PDI1 protein disulfide isomerase PDI1 [Saccharomyces cerevisiae S288C] - Gene

Summary

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Gene symbol: PDI1
Gene description: protein disulfide isomerase PDI1
Primary source: FungiDB:YCL043C
Locus tag: YCL043C
See related: SGD:S000000548; AllianceGenome:SGD:S000000548; VEuPathDB:YCL043C
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as: MFP1; TRG1
Summary: Enables protein disulfide isomerase activity; protein-disulfide reductase activity; and unfolded protein binding activity. Involved in mannose trimming involved in glycoprotein ERAD pathway and protein folding. Located in endoplasmic reticulum lumen. Human ortholog(s) of this gene implicated in Cole-Carpenter syndrome and autoimmune hepatitis. Orthologous to several human genes including PDIA2 (protein disulfide isomerase family A member 2); PDIA3 (protein disulfide isomerase family A member 3); and PDIA4 (protein disulfide isomerase family A member 4). [provided by Alliance of Genome Resources, Apr 2022]
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Genomic context

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See PDI1 in Genome Data Viewer

Location:: chromosome: III

Exon count:: 1

Sequence:: Chromosome: III; NC_001135.5 (48653..50221, complement)

Chromosome III - NC_001135.5 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001135.5

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

The results suggest that PDI flexibility enables it to interact with many different partner molecules of widely different sizes and shapes.
Title: The flexibility and dynamics of protein disulfide isomerase.
Data show that binding of mannosidase Htm1 to protein disulfide isomerase Pdi1 is required for endoplasmic reticulum (ER)-associated protein degradation (ERAD).
Title: A Complex of Htm1 and the Oxidoreductase Pdi1 Accelerates Degradation of Misfolded Glycoproteins.
ERO1 is enzymatically active even with its regulatory disulfides intact, and further activation of ERO1 by reduction of the regulatory disulfides requires the reduction of non-catalytic Cys(90)-Cys(97)disulfide in PDI1.
Title: Novel Roles of the Non-catalytic Elements of Yeast Protein-disulfide Isomerase in Its Interplay with Endoplasmic Reticulum Oxidoreductin 1.
Coexpression of Kar2p, Pdi1p and Ero1p gave a synergistic effect on CPY expression
Title: Effects of signal sequences and folding accessory proteins on extracellular expression of carboxypeptidase Y in recombinant Saccharomyces cerevisiae.
Pdi1p is the key regulator of Ero1p activity.
Title: Balanced Ero1 activation and inactivation establishes ER redox homeostasis.
analysis of the oxidative activity of yeast Ero1p on protein disulfide isomerase and related oxidoreductases of the endoplasmic reticulum
Title: Oxidative activity of yeast Ero1p on protein disulfide isomerase and related oxidoreductases of the endoplasmic reticulum.
The introduction of PDI improves secretory proteins in Pichia pastoris secretion 4-7 times, which may act synergistically to increase recombinant protein yields.
Title: Enhanced secretion of heterologous proteins in Pichia pastoris following overexpression of Saccharomyces cerevisiae chaperone proteins.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables isomerase activity	IEA Inferred from Electronic Annotation more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein disulfide isomerase activity	IBA Inferred from Biological aspect of Ancestor more info
enables protein disulfide isomerase activity	IDA Inferred from Direct Assay more info	PubMed
enables protein disulfide isomerase activity	IEA Inferred from Electronic Annotation more info
enables protein disulfide isomerase activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables protein-disulfide reductase activity	IDA Inferred from Direct Assay more info	PubMed
enables unfolded protein binding	IDA Inferred from Direct Assay more info	PubMed

Process	Evidence Code	Pubs
involved_in mannose trimming involved in glycoprotein ERAD pathway	IDA Inferred from Direct Assay more info	PubMed
involved_in mannose trimming involved in glycoprotein ERAD pathway	IGI Inferred from Genetic Interaction more info	PubMed
involved_in mannose trimming involved in glycoprotein ERAD pathway	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in protein alpha-1,2-demannosylation	IDA Inferred from Direct Assay more info	PubMed
involved_in protein folding	IBA Inferred from Biological aspect of Ancestor more info
involved_in protein folding	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in response to endoplasmic reticulum stress	IBA Inferred from Biological aspect of Ancestor more info

Component	Evidence Code	Pubs
located_in endoplasmic reticulum	HDA more info	PubMed
is_active_in endoplasmic reticulum	IBA Inferred from Biological aspect of Ancestor more info
located_in endoplasmic reticulum	IEA Inferred from Electronic Annotation more info
located_in endoplasmic reticulum lumen	IDA Inferred from Direct Assay more info	PubMed
located_in endoplasmic reticulum lumen	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: protein disulfide isomerase PDI1

NP_009887.1

EC 5.3.4.1

Protein disulfide isomerase; multifunctional oxidoreductase of the ER lumen, essential for disulfide bond formation in secretory and cell-surface proteins, processing of non-native disulfide bonds; Ero1p activator; complexes with exomannosidase, Mnl1p to facilitate the recognition of misfolded glycoproteins and the trimming of glycan Man8GlcNAc2 to Man7GlcNAc2 on substrates, thereby accelerating ERAD; PDI1 has a paralog, EUG1, that arose from the whole genome duplication

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001135.5 Reference assembly

Range

48653..50221 complement

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001178688.1 → NP_009887.1 TPA: protein disulfide isomerase PDI1 [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_009887.1

Status: REVIEWED

UniProtKB/Swiss-Prot

D6VQX3, P17967

UniProtKB/TrEMBL

B3LU41, N1P7R5

Conserved Domains (1) summary

TIGR01130
Location:32 → 492

ER_PDI_fam; protein disulfide isomerase, eukaryotic