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    PIS1 CDP-diacylglycerol--inositol 3-phosphatidyltransferase [ Saccharomyces cerevisiae S288C ]

    Gene ID: 856229, updated on 1-Aug-2024

    Summary

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    Gene symbol
    PIS1
    Gene description
    CDP-diacylglycerol--inositol 3-phosphatidyltransferase
    Primary source
    FungiDB:YPR113W
    Locus tag
    YPR113W
    See related
    SGD:S000006317; AllianceGenome:SGD:S000006317; VEuPathDB:YPR113W
    Gene type
    protein coding
    RefSeq status
    REVIEWED
    Organism
    Saccharomyces cerevisiae S288C (strain: S288C)
    Lineage
    Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
    Summary
    Enables CDP-diacylglycerol-inositol 3-phosphatidyltransferase activity. Involved in phosphatidylinositol biosynthetic process. Located in Golgi apparatus and mitochondrial outer membrane. Is integral component of membrane. Used to study cancer. Orthologous to human CDIPT (CDP-diacylglycerol--inositol 3-phosphatidyltransferase). [provided by Alliance of Genome Resources, Apr 2022]
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    Genomic context

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    See PIS1 in Genome Data Viewer
    Location:
    chromosome: XVI
    Exon count:
    1
    Sequence:
    Chromosome: XVI; NC_001148.4 (752257..752919)

    Chromosome XVI - NC_001148.4Genomic Context describing neighboring genes Neighboring gene serine/threonine-protein kinase DBF20 Neighboring gene RNA-binding ribosome biosynthesis protein MRD1 Neighboring gene uncharacterized protein Neighboring gene Rgc1p

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_001148.4

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Labeling of Phosphatidylinositol Lipid Products in Cells through Metabolic Engineering by Using a Clickable myo-Inositol Probe. Ricks TJ, et al. Chembiochem, 2019 Jan 18. PMID 30098105, Free PMC Article
    2. The active site of yeast phosphatidylinositol synthase Pis1 is facing the cytosol. Bochud A, et al. Biochim Biophys Acta, 2015 May. PMID 25687304
    3. Regulated transcription of the Saccharomyces cerevisiae phosphatidylinositol biosynthetic gene, PIS1, yields pleiotropic effects on phospholipid synthesis. Jani NM, et al. FEMS Yeast Res, 2009 Jun. PMID 19456874
    4. Expression of the Saccharomyces cerevisiae PIS1 gene is modulated by multiple ATGs in the promoter. Iverson S, et al. Biochem Biophys Res Commun, 2006 Nov 10. PMID 16997274
    5. Molecular cloning, functional complementation in Saccharomyces cerevisiae and enzymatic properties of phosphatidylinositol synthase from the protozoan parasite Toxoplasma gondii. Séron K, et al. Eur J Biochem, 2000 Nov. PMID 11054108

    See all (59) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Cytosolically made inositol and CDP-diacylglycerol can access the active site of the yeast PI synthase Pis1 from the cytosolic side and that Pis1 structure is strongly affected by mild detergents.
      Title: The active site of yeast phosphatidylinositol synthase Pis1 is facing the cytosol.
    2. Ume6p was found to be a positive regulator of PIS1 gene expression.
      Title: Transcription regulation of the Saccharomyces cerevisiae PIS1 gene by inositol and the pleiotropic regulator, Ume6p.
    3. multiple upstream AUG codons are present in at least some PIS1 transcripts and negatively impact PIS1 expression
      Title: Expression of the Saccharomyces cerevisiae PIS1 gene is modulated by multiple ATGs in the promoter.
    4. This study employed a genomic screen, database mining, and more traditional promoter analysis to identify genes that affect PIS1 expression.
      Title: Genomic analysis of PIS1 gene expression.
    5. PIS1-encoded phosphatidylinositol synthase in Saccharomyces cerevisiae is regulated by zinc
      Title: Regulation of the PIS1-encoded phosphatidylinositol synthase in Saccharomyces cerevisiae by zinc.
    6. Enhanced phosphatidylinositol synthesis may account for PIS1 suppression of rsp5 defects.
      Title: Enhanced levels of Pis1p (phosphatidylinositol synthase) improve the growth of Saccharomyces cerevisiae cells deficient in Rsp5 ubiquitin ligase.
    Submit: New GeneRIF Correction

    Pathways from PubChem

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    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Gene Ontology Provided by SGD

    Function Evidence Code Pubs
    enables CDP-diacylglycerol-inositol 3-phosphatidyltransferase activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables CDP-diacylglycerol-inositol 3-phosphatidyltransferase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables CDP-diacylglycerol-inositol 3-phosphatidyltransferase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables CDP-diacylglycerol-inositol 3-phosphatidyltransferase activity IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    enables metal ion binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables phosphotransferase activity, for other substituted phosphate groups IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables transferase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    Process Evidence Code Pubs
    involved_in lipid biosynthetic process IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in phosphatidylinositol biosynthetic process IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in phosphatidylinositol biosynthetic process IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in phosphatidylinositol biosynthetic process IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in phospholipid biosynthetic process IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in phospholipid metabolic process IEA
    Inferred from Electronic Annotation
    more info
    		  
    Component Evidence Code Pubs
    is_active_in Golgi apparatus IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in Golgi apparatus IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in endoplasmic reticulum HDA PubMed 
    located_in endoplasmic reticulum IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in endoplasmic reticulum membrane IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in membrane IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in membrane IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in mitochondrial outer membrane IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in mitochondrial outer membrane IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in mitochondrion HDA PubMed 
    located_in mitochondrion IEA
    Inferred from Electronic Annotation
    more info
    		  

    General protein information

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    Preferred Names
    CDP-diacylglycerol--inositol 3-phosphatidyltransferase
    NP_015438.1
    • Phosphatidylinositol synthase; required for biosynthesis of phosphatidylinositol, which is a precursor for polyphosphoinositides, sphingolipids, and glycolipid anchors for some of the plasma membrane proteins

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_001148.4 Reference assembly

      Range
      752257..752919
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NM_001184210.1 → NP_015438.1  TPA: CDP-diacylglycerol--inositol 3-phosphatidyltransferase [Saccharomyces cerevisiae S288C]

      See identical proteins and their annotated locations for NP_015438.1

      Status: REVIEWED

      UniProtKB/Swiss-Prot
      D6W4B2, P06197
      UniProtKB/TrEMBL
      A6ZWZ4, B3LK77, B5VTQ3, C7GXS5, C8ZJD5, G2WPT0, N1NVL1
      Conserved Domains (1) summary
      COG0558
      Location:6 → 175
      PgsA; Phosphatidylglycerophosphate synthase [Lipid transport and metabolism]
    Nucleotide Protein
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    P06197.1

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