Rnf7 ring finger protein 7 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Rnf7provided by MGI
Official Full Name: ring finger protein 7provided by MGI
Primary source: MGI:MGI:1337096
See related: Ensembl:ENSMUSG00000051234 AllianceGenome:MGI:1337096
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: SAG; Rbx2
Summary: Enables ubiquitin protein ligase activity. Involved in proteasome-mediated ubiquitin-dependent protein catabolic process and regulation of neuron migration. Acts upstream of with a negative effect on reelin-mediated signaling pathway. Acts upstream of or within several processes, including brain development; intrinsic apoptotic signaling pathway in response to oxidative stress; and positive regulation of cysteine-type endopeptidase activity. Located in cytoplasm and nucleus. Part of Cul5-RING ubiquitin ligase complex. Is expressed in central nervous system; eye; and genitourinary system. Orthologous to human RNF7 (ring finger protein 7). [provided by Alliance of Genome Resources, Dec 2024]
Expression: Ubiquitous expression in CNS E11.5 (RPKM 73.0), CNS E14 (RPKM 56.6) and 28 other tissues See more
Orthologs: human all
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Genomic context

Location:: 9 E3.3; 9 50.63 cM

Exon count:: 3

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	9	NC_000075.7 (96352990..96360728, complement)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	9	NC_000075.6 (96470937..96478675, complement)

Chromosome 9 - NC_000075.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

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Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Ubiquitin Ligase RBX2/SAG Regulates Mitochondrial Ubiquitination and Mitophagy.
Title: Ubiquitin Ligase RBX2/SAG Regulates Mitochondrial Ubiquitination and Mitophagy.
Sag/Rbx2 Partial Inactivation Sensitizes Mice to Radiation and Radiation-Induced Tumorigenesis1.
Title: Sag/Rbx2 Partial Inactivation Sensitizes Mice to Radiation and Radiation-Induced Tumorigenesis1.
The Sag-Shoc2 axis regulates conversion of mPanINs to cystic lesions in Kras pancreatic tumor model.
Title: The Sag-Shoc2 axis regulates conversion of mPanINs to cystic lesions in Kras pancreatic tumor model.
Study in transgenic mice with selective Sag deletion in myeloid lineage found that in contrast to in vitro observations those mice showed increased serum levels of proinflammatory cytokines and enhanced mortality in response to LPS. Sag-/- macrophages released less proinflammatory cytokines, neutrophils released more. Study revealed that Sag plays a differential role in the activation of macrophages and neutrophils.
Title: SAG/RBX2 E3 Ubiquitin Ligase Differentially Regulates Inflammatory Responses of Myeloid Cell Subsets.
In the brain, Cul5 and Rbx2 are expressed in a similar fashion
Title: Comparative Analysis of cul5 and rbx2 Expression in the Developing and Adult Murine Brain and Their Essentiality During Mouse Embryogenesis.
RBX2, a core component of the E3 ubiquitin ligase CRL5, is essential for retinal layering and function. RBX2 regulates the final cell position of rod bipolar cells, cone photoreceptors and Muller glia. Our data indicate that sustained RELN/DAB1 signaling, triggered by depletion of RBX2 or SOCS7 - a CRL5 substrate adaptor known to recruit DAB1 - causes rod bipolar cell misposition.
Title: RBX2 maintains final retinal cell position in a DAB1-dependent and -independent fashion.
SAG is a novel molecular target that regulates T-cell responses and that inhibiting neddylation with the clinically available small-molecule MLN4924 may represent a novel strategy to mitigate T-cell-mediated immunopathologies, such as graft-versus-host disease.
Title: SAG/Rbx2-Dependent Neddylation Regulates T-Cell Responses.
Attenuation of SAG expression, exacerbates 4-hydroxy-2-nonenal-induced apoptosis and hypertrophy via a disruption of the cellular redox balance.
Title: Attenuated SAG expression exacerbates 4-hydroxy-2-nonenal-induced apoptosis and hypertrophy of H9c2 cardiomyocytes.
Inactivation of Sag/Rbx2/Roc2 e3 ubiquitin ligase triggers senescence and inhibits kras-induced immortalization.
Title: Inactivation of Sag/Rbx2/Roc2 e3 ubiquitin ligase triggers senescence and inhibits kras-induced immortalization.
as a novel substrate of SAG-betaTrCP E3 ligase. By degrading Erbin and Nrf2, Sag activates the Ras-Raf pathway and causes ROS accumulation to trigger autophagy and senescence
Title: Erbin is a novel substrate of the Sag-βTrCP E3 ligase that regulates KrasG12D-induced skin tumorigenesis.

Submit: New GeneRIF Correction See all GeneRIFs (14)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Gene trapped (2)
Targeted (3) 1 citation

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables NEDD8 ligase activity	ISO Inferred from Sequence Orthology more info
enables NEDD8 ligase activity	ISS Inferred from Sequence or Structural Similarity more info
enables cullin family protein binding	IBA Inferred from Biological aspect of Ancestor more info
enables cullin family protein binding	ISO Inferred from Sequence Orthology more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables ubiquitin protein ligase activity	IBA Inferred from Biological aspect of Ancestor more info
enables ubiquitin protein ligase activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables zinc ion binding	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
acts_upstream_of_or_within apoptotic mitochondrial changes	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within cerebral cortex radially oriented cell migration	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within cerebral cortex radially oriented cell migration	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within intrinsic apoptotic signaling pathway in response to oxidative stress	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within negative regulation of apoptotic process	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within positive regulation of cysteine-type endopeptidase activity	IDA Inferred from Direct Assay more info	PubMed
involved_in proteasome-mediated ubiquitin-dependent protein catabolic process	IDA Inferred from Direct Assay more info	PubMed
involved_in protein neddylation	ISO Inferred from Sequence Orthology more info
involved_in protein neddylation	ISS Inferred from Sequence or Structural Similarity more info
involved_in protein ubiquitination	IBA Inferred from Biological aspect of Ancestor more info
involved_in protein ubiquitination	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within radial glia guided migration of Purkinje cell	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within radial glia guided migration of Purkinje cell	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_negative_effect reelin-mediated signaling pathway	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of neuron migration	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in ubiquitin-dependent protein catabolic process	IBA Inferred from Biological aspect of Ancestor more info

Component	Evidence Code	Pubs
part_of Cul5-RING ubiquitin ligase complex	IBA Inferred from Biological aspect of Ancestor more info
part_of Cul5-RING ubiquitin ligase complex	IDA Inferred from Direct Assay more info	PubMed
part_of Cul5-RING ubiquitin ligase complex	ISO Inferred from Sequence Orthology more info
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
located_in cytosol	ISO Inferred from Sequence Orthology more info
located_in cytosol	TAS Traceable Author Statement more info
located_in nucleoplasm	ISO Inferred from Sequence Orthology more info
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: RING-box protein 2

Names: sensitive to apoptosis gene protein

NP_001298064.1

EC 2.3.2.27

EC 2.3.2.32

NP_035409.1

EC 2.3.2.27

EC 2.3.2.32

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001311135.1 → NP_001298064.1 RING-box protein 2 isoform 2

Status: VALIDATED

Description

Transcript Variant: This variant (2) uses an alternate splice site in the central coding region that results in a frame-shift compared to variant 1. It encodes isoform 2, which is shorter than and has a distinct C-terminus compared to isoform 1.

Source sequence(s)

AC161257, AK003248, AK006703, AK011328, BF018220, BY781065

Consensus CDS

CCDS81056.1

UniProtKB/TrEMBL

D3Z392

Related

ENSMUSP00000108581.2, ENSMUST00000071301.5

Conserved Domains (1) summary

cl17238
Location:28 → 55

RING; RING-finger (Really Interesting New Gene) domain, a specialized type of Zn-finger of 40 to 60 residues that binds two atoms of zinc; defined by the 'cross-brace' motif C-X2-C-X(9-39)-C-X(1-3)- H-X(2-3)-(N/C/H)-X2-C-X(4-48)C-X2-C; probably involved in ...
NM_011279.3 → NP_035409.1 RING-box protein 2 isoform 1

See identical proteins and their annotated locations for NP_035409.1

Status: VALIDATED

Description

Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).

Source sequence(s)

AC161257, AK003248, AK006703, AK011328, BF018220

Consensus CDS

CCDS40730.1

UniProtKB/Swiss-Prot

Q3UKF8, Q9WTZ1

UniProtKB/TrEMBL

D3Z497

Related

ENSMUSP00000052856.5, ENSMUST00000057500.6

Conserved Domains (1) summary

cd16466
Location:47 → 106

RING-H2_RBX2; RING finger, H2 subclass, found in RING-box protein 2 (RBX2) and similar proteins