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    Mgmt O-6-methylguanine-DNA methyltransferase [ Rattus norvegicus (Norway rat) ]

    Gene ID: 25332, updated on 10-Jul-2024

    Summary

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    Official Symbol
    Mgmtprovided by RGD
    Official Full Name
    O-6-methylguanine-DNA methyltransferaseprovided by RGD
    Primary source
    RGD:3087
    See related
    EnsemblRapid:ENSRNOG00000016038 AllianceGenome:RGD:3087
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Rattus norvegicus
    Lineage
    Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus
    Summary
    Enables calcium ion binding activity and methylated-DNA-[protein]-cysteine S-methyltransferase activity. Involved in several processes, including DNA dealkylation involved in DNA repair; cellular response to ionizing radiation; and response to folic acid. Located in nucleus. Biomarker of colon adenocarcinoma and melanoma. Human ortholog(s) of this gene implicated in several diseases, including carcinoma (multiple); endocrine gland cancer (multiple); granulosa cell tumor; nervous system cancer (multiple); and urinary bladder cancer. Orthologous to human MGMT (O-6-methylguanine-DNA methyltransferase). [provided by Alliance of Genome Resources, Apr 2022]
    Expression
    Biased expression in Liver (RPKM 160.5), Spleen (RPKM 119.4) and 9 other tissues See more
    Orthologs
    human mouse all
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    Genomic context

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    See Mgmt in Genome Data Viewer
    Location:
    1q41
    Exon count:
    6
    Annotation release Status Assembly Chr Location
    RS_2024_02 current GRCr8 (GCF_036323735.1) 1 NC_086019.1 (201140832..201367481)
    RS_2023_06 previous assembly mRatBN7.2 (GCF_015227675.2) 1 NC_051336.1 (191710980..191937760)
    106 previous assembly Rnor_6.0 (GCF_000001895.5) 1 NC_005100.4 (209237255..209464189)

    Chromosome 1 - NC_086019.1Genomic Context describing neighboring genes Neighboring gene uncharacterized LOC134485188 Neighboring gene cilia- and flagella-associated protein 251-like Neighboring gene uncharacterized LOC102552565 Neighboring gene EBF transcription factor 3

    Genomic regions, transcripts, and products

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    Go to nucleotide: Graphics FASTA GenBank

    Expression

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    • Project title: A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages
    • Description: 320 RNA samples isolated from 11 organs (adrenal gland, brain, heart, kidney, liver, lung, muscle, spleen, thymus, and testes or uterus) from both sexes of Fischer 344 rats across four developmental stages (2-, 6-, 21-, and 104-weeks-old)
    • BioProject: PRJNA238328
    • Publication: PMID 24510058
    • Analysis date: Mon Jun 6 17:44:12 2016

    Bibliography

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    Related articles in PubMed

    1. MGMT-Mediated neuron Apoptosis in Injured Rat Spinal Cord. Ni Y, et al. Tissue Cell, 2020 Feb. PMID 32433023
    2. Inhibition of poly(ADP-ribose) polymerase enhances the effect of chemotherapy in an animal model of regional therapy for the treatment of advanced extremity malignant melanoma. Toshimitsu H, et al. Ann Surg Oncol, 2010 Aug. PMID 20182810
    3. Discrimination of carcinogens by hepatic transcript profiling in rats following 28-day administration. Matsumoto H, et al. Cancer Inform, 2009 Nov 13. PMID 20011461, Free PMC Article
    4. O6-methylguanine DNA methyltransferase deficiency and response to temozolomide-based therapy in patients with neuroendocrine tumors. Kulke MH, et al. Clin Cancer Res, 2009 Jan 1. PMID 19118063, Free PMC Article
    5. Methylation profiles of multiple CpG island loci in extrahepatic cholangiocarcinoma versus those of intrahepatic cholangiocarcinomas. Kim BH, et al. Arch Pathol Lab Med, 2007 Jun. PMID 17550320

    See all (48) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. MGMT-Mediated neuron Apoptosis in Injured Rat Spinal Cord.
      Title: MGMT-Mediated neuron Apoptosis in Injured Rat Spinal Cord.
    2. Folate deficiency resulted in significant increases in levels of the DNA repair proteins OGG-1 and MGMT in the liver
      Title: Folate deficiency alters hepatic and colon MGMT and OGG-1 DNA repair protein expression in rats but has no effect on genome-wide DNA methylation.
    3. age-related differences in MGMT activity may play a significant role in age-differential susceptibility to rat mammary gland and kidney carcinogenesis
      Title: Methylguanine methyltransferase activity deficiency in immature rat mammary epithelial cells parallels increased carcinogenic susceptibility.
    4. Divalent cations stabilized rat recombinant O6-methylguanine-DNA methyltransferase (rMGMT) protein against heat treatment
      Title: Induction of thermal and chemical stability of O6-methylguanine-DNA methyltransferase by Ca2+.
    Submit: New GeneRIF Correction

    General gene information

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    Markers

    Homology

    Gene Ontology Provided by RGD

    Function Evidence Code Pubs
    enables DNA binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables calcium ion binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables metal ion binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables methylated-DNA-[protein]-cysteine S-methyltransferase activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables methylated-DNA-[protein]-cysteine S-methyltransferase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables methylated-DNA-[protein]-cysteine S-methyltransferase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables methyltransferase activity ISO
    Inferred from Sequence Orthology
    more info
    		  
    Process Evidence Code Pubs
    involved_in DNA alkylation repair IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    acts_upstream_of_or_within DNA alkylation repair ISO
    Inferred from Sequence Orthology
    more info
    		  
    involved_in DNA damage response IEP
    Inferred from Expression Pattern
    more info
    		 PubMed 
    acts_upstream_of_or_within DNA damage response ISO
    Inferred from Sequence Orthology
    more info
    		  
    involved_in DNA repair IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in DNA repair IEA
    Inferred from Electronic Annotation
    more info
    		  
    acts_upstream_of_or_within DNA repair ISO
    Inferred from Sequence Orthology
    more info
    		  
    involved_in cellular response to ionizing radiation IEP
    Inferred from Expression Pattern
    more info
    		 PubMed 
    involved_in cellular response to organic cyclic compound IEP
    Inferred from Expression Pattern
    more info
    		 PubMed 
    involved_in cellular response to oxidative stress IEP
    Inferred from Expression Pattern
    more info
    		 PubMed 
    involved_in mammary gland epithelial cell differentiation IEP
    Inferred from Expression Pattern
    more info
    		 PubMed 
    involved_in methylation IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in negative regulation of apoptotic process IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in negative regulation of apoptotic process ISO
    Inferred from Sequence Orthology
    more info
    		  
    involved_in positive regulation of DNA repair IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in positive regulation of double-strand break repair IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in positive regulation of double-strand break repair ISO
    Inferred from Sequence Orthology
    more info
    		  
    involved_in response to ethanol IEP
    Inferred from Expression Pattern
    more info
    		 PubMed 
    involved_in response to folic acid IEP
    Inferred from Expression Pattern
    more info
    		 PubMed 
    involved_in response to organic cyclic compound IEP
    Inferred from Expression Pattern
    more info
    		 PubMed 
    involved_in response to toxic substance IEP
    Inferred from Expression Pattern
    more info
    		 PubMed 
    involved_in response to xenobiotic stimulus IEP
    Inferred from Expression Pattern
    more info
    		 PubMed 
    Component Evidence Code Pubs
    located_in membrane IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in nucleoplasm IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in nucleoplasm ISO
    Inferred from Sequence Orthology
    more info
    		  
    is_active_in nucleus IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in nucleus IEA
    Inferred from Electronic Annotation
    more info
    		  

    General protein information

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    Preferred Names
    methylated-DNA--protein-cysteine methyltransferase
    Names
    0-6-methylguanine-DNA methyltransferase
    6-O-methylguanine-DNA methyltransferase
    O(6)-alkylguanine-DNA alkyltransferase
    O-6-methylguanine-DNA-alkyltransferase
    O6-alkylguanine-DNA alkyltransferase
    O6-methylguanine-DNA methyltranferase
    NP_036993.1
    XP_038957518.1
    XP_063137786.1

    NCBI Reference Sequences (RefSeq)

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    RefSeqs maintained independently of Annotated Genomes

    These reference sequences exist independently of genome builds. Explain

    These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

    mRNA and Protein(s)

    1. NM_012861.1 → NP_036993.1  methylated-DNA--protein-cysteine methyltransferase

      See identical proteins and their annotated locations for NP_036993.1

      Status: PROVISIONAL

      Source sequence(s)
      S61804
      UniProtKB/Swiss-Prot
      P24528
      UniProtKB/TrEMBL
      A0A8L2QAS3, A6HX75
      Related
      ENSRNOP00000079311.1, ENSRNOT00000119456.2
      Conserved Domains (3) summary
      COG0350
      Location:6 → 178
      AdaB; O6-methylguanine-DNA--protein-cysteine methyltransferase [Replication, recombination and repair]
      TIGR00589
      Location:97 → 177
      ogt; O-6-methylguanine DNA methyltransferase
      pfam02870
      Location:6 → 94
      Methyltransf_1N; 6-O-methylguanine DNA methyltransferase, ribonuclease-like domain

    RefSeqs of Annotated Genomes: GCF_036323735.1-RS_2024_02

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference GRCr8

    Genomic

    1. NC_086019.1 Reference GRCr8

      Range
      201140832..201367481
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. XM_063281716.1 → XP_063137786.1  methylated-DNA--protein-cysteine methyltransferase isoform X2

    2. XM_039101590.2 → XP_038957518.1  methylated-DNA--protein-cysteine methyltransferase isoform X1

      Conserved Domains (2) summary
      TIGR00589
      Location:58 → 138
      ogt; O-6-methylguanine DNA methyltransferase
      pfam02870
      Location:5 → 55
      Methyltransf_1N; 6-O-methylguanine DNA methyltransferase, ribonuclease-like domain
    Nucleotide Protein Strain
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    P24528.3 GenPept UniProtKB/Swiss-Prot:P24528

    Gene LinkOut

    The following LinkOut resources are supplied by external providers. These providers are responsible for maintaining the links.

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