BUD23 18S rRNA (guanine1575-N7)-methyltransferase [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: BUD23
Official Full Name: 18S rRNA (guanine1575-N7)-methyltransferase
Primary source: SGD:S000000643
Locus tag: YCR047C
See related: AllianceGenome:SGD:S000000643; FungiDB:YCR047C; VEuPathDB:YCR047C
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary: Enables rRNA (guanine) methyltransferase activity. Involved in endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA); rRNA (guanine-N7)-methylation; and ribosomal small subunit export from nucleus. Located in nucleolus. Human ortholog(s) of this gene implicated in Williams-Beuren syndrome. Orthologous to human BUD23 (BUD23 rRNA methyltransferase and ribosome maturation factor). [provided by Alliance of Genome Resources, Apr 2022]
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Genomic context

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See BUD23 in Genome Data Viewer

Location:: chromosome: III

Exon count:: 1

Sequence:: Chromosome: III; NC_001135.5 (210718..211545, complement)

Chromosome III - NC_001135.5 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001135.5

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Release of the ribosome biogenesis factor Bud23 from small subunit precursors in yeast.
Title: Release of the ribosome biogenesis factor Bud23 from small subunit precursors in yeast.
Bud23 promotes the final disassembly of the small subunit Processome in Saccharomyces cerevisiae.
Title: Bud23 promotes the final disassembly of the small subunit Processome in Saccharomyces cerevisiae.
BUD23 works at the transition of early to middle stage of 40S biogenesis.
Title: The rRNA methyltransferase Bud23 shows functional interaction with components of the SSU processome and RNase MRP.
The absence of Bud23, Bud23 (G57R) at a high level might affect the cell division of yeast cells through an as yet unidentified mechanism.
Title: New phenotypes generated by the G57R mutation of BUD23 in Saccharomyces cerevisiae.
Mutations in Bud23 that inactivated its methyltransferase activity complemented a bud23Delta mutant. In addition, mutant ribosomes in which G1575 was changed to adenosine supported growth comparable to that of cells with wild-type ribosomes.
Title: Bud23 methylates G1575 of 18S rRNA and is required for efficient nuclear export of pre-40S subunits.

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Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables methyltransferase activity	IEA Inferred from Electronic Annotation more info
enables rRNA (guanine) methyltransferase activity	IBA Inferred from Biological aspect of Ancestor more info
enables rRNA (guanine) methyltransferase activity	IEA Inferred from Electronic Annotation more info
enables rRNA (guanine) methyltransferase activity	IMP Inferred from Mutant Phenotype more info	PubMed

Process	Evidence Code	Pubs
involved_in endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in methylation	IEA Inferred from Electronic Annotation more info
involved_in rRNA (guanine-N7)-methylation	IBA Inferred from Biological aspect of Ancestor more info
involved_in rRNA (guanine-N7)-methylation	IEA Inferred from Electronic Annotation more info
involved_in rRNA (guanine-N7)-methylation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in rRNA processing	IEA Inferred from Electronic Annotation more info
involved_in ribosomal small subunit export from nucleus	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in cytoplasm	HDA more info	PubMed
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
is_active_in nucleolus	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleolus	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	HDA more info	PubMed
located_in nucleus	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: 18S rRNA (guanine1575-N7)-methyltransferase

NP_009976.1

EC 2.1.1.309

Ribosome biogenesis factor; methyltransferase that methylates residue G1575 of 18S rRNA; required for rRNA processing and nuclear export of 40S ribosomal subunits independently of methylation activity; required for recruitment of Rps2p; functions with DEAH-box RNA helicase Ecm16p; diploid mutant displays random budding pattern; functional homolog of human WBSCR22

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001135.5 Reference assembly

Range

210718..211545 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001178761.1 → NP_009976.1 TPA: 18S rRNA (guanine1575-N7)-methyltransferase [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_009976.1

Status: REVIEWED

UniProtKB/Swiss-Prot

D6VR56, P25627

UniProtKB/TrEMBL

A6ZTM8, B3LUB2, C7GJT8, C8Z4D8, G2WA64, N1PAM9

Conserved Domains (2) summary

pfam12589
Location:226 → 273

WBS_methylT; Methyltransferase involved in Williams-Beuren syndrome

pfam13649
Location:51 → 152

Methyltransf_25; Methyltransferase domain