HSH155 [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: HSH155
Primary source: SGD:S000004901
Locus tag: YMR288W
See related: AllianceGenome:SGD:S000004901; FungiDB:YMR288W; VEuPathDB:YMR288W
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary: Enables mRNA binding activity. Involved in spliceosomal complex assembly. Part of U2 snRNP and U2-type prespliceosome. Used to study myelodysplastic syndrome. Human ortholog(s) of this gene implicated in hepatocellular carcinoma and myelodysplastic syndrome. Orthologous to human SF3B1 (splicing factor 3b subunit 1). [provided by Alliance of Genome Resources, Apr 2022]
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Genomic context

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Location:: chromosome: XIII

Exon count:: 1

Sequence:: Chromosome: XIII; NC_001145.3 (845571..848486)

Chromosome XIII - NC_001145.3 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001145.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Cdc48 regulates intranuclear quality control sequestration of the Hsh155 splicing factor in budding yeast.
Title: Cdc48 regulates intranuclear quality control sequestration of the Hsh155 splicing factor in budding yeast.
Results show the identification of a potential UHM-ULM protein domains interaction between Cus2 and Hsh155. Amino acid substitutions in the ULM of Hsh155 abolish the binding in vivo.
Title: Cus2 enforces the first ATP-dependent step of splicing by binding to yeast SF3b1 through a UHM-ULM interaction.
Selective aggregation of the splicing factor Hsh155 suppresses splicing upon genotoxic stress.
Title: Selective aggregation of the splicing factor Hsh155 suppresses splicing upon genotoxic stress.
different HSH155 alleles elicit disparate effects on splicing: some increase the fidelity of BS selection while others decrease fidelity. Our data support a model wherein conformational changes in SF3b1 promote U2 association with the BS independently of the action of the DEAD-box ATPase Prp5. We propose that SF3b1 functions to stabilize weak U2/BS duplexes to drive spliceosome assembly and splicing
Title: SF3b1 mutations associated with myelodysplastic syndromes alter the fidelity of branchsite selection in yeast.
show here that hsh155 mutant alleles in Saccharomyces cerevisiae, counterparts of SF3B1 mutations frequently found in cancers, specifically change splicing of suboptimal BS pre-mRNA substrates
Title: SF3B1/Hsh155 HEAT motif mutations affect interaction with the spliceosomal ATPase Prp5, resulting in altered branch site selectivity in pre-mRNA splicing.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables mRNA binding	IBA Inferred from Biological aspect of Ancestor more info
enables mRNA binding	IDA Inferred from Direct Assay more info	PubMed
enables mRNA binding	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in RNA splicing	IEA Inferred from Electronic Annotation more info
involved_in mRNA processing	IEA Inferred from Electronic Annotation more info
involved_in mRNA splicing, via spliceosome	IPI Inferred from Physical Interaction more info	PubMed
involved_in spliceosomal complex assembly	IBA Inferred from Biological aspect of Ancestor more info
involved_in spliceosomal complex assembly	IEA Inferred from Electronic Annotation more info
involved_in spliceosomal complex assembly	IPI Inferred from Physical Interaction more info	PubMed

Component	Evidence Code	Pubs
part_of U2 snRNP	IBA Inferred from Biological aspect of Ancestor more info
part_of U2 snRNP	IDA Inferred from Direct Assay more info	PubMed
part_of U2-type prespliceosome	IBA Inferred from Biological aspect of Ancestor more info
part_of U2-type prespliceosome	IDA Inferred from Direct Assay more info	PubMed
part_of catalytic step 2 spliceosome	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleus	IEA Inferred from Electronic Annotation more info
part_of spliceosomal complex	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: U2 snRNP complex subunit HSH155

NP_014015.1

U2-snRNP associated splicing factor; forms extensive associations with the branch site-3' splice site-3' exon region upon prespliceosome formation; similarity to the mammalian U2 snRNP-associated splicing factor SAP155

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001145.3 Reference assembly

Range

845571..848486

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001182795.1 → NP_014015.1 TPA: U2 snRNP complex subunit HSH155 [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_014015.1

Status: REVIEWED

UniProtKB/Swiss-Prot

D6W0B5, P49955

UniProtKB/TrEMBL

G2WKZ7, N1NZ66

Conserved Domains (2) summary

COG5181
Location:1 → 971

HSH155; U2 snRNP spliceosome subunit [RNA processing and modification]

sd00044
Location:519 → 543

HEAT; HEAT repeat [structural motif]