MTF1 RNA polymerase specificity factor [Saccharomyces cerevisiae S288C] - Gene

Summary

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Gene symbol: MTF1
Gene description: RNA polymerase specificity factor
Primary source: FungiDB:YMR228W
Locus tag: YMR228W
See related: SGD:S000004841; AllianceGenome:SGD:S000004841; VEuPathDB:YMR228W
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary: Enables mitochondrial transcription factor activity. Involved in positive regulation of DNA-templated transcription, elongation and transcription initiation from mitochondrial promoter. Located in mitochondrial intermembrane space and mitochondrial matrix. Part of mitochondrial DNA-directed RNA polymerase complex. [provided by Alliance of Genome Resources, Apr 2022]
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Genomic context

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See MTF1 in Genome Data Viewer

Location:: chromosome: XIII

Exon count:: 1

Sequence:: Chromosome: XIII; NC_001145.3 (724626..725651)

Chromosome XIII - NC_001145.3 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001145.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Cryo-EM Structures Reveal Transcription Initiation Steps by Yeast Mitochondrial RNA Polymerase.
Title: Cryo-EM Structures Reveal Transcription Initiation Steps by Yeast Mitochondrial RNA Polymerase.
The C-terminal tails of the mitochondrial transcription factors Mtf1 and TFB2M are part of an autoinhibitory mechanism that regulates DNA binding.
Title: The C-terminal tails of the mitochondrial transcription factors Mtf1 and TFB2M are part of an autoinhibitory mechanism that regulates DNA binding.
Rpo41-Mtf1 is an attractive candidate for serving as the primase to initiate lagging strand DNA synthesis during normal replication and/or to restart stalled replication from downstream ssDNA.
Title: The Yeast Mitochondrial RNA Polymerase and Transcription Factor Complex Catalyzes Efficient Priming of DNA Synthesis on Single-stranded DNA.
The study examined a series of N-terminal deletion mutants of yeast mitochondrial RNA polymerase, Rpo41, and found that the N-terminal region does not abolish the effects of Mtf1; rather it contributes directly to enzyme catalysis.
Title: Yeast Mitochondrial Transcription Factor Mtf1 Determines the Precision of Promoter-Directed Initiation of RNA Polymerase Rpo41.
This study identifies the importance of the +1+2 AA promoter sequence interactions with Rpo41-Mtf1 in dictating pre-transcribing complex formation, which regulates the binding of initiating NTPs and the catalytic efficiency of transcription initiation.
Title: Interactions of the yeast mitochondrial RNA polymerase with the +1 and +2 promoter bases dictate transcription initiation efficiency.
While Rpo41 induces a slight bend upon binding both the DNAs, addition of the Mtf1 results in severe bending of the promoter and unbending of the non-promoter DNA
Title: Transcription factor-dependent DNA bending governs promoter recognition by the mitochondrial RNA polymerase.
Data show that he promoter DNA is sandwiched between Mtf1 and Rpo41 in the structural model.
Title: Mitochondrial transcription factor Mtf1 traps the unwound non-template strand to facilitate open complex formation.
Data show that both Rpo41p and Mtf1p contact with promoter DNA.
Title: Multiple functions of yeast mitochondrial transcription factor Mtf1p during initiation.
Mtf1 facilitates melting but not recognition of promoters
Title: Intrinsic promoter recognition by a "core" RNA polymerase.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables DNA binding	IEA Inferred from Electronic Annotation more info
enables RNA binding	IEA Inferred from Electronic Annotation more info
enables methyltransferase activity	IEA Inferred from Electronic Annotation more info
enables mitochondrial transcription factor activity	IBA Inferred from Biological aspect of Ancestor more info
enables mitochondrial transcription factor activity	IDA Inferred from Direct Assay more info	PubMed
enables mitochondrial transcription factor activity	IEA Inferred from Electronic Annotation more info
enables mitochondrial transcription factor activity	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
involved_in methylation	IEA Inferred from Electronic Annotation more info
involved_in positive regulation of DNA-templated transcription, elongation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in transcription initiation at mitochondrial promoter	IBA Inferred from Biological aspect of Ancestor more info
involved_in transcription initiation at mitochondrial promoter	IDA Inferred from Direct Assay more info	PubMed
involved_in transcription initiation at mitochondrial promoter	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
part_of mitochondrial DNA-directed RNA polymerase complex	IBA Inferred from Biological aspect of Ancestor more info
part_of mitochondrial DNA-directed RNA polymerase complex	IDA Inferred from Direct Assay more info	PubMed
located_in mitochondrial intermembrane space	IDA Inferred from Direct Assay more info	PubMed
located_in mitochondrial intermembrane space	IEA Inferred from Electronic Annotation more info
is_active_in mitochondrial matrix	IBA Inferred from Biological aspect of Ancestor more info
located_in mitochondrial matrix	IDA Inferred from Direct Assay more info	PubMed
located_in mitochondrion	HDA more info	PubMed
located_in mitochondrion	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: RNA polymerase specificity factor

NP_013955.1

Mitochondrial RNA polymerase specificity factor; has structural similarity to S-adenosylmethionine-dependent methyltransferases and functional similarity to bacterial sigma-factors; Mtf1p interacts with and stabilizes the Rpo41p-promoter complex, enhancing DNA bending and melting to facilitate pre-initiation open complex formation