza.
Title: Loss of Lymphatic IKKα Disrupts Lung Immune Homeostasis, Drives BALT Formation, and Protects against Influenza.
IKK1 aggravates ischemia-reperfusion kidney injury by promoting the differentiation of effector T cells.
Title: IKK1 aggravates ischemia-reperfusion kidney injury by promoting the differentiation of effector T cells.
Conditional loss of IKKalpha in Osterix + cells has no effect on bone but leads to age-related loss of peripheral fat.
Title: Conditional loss of IKKα in Osterix + cells has no effect on bone but leads to age-related loss of peripheral fat.
Deficiency of IKKalpha in Macrophages Mitigates Fibrosis Progression in the Kidney after Renal Ischemia-Reperfusion Injury.
Title: Deficiency of IKKα in Macrophages Mitigates Fibrosis Progression in the Kidney after Renal Ischemia-Reperfusion Injury.
IKKalpha mediates UVB-induced cell apoptosis by regulating p53 pathway activation.
Title: IKKα mediates UVB-induced cell apoptosis by regulating p53 pathway activation.
Deletion of IKK2 in haematopoietic cells of adult mice leads to elevated interleukin-6, neutrophilia and fatal gastrointestinal inflammation.
Title: Deletion of IKK2 in haematopoietic cells of adult mice leads to elevated interleukin-6, neutrophilia and fatal gastrointestinal inflammation.
Inducible knockout of CHUK/IKKalpha in adult chondrocytes reduces progression of cartilage degradation in a surgical model of osteoarthritis.
Title: Inducible knockout of CHUK/IKKα in adult chondrocytes reduces progression of cartilage degradation in a surgical model of osteoarthritis.
The data show that IKKa and IKKb regulate unique genes in pulmonary endothelial cells, resulting in differential effects on lung angiogenesis.
Title: Distinct roles for IκB kinases alpha and beta in regulating pulmonary endothelial angiogenic function during late lung development.
our data reveals that IKKa is a novel mediator protecting against the development of myocardial I/R injury via negative regulation of macrophage polarization to M1 phenotype.
Title: Regulatory role of IKKɑ in myocardial ischemia/reperfusion injury by the determination of M1 versus M2 polarization of macrophages.
Data suggest a direct link between microtubule-associated protein 1-light chain 3 (LC3)-associated phagocytosis (LAP) formation and IKK alpha (IKKalpha) recruitment downstream of -like receptor 9 (TLR9) activation that is necessary to facilitate type I IFN production.
Title: The interaction between IKKα and LC3 promotes type I interferon production through the TLR9-containing LAPosome.

Submit: New GeneRIF Correction See all GeneRIFs (133)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Endonuclease-mediated (5)
Targeted (9) 1 citation

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

Chuk (e-PCR), detects polymorphism
- UniSTS:141340

Chuk (e-PCR), detects polymorphism
- UniSTS:141341

RH136061 (e-PCR)
- UniSTS:210056

U12473 (e-PCR)
- UniSTS:125671

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables IkappaB kinase activity	IDA Inferred from Direct Assay more info	PubMed
enables IkappaB kinase activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables IkappaB kinase activity	ISO Inferred from Sequence Orthology more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein heterodimerization activity	ISO Inferred from Sequence Orthology more info
enables protein heterodimerization activity	ISS Inferred from Sequence or Structural Similarity more info
enables protein homodimerization activity	ISO Inferred from Sequence Orthology more info
enables protein homodimerization activity	ISS Inferred from Sequence or Structural Similarity more info
enables protein kinase activity	IDA Inferred from Direct Assay more info	PubMed
enables protein kinase activity	ISO Inferred from Sequence Orthology more info
enables protein kinase activity	ISS Inferred from Sequence or Structural Similarity more info
enables protein serine/threonine kinase activity	IBA Inferred from Biological aspect of Ancestor more info
enables protein serine/threonine kinase activity	ISO Inferred from Sequence Orthology more info
enables protein serine/threonine kinase activity	ISS Inferred from Sequence or Structural Similarity more info
enables protein-containing complex binding	ISO Inferred from Sequence Orthology more info
enables scaffold protein binding	ISO Inferred from Sequence Orthology more info	PubMed
enables transferrin receptor binding	ISO Inferred from Sequence Orthology more info

Process	Evidence Code	Pubs
involved_in I-kappaB phosphorylation	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within I-kappaB phosphorylation	TAS Traceable Author Statement more info	PubMed
involved_in canonical NF-kappaB signal transduction	IDA Inferred from Direct Assay more info	PubMed
involved_in canonical NF-kappaB signal transduction	IGI Inferred from Genetic Interaction more info	PubMed
involved_in canonical NF-kappaB signal transduction	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in canonical NF-kappaB signal transduction	ISO Inferred from Sequence Orthology more info
involved_in cellular response to cadmium ion	ISO Inferred from Sequence Orthology more info
involved_in cellular response to reactive oxygen species	ISO Inferred from Sequence Orthology more info
involved_in cellular response to tumor necrosis factor	ISO Inferred from Sequence Orthology more info
involved_in cellular response to tumor necrosis factor	ISS Inferred from Sequence or Structural Similarity more info
involved_in cellular response to virus	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in cellular response to virus	ISO Inferred from Sequence Orthology more info
involved_in defense response to virus	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in integrin-mediated signaling pathway	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within lactation	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within lactation	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within mammary gland alveolus development	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within mammary gland alveolus development	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within mammary gland epithelial cell proliferation	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within mammary gland epithelial cell proliferation	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within morphogenesis of an epithelial sheet	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of NF-kappaB transcription factor activity	ISO Inferred from Sequence Orthology more info
involved_in non-canonical NF-kappaB signal transduction	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within odontogenesis of dentin-containing tooth	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within osteoclast differentiation	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within osteoclast differentiation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of DNA-templated transcription	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of canonical NF-kappaB signal transduction	IBA Inferred from Biological aspect of Ancestor more info
involved_in positive regulation of canonical NF-kappaB signal transduction	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of interferon-alpha production	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of interferon-alpha production	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of transcription by RNA polymerase II	IBA Inferred from Biological aspect of Ancestor more info
involved_in positive regulation of transcription by RNA polymerase II	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of transcription by RNA polymerase II	ISS Inferred from Sequence or Structural Similarity more info
involved_in protein phosphorylation	ISO Inferred from Sequence Orthology more info
involved_in response to cholecystokinin	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within response to muscle stretch	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in striated muscle cell differentiation	ISO Inferred from Sequence Orthology more info
involved_in toll-like receptor 4 signaling pathway	ISO Inferred from Sequence Orthology more info
involved_in tumor necrosis factor-mediated signaling pathway	IBA Inferred from Biological aspect of Ancestor more info
involved_in tumor necrosis factor-mediated signaling pathway	ISO Inferred from Sequence Orthology more info
involved_in tumor necrosis factor-mediated signaling pathway	TAS Traceable Author Statement more info	PubMed

Component	Evidence Code	Pubs
part_of CD40 receptor complex	IDA Inferred from Direct Assay more info	PubMed
part_of IkappaB kinase complex	IBA Inferred from Biological aspect of Ancestor more info
part_of IkappaB kinase complex	IDA Inferred from Direct Assay more info	PubMed
part_of IkappaB kinase complex	IPI Inferred from Physical Interaction more info	PubMed
part_of IkappaB kinase complex	ISO Inferred from Sequence Orthology more info
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
located_in cytoplasm	ISO Inferred from Sequence Orthology more info
located_in cytoplasm	TAS Traceable Author Statement more info	PubMed
located_in cytoplasmic side of plasma membrane	IDA Inferred from Direct Assay more info	PubMed
located_in cytosol	ISO Inferred from Sequence Orthology more info
located_in nucleoplasm	ISO Inferred from Sequence Orthology more info
located_in nucleus	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: inhibitor of nuclear factor kappa-B kinase subunit alpha

Names: I kappa B kinase 1; I-kappa-B kinase alpha; IkappaB kinase alpha; nuclear factor NF-kappa-B inhibitor kinase alpha

NP_001155882.1

EC 2.7.11.10

NP_031726.2

EC 2.7.11.10

XP_030106592.1

EC 2.7.11.10

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001162410.1 → NP_001155882.1 inhibitor of nuclear factor kappa-B kinase subunit alpha isoform 2

Status: VALIDATED

Description

Transcript Variant: This variant (2) uses an alternate splice site in the 3' coding region compared to variant 1, that results in a frameshift. The encoded isoform (2) is shorter and has a distinct C-terminus compared to isoform 1.

Source sequence(s)

AC124693, AK035326, AK164236

Consensus CDS

CCDS50444.1

UniProtKB/TrEMBL

E9Q605, Q3TPN9, Q3U2A3

Related

ENSMUSP00000113809.2, ENSMUST00000119591.2

Conserved Domains (2) summary

smart00220
Location:15 → 298

S_TKc; Serine/Threonine protein kinases, catalytic domain

cd14039
Location:21 → 309

STKc_IKK_alpha; Catalytic domain of the Serine/Threonine kinase, Inhibitor of Nuclear Factor-KappaB Kinase (IKK) alpha
NM_007700.2 → NP_031726.2 inhibitor of nuclear factor kappa-B kinase subunit alpha isoform 1

Status: VALIDATED

Description

Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).

Source sequence(s)

AC124693, AK035326, AK150039, AK164236

Consensus CDS

CCDS29843.1

UniProtKB/Swiss-Prot

Q60680, Q80VU2, Q9D2X3

UniProtKB/TrEMBL

E9QNL4, Q8CBT3

Related

ENSMUSP00000026217.5, ENSMUST00000026217.11

Conserved Domains (3) summary

smart00220
Location:15 → 298

S_TKc; Serine/Threonine protein kinases, catalytic domain

cd14039
Location:21 → 309

STKc_IKK_alpha; Catalytic domain of the Serine/Threonine kinase, Inhibitor of Nuclear Factor-KappaB Kinase (IKK) alpha

pfam12179
Location:709 → 743

IKKbetaNEMObind; I-kappa-kinase-beta NEMO binding domain

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000085.7 Reference GRCm39 C57BL/6J

Range

44061773..44095940 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_030250732.2 → XP_030106592.1 inhibitor of nuclear factor kappa-B kinase subunit alpha isoform X1

UniProtKB/TrEMBL

Q3TPN9, Q3U2A3

Conserved Domains (3) summary

cd14039
Location:21 → 309

STKc_IKK_alpha; Catalytic domain of the Serine/Threonine kinase, Inhibitor of Nuclear Factor-KappaB Kinase (IKK) alpha

cd17046
Location:311 → 387

Ubl_IKKA_like; ubiquitin-like (Ubl) domain found in inhibitor of nuclear factor kappa-B kinases, IKK-alpha and IKK-beta, and similar proteins

pfam18397
Location:386 → 659

IKBKB_SDD; IQBAL scaffold dimerization domain