MSB2 [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: MSB2
Primary source: SGD:S000003246
Locus tag: YGR014W
See related: AllianceGenome:SGD:S000003246; FungiDB:YGR014W; VEuPathDB:YGR014W
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary: Enables osmosensor activity. Involved in several processes, including hyperosmotic response; osmosensory signaling pathway via Sho1 osmosensor; and signal transduction involved in filamentous growth. Located in extracellular region and site of polarized growth. Is integral component of plasma membrane. [provided by Alliance of Genome Resources, Apr 2022]
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Genomic context

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Location:: chromosome: VII

Exon count:: 1

Sequence:: Chromosome: VII; NC_001139.9 (516943..520863)

Chromosome VII - NC_001139.9 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001139.9

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

A subunit of actin capping protein, Cap2p, interacted with the cytoplasmic domain of Msb2p.
Title: Proteins That Interact with the Mucin-Type Glycoprotein Msb2p Include a Regulator of the Actin Cytoskeleton.
Msb2 is required for pressure sensing. Msb2 is linked to the actin cortex.
Title: SCWISh network is essential for survival under mechanical pressure.
The extracellular cysteine-rich domain of the transmembrane-anchor protein Opy2 binds to the Hkr1-Msb2 homology domain of the putative osmosensor Msb2 and the formation of the Opy2-Msb2 complex is essential for osmotic activation of Hog1.
Title: Binding of the Extracellular Eight-Cysteine Motif of Opy2 to the Putative Osmosensor Msb2 Is Essential for Activation of the Yeast High-Osmolarity Glycerol Pathway.
Our results confirm the existence of negative feedback systems targeting the protein levels of Ste11 and Msb2 and also hint at changes in the dissociation rates of intermediate signaling complexes
Title: Msb2 is a Ste11 membrane concentrator required for full activation of the HOG pathway.
interaction between Ste20 and Sho1 contributed to the activation of Hog1 by Hkr1, but not by Msb2.
Title: Yeast osmosensors Hkr1 and Msb2 activate the Hog1 MAPK cascade by different mechanisms.
The osmosensor Hkr1 is required for activation of the yeast cell wall integrity MAPK pathway signaling but Msb2 is not.
Title: Activation of the yeast cell wall integrity MAPK pathway by zymolyase depends on protease and glucanase activities and requires the mucin-like protein Hkr1 but not Msb2.
Data showed that Msb2p had a function in cell polarization through the adaptor protein Sho1p that Hkr1p did not.
Title: The signaling mucins Msb2 and Hkr1 differentially regulate the filamentation mitogen-activated protein kinase pathway and contribute to a multimodal response.
Saccharomyces cerevisiae mucin Msb2p is processed into secreted and cell-associated forms, by the aspartyl protease Yps1p.
Title: Cleavage of the signaling mucin Msb2 by the aspartyl protease Yps1 is required for MAPK activation in yeast.
Hkr1 and Msb2 individually form a complex with Sho1, and, upon high external osmolarity stress, appear to induce Sho1 to generate an intracellular signal
Title: Transmembrane mucins Hkr1 and Msb2 are putative osmosensors in the SHO1 branch of yeast HOG pathway.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables osmosensor activity	IBA Inferred from Biological aspect of Ancestor more info
enables osmosensor activity	IEA Inferred from Electronic Annotation more info
enables osmosensor activity	IMP Inferred from Mutant Phenotype more info	PubMed

Process	Evidence Code	Pubs
involved_in cellular bud site selection	IBA Inferred from Biological aspect of Ancestor more info
involved_in establishment of cell polarity	IGI Inferred from Genetic Interaction more info	PubMed
involved_in establishment of cell polarity	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in fungal-type cell wall organization	IBA Inferred from Biological aspect of Ancestor more info
involved_in hyperosmotic response	IBA Inferred from Biological aspect of Ancestor more info
involved_in hyperosmotic response	IGI Inferred from Genetic Interaction more info	PubMed
involved_in osmosensory signaling pathway via Sho1 osmosensor	IBA Inferred from Biological aspect of Ancestor more info
involved_in osmosensory signaling pathway via Sho1 osmosensor	IEA Inferred from Electronic Annotation more info
involved_in osmosensory signaling pathway via Sho1 osmosensor	IGI Inferred from Genetic Interaction more info	PubMed
involved_in response to osmotic stress	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in signal transduction involved in filamentous growth	IBA Inferred from Biological aspect of Ancestor more info
involved_in signal transduction involved in filamentous growth	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
is_active_in cell surface	IBA Inferred from Biological aspect of Ancestor more info
is_active_in extracellular region	IBA Inferred from Biological aspect of Ancestor more info
located_in extracellular region	IDA Inferred from Direct Assay more info	PubMed
is_active_in plasma membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in plasma membrane	IEA Inferred from Electronic Annotation more info
located_in plasma membrane	IMP Inferred from Mutant Phenotype more info	PubMed
is_active_in site of polarized growth	IBA Inferred from Biological aspect of Ancestor more info
located_in site of polarized growth	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: Msb2p

NP_011528.3

Mucin family member involved in various signaling pathways; functions as osmosensor in the Sho1p-mediated HOG pathway; functions in Cdc42p- and MAP kinase-dependent filamentous growth signaling pathway; processed into secreted and cell-associated forms by aspartyl protease Yps1p; potential Cdc28p substrate