Izumo1r IZUMO1 receptor, JUNO [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Izumo1rprovided by MGI
Official Full Name: IZUMO1 receptor, JUNOprovided by MGI
Primary source: MGI:MGI:1929185
See related: Ensembl:ENSMUSG00000031933 AllianceGenome:MGI:1929185
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: Juno; Folr4; Folbp3; 0910001L11Rik
Summary: Enables protein binding activity involved in heterotypic cell-cell adhesion; signaling receptor activity; and signaling receptor binding activity. Involved in cell adhesion; fusion of sperm to egg plasma membrane involved in single fertilization; and sperm-egg recognition. Predicted to be located in membrane. Predicted to be active in external side of plasma membrane. Is expressed in several structures, including alimentary system; endocrine gland; genitourinary system; immune system; and liver and biliary system. Orthologous to human IZUMO1R (IZUMO1 receptor, JUNO). [provided by Alliance of Genome Resources, Oct 2024]
Expression: Biased expression in thymus adult (RPKM 7.0), spleen adult (RPKM 6.0) and 3 other tissues See more
Orthologs: human all
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Genomic context

Location:: 9 A2; 9 4.42 cM

Exon count:: 8

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	9	NC_000075.7 (14797110..14815247, complement)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	9	NC_000075.6 (14885814..14903951, complement)

Chromosome 9 - NC_000075.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Treg cells require Izumo1R to regulate gammadeltaT cell-driven inflammation in the skin.
Title: Treg cells require Izumo1R to regulate γδT cell-driven inflammation in the skin.
Sperm-oocyte signaling: the role of IZUMO1R and CD9 in PTK2B activation and actin remodeling at the sperm binding sitedagger.
Title: Sperm-oocyte signaling: the role of IZUMO1R and CD9 in PTK2B activation and actin remodeling at the sperm binding site†.
Alternative splicing of the Izumo1 gene ensures triggering gamete fusion in mice.
Title: Alternative splicing of the Izumo1 gene ensures triggering gamete fusion in mice.
JUNO interacts with IZUMO1 during sperm binding.Trp62 of JUNO interacts with hydrophobic residues on the alpha-helical core region of IZUMO1.Surface-exposed tryptophan residue of JUNO is required for sperm binding and fertilization.
Title: Structural and functional insights into IZUMO1 recognition by JUNO in mammalian fertilization.
Juno was expressed in oocytes at the germinal vesicle stage and it continues to be expressed at similar levels in later stages of oocytes maturation.
Title: Location and expression of Juno in mice oocytes during maturation.
JUNO associates with monomeric IZUMO1, which is then quickly removed as tight adhesion of the two cells is subsequently established.
Title: Oocyte-triggered dimerization of sperm IZUMO1 promotes sperm-egg fusion in mice.
Binding of sperm protein Izumo1 and its egg receptor Juno drives Cd9 accumulation in the intercellular contact area prior to fusion during mammalian fertilization.
Title: Binding of sperm protein Izumo1 and its egg receptor Juno drives Cd9 accumulation in the intercellular contact area prior to fusion during mammalian fertilization.
Of Juno, neuropeptide release, and light-activated chloride channels. [review]
Title: Of Juno, neuropeptide release, and light-activated chloride channels.
identification of folate receptor 4 (Folr4) as the receptor for Izumo1 on the mouse egg, and propose to rename it Juno; female mice lacking Juno are infertile and Juno-deficient eggs do not fuse with normal sperm
Title: Juno is the egg Izumo receptor and is essential for mammalian fertilization.
Distinct from the expression profile of conventional TFH markers, FR4 was highly expressed by naive CD4(+) T cells, was downregulated after activation and subsequently re-expressed on TFH cells.
Title: Identification of novel markers for mouse CD4(+) T follicular helper cells.

Submit: New GeneRIF Correction See all GeneRIFs (15)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Endonuclease-mediated (2) 1 citation
Targeted (6) 1 citation

General gene information

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Markers

Folr4 (e-PCR), detects polymorphism
- UniSTS:238149

Folr4 (e-PCR), detects polymorphism
- UniSTS:238150

Folr4 (e-PCR), detects polymorphism
- UniSTS:205457

C86255 (e-PCR)
- UniSTS:165616

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
NOT enables folic acid binding	IMP Inferred from Mutant Phenotype more info	PubMed
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein binding involved in heterotypic cell-cell adhesion	IDA Inferred from Direct Assay more info	PubMed
enables signaling receptor activity	IBA Inferred from Biological aspect of Ancestor more info
enables signaling receptor activity	IPI Inferred from Physical Interaction more info	PubMed
enables signaling receptor activity	ISO Inferred from Sequence Orthology more info
enables signaling receptor binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
involved_in cell adhesion	IBA Inferred from Biological aspect of Ancestor more info
involved_in cell adhesion	IDA Inferred from Direct Assay more info	PubMed
involved_in cell adhesion	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in fusion of sperm to egg plasma membrane involved in single fertilization	IBA Inferred from Biological aspect of Ancestor more info
involved_in fusion of sperm to egg plasma membrane involved in single fertilization	IDA Inferred from Direct Assay more info	PubMed
involved_in fusion of sperm to egg plasma membrane involved in single fertilization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in heterotypic cell-cell adhesion	IEA Inferred from Electronic Annotation more info
involved_in single fertilization	IDA Inferred from Direct Assay more info	PubMed
involved_in single fertilization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in sperm-egg recognition	IBA Inferred from Biological aspect of Ancestor more info
involved_in sperm-egg recognition	IDA Inferred from Direct Assay more info	PubMed
involved_in sperm-egg recognition	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
is_active_in external side of plasma membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in membrane	TAS Traceable Author Statement more info	PubMed
located_in plasma membrane	IEA Inferred from Electronic Annotation more info
located_in side of membrane	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: sperm-egg fusion protein Juno

Names: FR-delta; IZUMO1 receptor protein JUNO; folate receptor 4 (delta); folate receptor delta; folate-binding protein 3; probable folate receptor delta

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_022888.3 → NP_075026.1 sperm-egg fusion protein Juno isoform 1 precursor

See identical proteins and their annotated locations for NP_075026.1

Status: VALIDATED

Source sequence(s)

AC136743

Consensus CDS

CCDS22831.1

UniProtKB/Swiss-Prot

B0FFS5, Q9EQF4

Related

ENSMUSP00000034409.8, ENSMUST00000034409.14

Conserved Domains (1) summary

pfam03024
Location:26 → 202

Folate_rec; Folate receptor family
NM_176807.5 → NP_789777.1 sperm-egg fusion protein Juno isoform 2 precursor

See identical proteins and their annotated locations for NP_789777.1

Status: VALIDATED

Source sequence(s)

AC136743

Consensus CDS

CCDS22830.1

UniProtKB/TrEMBL

G5E8D3, Q8BY83

Related

ENSMUSP00000063632.4, ENSMUST00000069408.10

Conserved Domains (1) summary

pfam03024
Location:26 → 155

Folate_rec; Folate receptor family

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000075.7 Reference GRCm39 C57BL/6J

Range

14797110..14815247 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_017313503.2 → XP_017168992.1 sperm-egg fusion protein Juno isoform X5

UniProtKB/TrEMBL

G5E8D3

Conserved Domains (1) summary

pfam03024
Location:34 → 163

Folate_rec; Folate receptor family
XM_017313504.3 → XP_017168993.1 sperm-egg fusion protein Juno isoform X7

UniProtKB/TrEMBL

D3YZI1

Related

ENSMUSP00000113459.2, ENSMUST00000117620.8

Conserved Domains (1) summary

pfam03024
Location:34 → 164

Folate_rec; Folate receptor family
XM_006510530.2 → XP_006510593.1 sperm-egg fusion protein Juno isoform X2

See identical proteins and their annotated locations for XP_006510593.1

UniProtKB/Swiss-Prot

Q9EQF4

Conserved Domains (1) summary

pfam03024
Location:26 → 238

Folate_rec; Folate receptor family
XM_006510531.3 → XP_006510594.1 sperm-egg fusion protein Juno isoform X2

See identical proteins and their annotated locations for XP_006510594.1

UniProtKB/Swiss-Prot

Q9EQF4

Conserved Domains (1) summary

pfam03024
Location:26 → 238

Folate_rec; Folate receptor family
XM_017313501.2 → XP_017168990.1 sperm-egg fusion protein Juno isoform X1

Conserved Domains (1) summary

pfam03024
Location:34 → 246

Folate_rec; Folate receptor family
XM_006510533.3 → XP_006510596.1 sperm-egg fusion protein Juno isoform X4

UniProtKB/TrEMBL

B0FFS7

Conserved Domains (1) summary

pfam03024
Location:64 → 147

Folate_rec; Folate receptor family
XM_017313502.2 → XP_017168991.1 sperm-egg fusion protein Juno isoform X3

Conserved Domains (1) summary

pfam03024
Location:34 → 210

Folate_rec; Folate receptor family
XM_006510536.1 → XP_006510599.1 sperm-egg fusion protein Juno isoform X6

Conserved Domains (1) summary

pfam03024
Location:1 → 127

Folate_rec; Folate receptor family