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    Tent2 terminal nucleotidyltransferase 2 [ Mus musculus (house mouse) ]

    Gene ID: 100715, updated on 2-Nov-2024

    Summary

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    Official Symbol
    Tent2provided by MGI
    Official Full Name
    terminal nucleotidyltransferase 2provided by MGI
    Primary source
    MGI:MGI:2140950
    See related
    Ensembl:ENSMUSG00000042167 AllianceGenome:MGI:2140950
    Gene type
    protein coding
    RefSeq status
    VALIDATED
    Organism
    Mus musculus
    Lineage
    Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
    Also known as
    Gld2; GLD-2; Papd4; 8030446C20Rik
    Summary
    Enables poly(A) RNA polymerase activity. Involved in target-directed miRNA degradation. Acts upstream of or within hematopoietic progenitor cell differentiation. Located in nucleus. Is expressed in embryo. Orthologous to human TENT2 (terminal nucleotidyltransferase 2). [provided by Alliance of Genome Resources, Nov 2024]
    Expression
    Ubiquitous expression in limb E14.5 (RPKM 18.2), placenta adult (RPKM 16.4) and 28 other tissues See more
    Orthologs
    human all
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    Genomic context

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    See Tent2 in Genome Data Viewer
    Location:
    13 C3; 13 47.81 cM
    Exon count:
    17
    Annotation release Status Assembly Chr Location
    RS_2024_02 current GRCm39 (GCF_000001635.27) 13 NC_000079.7 (93282363..93330662, complement)
    108.20200622 previous assembly GRCm38.p6 (GCF_000001635.26) 13 NC_000079.6 (93145855..93192435, complement)

    Chromosome 13 - NC_000079.7Genomic Context describing neighboring genes Neighboring gene cardiomyopathy associated 5 Neighboring gene RIKEN cDNA A630019I02 gene Neighboring gene CapStarr-seq enhancer MGSCv37_chr13:93864100-93864343 Neighboring gene predicted gene 4814 Neighboring gene CapStarr-seq enhancer MGSCv37_chr13:93864596-93864798 Neighboring gene STARR-seq mESC enhancer starr_35275 Neighboring gene STARR-seq mESC enhancer starr_35276 Neighboring gene STARR-seq mESC enhancer starr_35278 Neighboring gene predicted gene, 41020 Neighboring gene STARR-seq mESC enhancer starr_35279 Neighboring gene STARR-seq mESC enhancer starr_35280 Neighboring gene STARR-positive B cell enhancer mm9_chr13:94013261-94013562 Neighboring gene glyceraldehyde-3-phosphate dehydrogenase pseudogene

    Genomic regions, transcripts, and products

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    Go to nucleotide: Graphics FASTA GenBank

    Expression

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    • Project title: Mouse ENCODE transcriptome data Mouse ENCODE transcriptome data
    • Description: RNA profiling data sets generated by the Mouse ENCODE project.
    • BioProject: PRJNA66167
    • Publication: PMID 25409824
    • Analysis date: n/a

    Bibliography

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    Related articles in PubMed

    1. Gld2-catalyzed 3' monoadenylation of miRNAs in the hippocampus has no detectable effect on their stability or on animal behavior. Mansur F, et al. RNA, 2016 Oct. PMID 27495319, Free PMC Article
    2. Possible role of mouse poly(A) polymerase mGLD-2 during oocyte maturation. Nakanishi T, et al. Dev Biol, 2006 Jan 1. PMID 16325797
    3. Selective stabilization of mammalian microRNAs by 3' adenylation mediated by the cytoplasmic poly(A) polymerase GLD-2. Katoh T, et al. Genes Dev, 2009 Feb 15. PMID 19240131, Free PMC Article
    4. Noncanonical cytoplasmic poly(A) polymerases regulate RNA levels, alternative RNA processing, and synaptic plasticity but not hippocampal-dependent behaviours. Mansur F, et al. RNA Biol, 2021 Jul. PMID 32954964, Free PMC Article
    5. Musashi protein-directed translational activation of target mRNAs is mediated by the poly(A) polymerase, germ line development defective-2. Cragle C, et al. J Biol Chem, 2014 May 16. PMID 24644291, Free PMC Article

    See all (27) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Noncanonical cytoplasmic poly(A) polymerases regulate RNA levels, alternative RNA processing, and synaptic plasticity but not hippocampal-dependent behaviours.
      Title: Noncanonical cytoplasmic poly(A) polymerases regulate RNA levels, alternative RNA processing, and synaptic plasticity but not hippocampal-dependent behaviours.
    2. In Gld2 knockout mice there are detectable alterations in specific miRNA monoadenylation in the hippocampus, but no detectable effect on miRNA stability and no overt change in animal behavior.
      Title: Gld2-catalyzed 3' monoadenylation of miRNAs in the hippocampus has no detectable effect on their stability or on animal behavior.
    3. poly(A) polymerase Gld2, deadenylase PARN, and translation inhibitory factor neuroguidin (Ngd) are components of a dendritic CPEB-associated polyadenylation apparatus
      Title: Bidirectional control of mRNA translation and synaptic plasticity by the cytoplasmic polyadenylation complex.
    4. We found an association of mGLD-2 with cytoplasmic polyadenylation components, CPEB and CPSF described in Xenopus oocytes
      Title: Disruption of mouse poly(A) polymerase mGLD-2 does not alter polyadenylation status in oocytes and somatic cells.
    5. mGLD-2 may act in the ooplasm on the progression of metaphase I to metaphase II during oocyte maturation.
      Title: Possible role of mouse poly(A) polymerase mGLD-2 during oocyte maturation.
    6. identified GLD-2, which is a regulatory cytoplasmic poly(A) polymerase, as responsible for the 3'-terminal adenylation of miR-122 after unwinding of the miR-122/miR-122* duplex
      Title: Selective stabilization of mammalian microRNAs by 3' adenylation mediated by the cytoplasmic poly(A) polymerase GLD-2.
    Submit: New GeneRIF Correction

    Variation

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    Alleles

    Alleles of this type are documented at Mouse Genome Informatics  (MGI)
    • Endonuclease-mediated (3) 
    • Targeted (1)  1 citation

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Markers

    Homology

    Gene Ontology Provided by MGI

    Function Evidence Code Pubs
    enables ATP binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables metal ion binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables poly(A) RNA polymerase activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables poly(A) RNA polymerase activity IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    enables poly(A) RNA polymerase activity ISO
    Inferred from Sequence Orthology
    more info
    		  
    enables poly(A) RNA polymerase activity ISS
    Inferred from Sequence or Structural Similarity
    more info
    		  
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    Process Evidence Code Pubs
    involved_in RNA 3'-end processing IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in RNA polyadenylation at postsynapse ISO
    Inferred from Sequence Orthology
    more info
    		  
    acts_upstream_of_or_within hematopoietic progenitor cell differentiation IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in histone mRNA catabolic process ISO
    Inferred from Sequence Orthology
    more info
    		  
    involved_in histone mRNA catabolic process ISS
    Inferred from Sequence or Structural Similarity
    more info
    		  
    involved_in mRNA 3'-end processing IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in mRNA 3'-end processing ISO
    Inferred from Sequence Orthology
    more info
    		  
    involved_in negative regulation of miRNA catabolic process ISO
    Inferred from Sequence Orthology
    more info
    		  
    involved_in negative regulation of miRNA catabolic process ISS
    Inferred from Sequence or Structural Similarity
    more info
    		  
    involved_in positive regulation of protein targeting to membrane ISO
    Inferred from Sequence Orthology
    more info
    		  
    involved_in postsynaptic modulation of chemical synaptic transmission ISO
    Inferred from Sequence Orthology
    more info
    		  
    involved_in regulation of translation at postsynapse ISO
    Inferred from Sequence Orthology
    more info
    		  
    involved_in target-directed miRNA degradation IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    Component Evidence Code Pubs
    located_in cytoplasm IEA
    Inferred from Electronic Annotation
    more info
    		  
    is_active_in glutamatergic synapse ISO
    Inferred from Sequence Orthology
    more info
    		  
    located_in nucleus IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    is_active_in perforant pathway to dendrate granule cell synapse ISO
    Inferred from Sequence Orthology
    more info
    		  
    is_active_in postsynapse ISO
    Inferred from Sequence Orthology
    more info
    		  

    General protein information

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    Preferred Names
    poly(A) RNA polymerase GLD2
    Names
    PAP associated domain containing 4
    PAP-associated domain-containing protein 4
    cytoplasmic poly(A) polymerase
    NP_001348465.1
    NP_001348466.1
    NP_598666.1
    XP_006517573.1
    XP_030102937.1

    NCBI Reference Sequences (RefSeq)

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    RefSeqs maintained independently of Annotated Genomes

    These reference sequences exist independently of genome builds. Explain

    These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

    mRNA and Protein(s)

    1. NM_001361536.1 → NP_001348465.1  poly(A) RNA polymerase GLD2 isoform 2

      Status: VALIDATED

      Source sequence(s)
      AC120347, AC121991, AC130217
      Consensus CDS
      CCDS88498.1
      Related
      ENSMUSP00000153703.2, ENSMUST00000225868.2
      Conserved Domains (1) summary
      COG5260
      Location:121 → 464
      TRF4; DNA polymerase sigma [Replication, recombination and repair]

    2. NM_001361537.1 → NP_001348466.1  poly(A) RNA polymerase GLD2 isoform 1

      Status: VALIDATED

      Source sequence(s)
      AC120347, AC121991, AC130217
      Consensus CDS
      CCDS26686.1
      UniProtKB/Swiss-Prot
      A4F5H0, Q8CCH5, Q91YI6
      Conserved Domains (1) summary
      COG5260
      Location:121 → 468
      TRF4; DNA polymerase sigma [Replication, recombination and repair]

    3. NM_133905.3 → NP_598666.1  poly(A) RNA polymerase GLD2 isoform 1

      See identical proteins and their annotated locations for NP_598666.1

      Status: VALIDATED

      Source sequence(s)
      AC120347, AC121991, AC130217
      Consensus CDS
      CCDS26686.1
      UniProtKB/Swiss-Prot
      A4F5H0, Q8CCH5, Q91YI6
      Related
      ENSMUSP00000048124.6, ENSMUST00000048702.7
      Conserved Domains (1) summary
      COG5260
      Location:121 → 468
      TRF4; DNA polymerase sigma [Replication, recombination and repair]

    RNA

    1. NR_154997.1 RNA Sequence

      Status: VALIDATED

      Source sequence(s)
      AC120347, AC121991, AC130217

    RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference GRCm39 C57BL/6J

    Genomic

    1. NC_000079.7 Reference GRCm39 C57BL/6J

      Range
      93282363..93330662 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. XM_030247077.2 → XP_030102937.1  poly(A) RNA polymerase GLD2 isoform X2

      Conserved Domains (1) summary
      COG5260
      Location:121 → 464
      TRF4; DNA polymerase sigma [Replication, recombination and repair]

    2. XM_006517510.5 → XP_006517573.1  poly(A) RNA polymerase GLD2 isoform X1

      See identical proteins and their annotated locations for XP_006517573.1

      UniProtKB/Swiss-Prot
      A4F5H0, Q8CCH5, Q91YI6
      Conserved Domains (1) summary
      COG5260
      Location:121 → 468
      TRF4; DNA polymerase sigma [Replication, recombination and repair]

    RNA

    1. XR_004937923.1 RNA Sequence

    2. XR_001780738.3 RNA Sequence

    Nucleotide Protein Strain
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    Q91YI6.1 GenPept UniProtKB/Swiss-Prot:Q91YI6

    Gene LinkOut

    The following LinkOut resources are supplied by external providers. These providers are responsible for maintaining the links.

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