PNO1 Pno1p [Saccharomyces cerevisiae S288C] - Gene

Summary

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Gene symbol: PNO1
Gene description: Pno1p
Primary source: FungiDB:YOR145C
Locus tag: YOR145C
See related: SGD:S000005671; AllianceGenome:SGD:S000005671; VEuPathDB:YOR145C
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as: DIM2; RRP20
Summary: Enables rRNA primary transcript binding activity and unfolded protein binding activity. Involved in proteasome assembly and ribosome biogenesis. Acts upstream of or within ribosomal small subunit export from nucleus. Located in nucleolus. Part of 90S preribosome. Orthologous to human PNO1 (partner of NOB1 homolog). [provided by Alliance of Genome Resources, Apr 2022]
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Genomic context

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See PNO1 in Genome Data Viewer

Location:: chromosome: XV

Exon count:: 1

Sequence:: Chromosome: XV; NC_001147.6 (605347..606171, complement)

Chromosome XV - NC_001147.6 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001147.6

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Findings show how the structurally related Krr1 and Dim2 can control stepwise ribosome assembly during the 90S-to-pre-40S subunit transition.
Title: Interdependent action of KH domain proteins Krr1 and Dim2 drive the 40S platform assembly.
interaction between Dim2 and Nob1 is essential for optimal growth, and RNA binding experiments show that Dim2 increases Nob1 RNA affinity.
Title: Roles of Dim2 in ribosome assembly.

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Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables RNA binding	IEA Inferred from Electronic Annotation more info
enables rRNA primary transcript binding	IDA Inferred from Direct Assay more info	PubMed
enables unfolded protein binding	IDA Inferred from Direct Assay more info	PubMed

Process	Evidence Code	Pubs
involved_in endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA)	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in proteasome assembly	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in proteasome assembly	IPI Inferred from Physical Interaction more info	PubMed
acts_upstream_of_or_within ribosomal small subunit export from nucleus	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in ribosome assembly	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in ribosome biogenesis	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
part_of 90S preribosome	HDA more info	PubMed
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
is_active_in nucleolus	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleolus	IDA Inferred from Direct Assay more info	PubMed
located_in nucleolus	IEA Inferred from Electronic Annotation more info
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: Pno1p

NP_014788.1

Essential nucleolar protein required for pre-18S rRNA processing; interacts with Dim1p, an 18S rRNA dimethyltransferase, and also with Nob1p, which is involved in proteasome biogenesis; contains a KH domain

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001147.6 Reference assembly

Range

605347..606171 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001183564.1 → NP_014788.1 TPA: Pno1p [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_014788.1

Status: REVIEWED

UniProtKB/Swiss-Prot

D6W2K3, Q02462, Q7LHP7, Q99216

UniProtKB/TrEMBL

A0A8H4F7A6, B3LJI3, C7GN78, C8ZGQ3, G2WN18, N1P379

Conserved Domains (1) summary

COG1094
Location:92 → 274

Krr1; rRNA processing protein Krr1/Pno1, contains KH domain [Translation, ribosomal structure and biogenesis]