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    CWC24 [ Saccharomyces cerevisiae S288C ]

    Gene ID: 851033, updated on 11-Sep-2024

    Summary

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    Official Symbol
    CWC24
    Primary source
    SGD:S000004315
    Locus tag
    YLR323C
    See related
    AllianceGenome:SGD:S000004315; FungiDB:YLR323C; VEuPathDB:YLR323C
    Gene type
    protein coding
    RefSeq status
    REVIEWED
    Organism
    Saccharomyces cerevisiae S288C (strain: S288C)
    Lineage
    Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
    Summary
    Predicted to enable DNA binding activity and metal ion binding activity. Involved in generation of catalytic spliceosome for first transesterification step and snoRNA splicing. Located in nucleus. Part of U2-type spliceosomal complex. Human ortholog(s) of this gene implicated in nonphotosensitive trichothiodystrophy 5. Orthologous to human RNF113A (ring finger protein 113A) and RNF113B (ring finger protein 113B). [provided by Alliance of Genome Resources, Apr 2022]
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    Genomic context

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    Location:
    chromosome: XII
    Exon count:
    1
    Sequence:
    Chromosome: XII; NC_001144.5 (778173..778952, complement)

    Chromosome XII - NC_001144.5Genomic Context describing neighboring genes Neighboring gene Mms22p Neighboring gene Sfh1p Neighboring gene peroxisome biogenesis protein Neighboring gene ribosomal 60S subunit protein L38

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_001144.5

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Prp2-mediated protein rearrangements at the catalytic core of the spliceosome as revealed by dcFCCS. Ohrt T, et al. RNA, 2012 Jun. PMID 22535589, Free PMC Article
    2. Functional analysis of Cwc24 ZF-domain in 5' splice site selection. Wu NY, et al. Nucleic Acids Res, 2019 Nov 4. PMID 31504764, Free PMC Article
    3. Cwc24p, a novel Saccharomyces cerevisiae nuclear ring finger protein, affects pre-snoRNA U3 splicing. Goldfeder MB, et al. J Biol Chem, 2008 Feb 1. PMID 17974558
    4. Cwc24p is a general Saccharomyces cerevisiae splicing factor required for the stable U2 snRNP binding to primary transcripts. Coltri PP, et al. PLoS One, 2012. PMID 23029180, Free PMC Article
    5. A protein map of the yeast activated spliceosome as obtained by electron microscopy. Sun C, et al. RNA, 2016 Sep. PMID 27368340, Free PMC Article

    See all (20) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Deletion of the ZF domain or mutation of the conserved ZF residues greatly weakened the association of Cwc24 with the spliceosome, and lowered the affinity and specificity of its interaction with the 5' splice site, resulting in atypical interactions of U5, U6 and Prp8 with the 5' splice site, and aberrant cleavage at the 5' splice site
      Title: Functional analysis of Cwc24 ZF-domain in 5' splice site selection.
    2. propose a model in which Cwc24p is required for stabilizing the U2 association with primary transcripts, and therefore, especially important for splicing of RNAs containing non-consensus branchpoint sequences
      Title: Cwc24p is a general Saccharomyces cerevisiae splicing factor required for the stable U2 snRNP binding to primary transcripts.
    3. Cwc24p localizes to the cell nucleus, and its depletion leads to the accumulation of both U3 pre-snoRNAs.
      Title: Cwc24p, a novel Saccharomyces cerevisiae nuclear ring finger protein, affects pre-snoRNA U3 splicing.
    Submit: New GeneRIF Correction

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Gene Ontology Provided by SGD

    Function Evidence Code Pubs
    enables DNA binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables metal ion binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables zinc ion binding RCA
    inferred from Reviewed Computational Analysis
    more info
    		 PubMed 
    Process Evidence Code Pubs
    involved_in RNA splicing IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in generation of catalytic spliceosome for first transesterification step IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in mRNA processing IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in mRNA splicing, via spliceosome IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in snoRNA splicing IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in snoRNA splicing IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    Component Evidence Code Pubs
    part_of U2-type spliceosomal complex IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    part_of U2-type spliceosomal complex IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in nucleus IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in nucleus IEA
    Inferred from Electronic Annotation
    more info
    		  
    part_of spliceosomal complex IEA
    Inferred from Electronic Annotation
    more info
    		  
    part_of spliceosomal complex IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 

    General protein information

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    Preferred Names
    U2-type spliceosomal complex subunit CWC24
    NP_013427.1
    • General splicing factor; required for stable U2 snRNP binding to primary transcripts; essential for the first step of splicing; component of the pre-catalytic spliceosome complex containing Cef1p; similar to S. pombe Cwf24p

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_001144.5 Reference assembly

      Range
      778173..778952 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NM_001182212.1 → NP_013427.1  TPA: U2-type spliceosomal complex subunit CWC24 [Saccharomyces cerevisiae S288C]

      See identical proteins and their annotated locations for NP_013427.1

      Status: REVIEWED

      UniProtKB/Swiss-Prot
      D6VYW5, P53769
      UniProtKB/TrEMBL
      A7A1K6, B3RHJ8, B5VNL8, C8ZDT4, G2WJE4, N1P745
      Conserved Domains (1) summary
      COG5152
      Location:1 → 259
      COG5152; Uncharacterized conserved protein, contains RING and CCCH-type Zn-fingers [General function prediction only]
    Nucleotide Protein
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    P53769.1

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