RPB8 DNA-directed RNA polymerase core subunit RPB8 [Saccharomyces cerevisiae S288C] - Gene

Summary

Go to the top of the page Help

Official Symbol: RPB8
Official Full Name: DNA-directed RNA polymerase core subunit RPB8
Primary source: SGD:S000005750
Locus tag: YOR224C
See related: AllianceGenome:SGD:S000005750; FungiDB:YOR224C; VEuPathDB:YOR224C
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary: Contributes to 5'-3' RNA polymerase activity. Involved in tRNA transcription by RNA polymerase III; transcription by RNA polymerase I; and transcription by RNA polymerase II. Part of RNA polymerase I complex; RNA polymerase II, core complex; and RNA polymerase III complex. Orthologous to human POLR2H (RNA polymerase II, I and III subunit H). [provided by Alliance of Genome Resources, Apr 2022]
NEW: Try the new Gene table
Try the new Transcript table

Genomic context

Go to the top of the page Help

See RPB8 in Genome Data Viewer

Location:: chromosome: XV

Exon count:: 1

Sequence:: Chromosome: XV; NC_001147.6 (760825..761265, complement)

Chromosome XV - NC_001147.6 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

Go to the top of the page Help

Go to reference sequence details

Genomic Sequence:: NC_001147.6

Go to nucleotide: Graphics FASTA GenBank

Bibliography

Go to the top of the page Help

GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Data show that Top2 is required redundantly with Top1 for optimal recruitment of RNA polymerase II at their promoters.
Title: Topoisomerase II binds nucleosome-free DNA and acts redundantly with topoisomerase I to enhance recruitment of RNA Pol II in budding yeast.

Submit: New GeneRIF Correction

Pathways from PubChem

Go to the top of the page

Interactions

Go to the top of the page Help

Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

Go to the top of the page Help

Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables DNA binding	IEA Inferred from Electronic Annotation more info
enables DNA-directed 5'-3' RNA polymerase activity	IEA Inferred from Electronic Annotation more info
contributes_to RNA polymerase I activity	IBA Inferred from Biological aspect of Ancestor more info
contributes_to RNA polymerase I activity	IDA Inferred from Direct Assay more info	PubMed
contributes_to RNA polymerase II activity	IBA Inferred from Biological aspect of Ancestor more info
contributes_to RNA polymerase II activity	IDA Inferred from Direct Assay more info	PubMed
contributes_to RNA polymerase III activity	IBA Inferred from Biological aspect of Ancestor more info
contributes_to RNA polymerase III activity	IDA Inferred from Direct Assay more info	PubMed
contributes_to RNA-dependent RNA polymerase activity	IDA Inferred from Direct Assay more info	PubMed

Process	Evidence Code	Pubs
involved_in DNA-templated transcription	IEA Inferred from Electronic Annotation more info
involved_in RNA-templated transcription	IEA Inferred from Electronic Annotation more info
involved_in ribosome biogenesis	IEA Inferred from Electronic Annotation more info
involved_in tRNA transcription by RNA polymerase III	IDA Inferred from Direct Assay more info	PubMed
involved_in transcription by RNA polymerase I	IDA Inferred from Direct Assay more info	PubMed
involved_in transcription by RNA polymerase I	IPI Inferred from Physical Interaction more info	PubMed
involved_in transcription by RNA polymerase II	IPI Inferred from Physical Interaction more info	PubMed
involved_in transcription by RNA polymerase III	IGI Inferred from Genetic Interaction more info	PubMed
involved_in transcription by RNA polymerase III	IPI Inferred from Physical Interaction more info	PubMed

Component	Evidence Code	Pubs
part_of DNA-directed RNA polymerase complex	IEA Inferred from Electronic Annotation more info
part_of RNA polymerase I complex	IBA Inferred from Biological aspect of Ancestor more info
part_of RNA polymerase I complex	IDA Inferred from Direct Assay more info	PubMed
part_of RNA polymerase II, core complex	IBA Inferred from Biological aspect of Ancestor more info
part_of RNA polymerase II, core complex	IDA Inferred from Direct Assay more info	PubMed
part_of RNA polymerase III complex	IBA Inferred from Biological aspect of Ancestor more info
part_of RNA polymerase III complex	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	IEA Inferred from Electronic Annotation more info

General protein information

Go to the top of the page Help

Preferred Names: DNA-directed RNA polymerase core subunit RPB8

NP_014867.1

RNA polymerase subunit ABC14.5; common to RNA polymerases I, II, and III

NCBI Reference Sequences (RefSeq)

Go to the top of the page Help

NEW Try the new Transcript table

Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001147.6 Reference assembly

Range

760825..761265 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001183643.1 → NP_014867.1 TPA: DNA-directed RNA polymerase core subunit RPB8 [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_014867.1

Status: REVIEWED

UniProtKB/Swiss-Prot

D6W2S9, P20436

UniProtKB/TrEMBL

A6ZP66, B3LJP7, C7GWF9, C8ZGX8, G2WN95, N1NVW0

Conserved Domains (1) summary

pfam03870
Location:10 → 145

RNA_pol_Rpb8; RNA polymerase Rpb8