CDC50 aminophospholipid translocase regulatory protein CDC50 [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: CDC50
Official Full Name: aminophospholipid translocase regulatory protein CDC50
Primary source: SGD:S000000690
Locus tag: YCR094W
See related: AllianceGenome:SGD:S000000690; FungiDB:YCR094W; VEuPathDB:YCR094W
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary: Contributes to aminophospholipid flippase activity. Involved in several processes, including actin cortical patch localization; phospholipid translocation; and retrograde transport, endosome to Golgi. Located in late endosome membrane and trans-Golgi network. Part of Cdc50p-Drs2p complex. Orthologous to several human genes including TMEM30B (transmembrane protein 30B). [provided by Alliance of Genome Resources, Nov 2024]
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Genomic context

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See CDC50 in Genome Data Viewer

Location:: chromosome: III

Exon count:: 1

Sequence:: Chromosome: III; NC_001135.5 (286762..287937)

Chromosome III - NC_001135.5 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001135.5

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

cryo-electron microscopy structure of the P4-ATPase Drs2p-Cdc50p; three structures are presented that represent the complex in an autoinhibited, an intermediate and a fully activated state
Title: Structure and autoregulation of a P4-ATPase lipid flippase.
Cdc50p, a membrane protein in the endosomal/trans-Golgi network compartments, is a noncatalytic subunit of Drs2p, which is implicated in translocation of phospholipids across lipid bilayers. (cdc50p)
Title: Defects in structural integrity of ergosterol and the Cdc50p-Drs2p putative phospholipid translocase cause accumulation of endocytic membranes, onto which actin patches are assembled in yeast.
activation of the Drs2p-Cdc50p flippase follows a multistep mechanism, with preliminary release of a number of constraints, possibly through the binding of regulatory proteins in the trans-Golgi network, followed by full activation by PI4P
Title: High phosphatidylinositol 4-phosphate (PI4P)-dependent ATPase activity for the Drs2p-Cdc50p flippase after removal of its N- and C-terminal extensions.
we describe the overexpression of a yeast lipid flippase complex, namely the P4-ATPase Drs2p and its associated subunit Cdc50p, in a coordinated manner.
Title: Coordinated Overexpression in Yeast of a P4-ATPase and Its Associated Cdc50 Subunit: The Case of the Drs2p/Cdc50p Lipid Flippase Complex.
Phosphatidylinositol-4-phosphate tightly controls Drs2p-catalyzed lipid transport and establish the functional relevance of the Drs2p.Cdc50p complex.
Title: Phosphatidylserine stimulation of Drs2p·Cdc50p lipid translocase dephosphorylation is controlled by phosphatidylinositol-4-phosphate.
ATP9B is able to exit the ER and localize to the TGN independently of CDC50 proteins and this protein contains a Golgi localization signal in its N-terminal cytoplasmic region
Title: ATP9B, a P4-ATPase (a putative aminophospholipid translocase), localizes to the trans-Golgi network in a CDC50 protein-independent manner.
Cdc50 proteins are integral components of the P(4)-ATPase transport machinery
Title: Cdc50p plays a vital role in the ATPase reaction cycle of the putative aminophospholipid transporter Drs2p.
These results suggest that Cdc50p-Drs2p plays an important role in the Arf1p/Gcs1-mediated formation of clathrin-coated vesicles for the retrieval pathway from early endosomes to the trans-Golgi network.
Title: The functional relationship between the Cdc50p-Drs2p putative aminophospholipid translocase and the Arf GAP Gcs1p in vesicle formation in the retrieval pathway from yeast early endosomes to the TGN.
Drs2 or cdc50 null mutatations cause a loss of plasma membrane PS asymmetry. Loss of plasma membrane asymmetry is a secondary consequence of disrupting protein trafficking.
Title: Roles for the Drs2p-Cdc50p complex in protein transport and phosphatidylserine asymmetry of the yeast plasma membrane.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
contributes_to aminophospholipid flippase activity	IGI Inferred from Genetic Interaction more info	PubMed

Process	Evidence Code	Pubs
involved_in actin cortical patch localization	IGI Inferred from Genetic Interaction more info	PubMed
involved_in aminophospholipid translocation	IEA Inferred from Electronic Annotation more info
involved_in endocytosis	IGI Inferred from Genetic Interaction more info	PubMed
involved_in intracellular protein transport	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in phospholipid translocation	IGI Inferred from Genetic Interaction more info	PubMed
involved_in retrograde transport, endosome to Golgi	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
part_of Cdc50p-Drs2p complex	IPI Inferred from Physical Interaction more info	PubMed
is_active_in Golgi apparatus	IBA Inferred from Biological aspect of Ancestor more info
located_in Golgi apparatus	IEA Inferred from Electronic Annotation more info
located_in cytosol	IEA Inferred from Electronic Annotation more info
located_in endoplasmic reticulum	HDA more info	PubMed
is_active_in endoplasmic reticulum	IBA Inferred from Biological aspect of Ancestor more info
located_in endosome	IEA Inferred from Electronic Annotation more info
located_in late endosome membrane	IDA Inferred from Direct Assay more info	PubMed
located_in late endosome membrane	IEA Inferred from Electronic Annotation more info
located_in membrane	IEA Inferred from Electronic Annotation more info
is_active_in plasma membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in trans-Golgi network	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: aminophospholipid translocase regulatory protein CDC50

NP_010018.1

Endosomal protein that interacts with phospholipid flippase Drs2p; interaction with Cdc50p is essential for Drs2p catalytic activity; mutations affect cell polarity and polarized growth; similar to Lem3p; CDC50 has a paralog, YNR048W, that arose from the whole genome duplication

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001135.5 Reference assembly

Range

286762..287937

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001178800.1 → NP_010018.1 TPA: aminophospholipid translocase regulatory protein CDC50 [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_010018.1

Status: REVIEWED

UniProtKB/Swiss-Prot

D6VR94, P25656

UniProtKB/TrEMBL

B3LUE6, C8Z4I0

Conserved Domains (1) summary

COG5035
Location:1 → 369

CDC50; Cell cycle control protein [Cell division and chromosome partitioning / Transcription / Signal transduction mechanisms]