MOT1 [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: MOT1
Primary source: SGD:S000006003
Locus tag: YPL082C
See related: AllianceGenome:SGD:S000006003; FungiDB:YPL082C; VEuPathDB:YPL082C
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as: BUR3; END10; LPF4
Summary: Enables ATP hydrolysis activity; DNA binding activity; and TBP-class protein binding activity. Involved in RNA metabolic process and regulation of transcription, DNA-templated. Located in nuclear chromosome. Orthologous to human BTAF1 (B-TFIID TATA-box binding protein associated factor 1). [provided by Alliance of Genome Resources, Apr 2022]
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Genomic context

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Location:: chromosome: XVI

Exon count:: 1

Sequence:: Chromosome: XVI; NC_001148.4 (398480..404083, complement)

Chromosome XVI - NC_001148.4 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001148.4

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Connection of core and tail Mediator modules restrains transcription from TFIID-dependent promoters.
Title: Connection of core and tail Mediator modules restrains transcription from TFIID-dependent promoters.
A framework for understanding how the efficiency of Mot1's catalytic activity is tuned to establish a dynamic pool of TBP without interfering with stable and functional TBP-containing complexes.
Title: Conformational changes and catalytic inefficiency associated with Mot1-mediated TBP-DNA dissociation.
These results indicate that Rpa49, Rrn3 and Mot1 directly or indirectly influence biogenesis
Title: Identification of factors that promote biogenesis of tRNA(CGA)(Ser).
Mot1, Ino80 chromatin remodeling complex (Ino80C), and NC2 co-localize on chromatin and coordinately suppress pervasive transcription in S. cerevisiae.
Title: Mot1, Ino80C, and NC2 Function Coordinately to Regulate Pervasive Transcription in Yeast and Mammals.
these results suggest that TBP-DNA affinity as well as other aspects of promoter sequence influence Mot1 function in vivo.
Title: Molecular Mechanism of Mot1, a TATA-binding Protein (TBP)-DNA Dissociating Enzyme.
These results suggest that the large scale overlap in Mot1 and Spt16 function arises from a combination of both their unique and shared functions in transcription complex assembly and chromatin structure regulation.
Title: The Modifier of Transcription 1 (Mot1) ATPase and Spt16 Histone Chaperone Co-regulate Transcription through Preinitiation Complex Assembly and Nucleosome Organization.
The results stress the importance of the cooperation of Mot1p/NC2 with remodelers in maintaining a repressive environment for intragenic transcription.
Title: Suppression of intragenic transcription requires the MOT1 and NC2 regulators of TATA-binding protein.
The action of Mot1 is required to clear TBP from intrinsically preferred (TATA-containing) binding sites, ensuring sufficient soluble TBP to bind intrinsically disfavored (TATA-less) sites.
Title: Mot1 redistributes TBP from TATA-containing to TATA-less promoters.
In the absence of ATP, ternary complex stability arises from both Mot1-DNA and Mot1-TBP interactions.
Title: Two-step mechanism for modifier of transcription 1 (Mot1) enzyme-catalyzed displacement of TATA-binding protein (TBP) from DNA.
findings suggest that the four conserved regions of Mot1 are essential for viability and required for proper regulation of most Mot1-regulated genes; loss of the ATPase domain, however, imparts some unexpected regulation on certain genes
Title: Genome-wide transcriptional dependence on conserved regions of Mot1.

Submit: New GeneRIF Correction See all GeneRIFs (22)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables ATP hydrolysis activity	IDA Inferred from Direct Assay more info	PubMed
enables ATP hydrolysis activity	IEA Inferred from Electronic Annotation more info
enables ATP-dependent chromatin remodeler activity	IEA Inferred from Electronic Annotation more info
enables DNA binding	IDA Inferred from Direct Assay more info	PubMed
enables DNA binding	IEA Inferred from Electronic Annotation more info
enables TBP-class protein binding	IEA Inferred from Electronic Annotation more info
enables TBP-class protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables helicase activity	IEA Inferred from Electronic Annotation more info
enables hydrolase activity	IEA Inferred from Electronic Annotation more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
involved_in chromatin remodeling	IEA Inferred from Electronic Annotation more info
involved_in negative regulation of DNA-templated transcription	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in nucleolar large rRNA transcription by RNA polymerase I	IDA Inferred from Direct Assay more info	PubMed
involved_in nucleolar large rRNA transcription by RNA polymerase I	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in rRNA processing	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of RNA polymerase II transcription preinitiation complex assembly	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of transcription by RNA polymerase II	IDA Inferred from Direct Assay more info	PubMed

Component	Evidence Code	Pubs
located_in mitochondrion	HDA more info	PubMed
located_in mitochondrion	IEA Inferred from Electronic Annotation more info
located_in nuclear chromosome	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	HDA more info	PubMed
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: DNA-binding ATPase

NP_015243.1

Essential protein involved in regulation of transcription; removes Spt15p (TBP) from DNA via its C-terminal ATPase activity; may have a role in ensuring that soluble TBP is available to bind TATA-less promoters; forms a complex with TBP that binds TATA DNA with high affinity but with altered specificity; the Mot1p-Spt15p-DNA ternary complex contains unbent DNA; coregulates transcription with Spt16p through assembly of preinitiation complex and organization of nucleosomes

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001148.4 Reference assembly

Range

398480..404083 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001183896.1 → NP_015243.1 TPA: DNA-binding ATPase [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_015243.1

Status: REVIEWED

UniProtKB/Swiss-Prot

D6W3T5, P32333

UniProtKB/TrEMBL

A6ZWG9, B3LKZ0

Conserved Domains (4) summary

COG0553
Location:1011 → 1803

HepA; Superfamily II DNA or RNA helicase, SNF2 family [Transcription, Replication, recombination and repair]

sd00044
Location:360 → 385

HEAT; HEAT repeat [structural motif]

cd17999
Location:1272 → 1507

DEXHc_Mot1; DEXH-box helicase domain of Mot1

pfam12054
Location:611 → 1063

DUF3535; Domain of unknown function (DUF3535)