HBS1 ribosome dissociation factor GTPase HBS1 [Saccharomyces cerevisiae S288C] - Gene

Summary

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Gene symbol: HBS1
Gene description: ribosome dissociation factor GTPase HBS1
Primary source: FungiDB:YKR084C
Locus tag: YKR084C
See related: SGD:S000001792; AllianceGenome:SGD:S000001792; VEuPathDB:YKR084C
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary: Enables GTPase activity. Contributes to ribosome binding activity. Involved in RNA catabolic process; positive regulation of translation; and ribosome disassembly. Located in cytosol. Part of Dom34-Hbs1 complex. Human ortholog(s) of this gene implicated in beta thalassemia. Orthologous to human HBS1L (HBS1 like translational GTPase). [provided by Alliance of Genome Resources, Apr 2022]
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Genomic context

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See HBS1 in Genome Data Viewer

Location:: chromosome: XI

Exon count:: 1

Sequence:: Chromosome: XI; NC_001143.9 (597055..598890, complement)

Chromosome XI - NC_001143.9 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001143.9

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

analysis establishes the structural foundation for the recognition of aberrantly stalled 80S ribosomes by the Dom34.Hbs1.GTP complex during Dom34-mediated mRNA surveillance pathways
Title: Structural insights into ribosomal rescue by Dom34 and Hbs1 at near-atomic resolution.
Roles of dom34:hbs1 in nonstop protein clearance from translocators for normal organelle protein influx.
Title: Roles of dom34:hbs1 in nonstop protein clearance from translocators for normal organelle protein influx.
Dom34:Hbs1 facilitates the decay of nonstop mRNAs from the 3' end by exosomes and is required for the complete degradation of nonstop mRNA decay intermediates.
Title: Dom34:hbs1 plays a general role in quality-control systems by dissociation of a stalled ribosome at the 3' end of aberrant mRNA.
The close structural similarity of Dom34 and Hbs1 to eukaryotic release factors (eRFs) enabled the authors to propose a model for the ribosome-bound eRF1-eRF3 complex.
Title: Structure of the no-go mRNA decay complex Dom34-Hbs1 bound to a stalled 80S ribosome.
findings show that Dom34:Hbs1 (two interacting factors genetically implicated in no-go decay) interacts with the ribosome to promote subunit dissociation and peptidyl-tRNA drop-off
Title: Dom34:Hbs1 promotes subunit dissociation and peptidyl-tRNA drop-off to initiate no-go decay.
Dom34 has a role in an mRNA decay pathway called No-Go decay and the interaction of Dom34 with Hbs1 results in an increase in the affinity constant of Hbs1 for GTP but not GDP
Title: Structure of yeast Dom34: a protein related to translation termination factor Erf1 and involved in No-Go decay.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables GTP binding	IEA Inferred from Electronic Annotation more info
enables GTPase activity	IBA Inferred from Biological aspect of Ancestor more info
enables GTPase activity	IDA Inferred from Direct Assay more info	PubMed
enables GTPase activity	IEA Inferred from Electronic Annotation more info
enables hydrolase activity	IEA Inferred from Electronic Annotation more info
contributes_to ribosome binding	IDA Inferred from Direct Assay more info	PubMed
enables translation elongation factor activity	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in nonfunctional rRNA decay	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in nuclear-transcribed mRNA catabolic process, no-go decay	IGI Inferred from Genetic Interaction more info	PubMed
involved_in positive regulation of translation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of translation	IEA Inferred from Electronic Annotation more info
involved_in ribosome disassembly	IDA Inferred from Direct Assay more info	PubMed
involved_in translation	IBA Inferred from Biological aspect of Ancestor more info
involved_in translation	IEA Inferred from Electronic Annotation more info
involved_in translational elongation	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
part_of Dom34-Hbs1 complex	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasm	HDA more info	PubMed
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
located_in cytosol	HDA more info	PubMed

General protein information

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Preferred Names: ribosome dissociation factor GTPase HBS1

NP_013010.3

GTPase with similarity to translation release factors; together with binding partner Dom34p, facilitates ribosomal subunit dissociation and peptidyl-tRNA release when translation is stalled, particularly in 3' UTRs; genetically implicated in mRNA no-go decay; HBS1 has a paralog, SKI7, that arose from the whole genome duplication

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001143.9 Reference assembly

Range

597055..598890 complement

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001179874.3 → NP_013010.3 TPA: ribosome dissociation factor GTPase HBS1 [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_013010.3

Status: REVIEWED

UniProtKB/Swiss-Prot

D6VXE4, P32769

UniProtKB/TrEMBL

A0A8H4FAE3, A7A043, B3LRF8, B5VMM6, C7GXC6, C8ZCP5

Conserved Domains (2) summary

COG5256
Location:161 → 611

TEF1; Translation elongation factor EF-1alpha (GTPase) [Translation, ribosomal structure and biogenesis]

pfam08938
Location:11 → 73

HBS1_N; HBS1 N-terminus