FHL3 four and a half LIM domains 3 [Homo sapiens (human)] - Gene

Summary

Official Symbol: FHL3provided by HGNC
Official Full Name: four and a half LIM domains 3provided by HGNC
Primary source: HGNC:HGNC:3704
See related: Ensembl:ENSG00000183386 MIM:602790; AllianceGenome:HGNC:3704
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Homo sapiens
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
Also known as: SLIM2
Summary: The protein encoded by this gene is a member of a family of proteins containing a four-and-a-half LIM domain, which is a highly conserved double zinc finger motif. The encoded protein has been shown to interact with the cancer developmental regulators SMAD2, SMAD3, and SMAD4, the skeletal muscle myogenesis protein MyoD, and the high-affinity IgE beta chain regulator MZF-1. This protein may be involved in tumor suppression, repression of MyoD expression, and repression of IgE receptor expression. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2011]
Expression: Ubiquitous expression in esophagus (RPKM 21.3), endometrium (RPKM 16.8) and 25 other tissues See more
Orthologs: mouse all
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Genomic context

Location:: 1p34.3

Exon count:: 6

Annotation release	Status	Assembly	Chr	Location
RS_2024_08	current	GRCh38.p14 (GCF_000001405.40)	1	NC_000001.11 (37996770..38005514, complement)
RS_2024_08	current	T2T-CHM13v2.0 (GCF_009914755.1)	1	NC_060925.1 (37863951..37872693, complement)
RS_2024_09	previous assembly	GRCh37.p13 (GCF_000001405.25)	1	NC_000001.10 (38462442..38471186, complement)

Chromosome 1 - NC_000001.11 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: HPA RNA-seq normal tissues
Description: RNA-seq was performed of tissue samples from 95 human individuals representing 27 different tissues in order to determine tissue-specificity of all protein-coding genes
BioProject: PRJEB4337
Publication: PMID 24309898
Analysis date: Wed Apr 4 07:08:55 2018

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Upregulation of FHL1, SPNS3, and MPZL2 predicts poor prognosis in pediatric acute myeloid leukemia patients with FLT3-ITD mutation.
Title: Upregulation of FHL1, SPNS3, and MPZL2 predicts poor prognosis in pediatric acute myeloid leukemia patients with FLT3-ITD mutation.
PCBP2 promotes the development of glioma by regulating FHL3/TGF-beta/Smad signaling pathway.
Title: PCBP2 promotes the development of glioma by regulating FHL3/TGF-β/Smad signaling pathway.
these findings highlight the role of FHL3 as a stemness-suppressor in regulation of the Smad2/3-SOX4-SOX2 axis in glioma
Title: FHL3 links cell growth and self-renewal by modulating SOX4 in glioma.
the present study established a link between Ang and FHL3 proteins and identifies a new pathway for regulating astrocytoma progression.
Title: Angiogenin promotes U87MG cell proliferation by activating NF-κB signaling pathway and downregulating its binding partner FHL3.
These findings suggest that functional interactions between FHL3 and MT-1X may provide some clues to the mechanisms of FHL3-regulated cell proliferation.
Title: Identification and characterization of MT-1X as a novel FHL3-binding partner.
PCBP2 could bind to FHL3 mRNA 3'UTR-A and inhibited the expression of FHL3 in T98G glioms cells
Title: Ribotrap analysis of proteins associated with FHL3 3'untranslated region in glioma cells.
FHL3 enhances Tax1-mediated activation of the Human T-cell leukemia virus type 1 long terminal repeat (LTR) without affecting basal activity and regulates NF-kappaB activation by Tax1 in a cell-specific manner.
Title: The four and a half LIM family members are novel interactants of the human T-cell leukemia virus type 1 Tax oncoprotein.
findings suggest that the interaction between Ang and FHL3 may provide some clues to the mechanisms of Ang-regulated cell growth and apoptosis
Title: Identification and characterization of FHL3 as a novel angiogenin-binding partner.
FHL3 suppressed anchorage-dependent and -independent growth of human breast cancer cells.
Title: Downregulation and antiproliferative role of FHL3 in breast cancer.
FHL3 does not bind to HIF-1alpha or p300, indicating that it regulates transactivation by a novel molecular mechanism.
Title: Four-and-a-half LIM domain proteins inhibit transactivation by hypoxia-inducible factor 1.

Submit: New GeneRIF Correction See all GeneRIFs (16)

Phenotypes

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Find tests for this gene in the NIH Genetic Testing Registry (GTR)

Review eQTL and phenotype association data in this region using PheGenI

Variation

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See variants in ClinVar

See studies and variants in dbVar

See Variation Viewer (GRCh37.p13)

See Variation Viewer (GRCh38)

HIV-1 interactions

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Replication interactions

Interaction	Pubs
Knockdown of four and a half LIM domains 3 (FHL3) by siRNA inhibits HIV-1 replication in HeLa-derived TZM-bl cells	PubMed

Go to the HIV-1, Human Interaction Database

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

D1S2017E (e-PCR)
- UniSTS:151382

FHL3_1356 (e-PCR)
- UniSTS:277239

SHGC-74628 (e-PCR)
- UniSTS:72952

Gene Ontology Provided by GOA

Function	Evidence Code	Pubs
enables actin binding	IBA Inferred from Biological aspect of Ancestor more info
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables transcription coregulator activity	IBA Inferred from Biological aspect of Ancestor more info

Process	Evidence Code	Pubs
involved_in actin cytoskeleton organization	IBA Inferred from Biological aspect of Ancestor more info
involved_in muscle organ development	TAS Traceable Author Statement more info	PubMed

Component	Evidence Code	Pubs
is_active_in Z disc	IBA Inferred from Biological aspect of Ancestor more info
located_in focal adhesion	HDA more info	PubMed
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
is_active_in stress fiber	IBA Inferred from Biological aspect of Ancestor more info

General protein information

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Preferred Names: four and a half LIM domains protein 3

Names: FHL-3; LIM-only protein FHL3; SLIM-2; skeletal muscle LIM-protein 2

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001243878.2 → NP_001230807.1 four and a half LIM domains protein 3 isoform 2

See identical proteins and their annotated locations for NP_001230807.1

Status: REVIEWED

Description

Transcript Variant: This variant (2) differs in the 5' UTR and coding sequence compared to variant 1. The resulting isoform (2) is shorter at the N-terminus compared to isoform 1.

Source sequence(s)

AL603790

UniProtKB/TrEMBL

Q96C98

Related

ENST00000485803.5

Conserved Domains (3) summary

cd09346
Location:54 → 105

LIM3_FHL; The third LIM domain of Four and a half LIM domains protein (FHL)

cd09434
Location:113 → 168

LIM4_FHL3; The fourth LIM domain of Four and a half LIM domains protein 3 (FHL3)

cl02475
Location:1 → 47

LIM; LIM is a small protein-protein interaction domain, containing two zinc fingers
NM_004468.5 → NP_004459.2 four and a half LIM domains protein 3 isoform 1

See identical proteins and their annotated locations for NP_004459.2

Status: REVIEWED

Description

Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).

Source sequence(s)

AL603790

Consensus CDS

CCDS30678.1

UniProtKB/Swiss-Prot

D3DPT6, Q13643, Q6I9T0, Q9BVA2

UniProtKB/TrEMBL

Q9P100

Related

ENSP00000362107.3, ENST00000373016.4

Conserved Domains (5) summary

cd09346
Location:162 → 213

LIM3_FHL; The third LIM domain of Four and a half LIM domains protein (FHL)

cd09423
Location:36 → 94

LIM1_FHL3; The first LIM domain of Four and a half LIM domains protein 3 (FHL3)

cd09427
Location:98 → 155

LIM2_FHL3; The second LIM domain of Four and a half LIM domains protein 3 (FHL3)

cd09434
Location:221 → 276

LIM4_FHL3; The fourth LIM domain of Four and a half LIM domains protein 3 (FHL3)

cl02475
Location:5 → 33

LIM; LIM is a small protein-protein interaction domain, containing two zinc fingers