NAP1 histone chaperone NAP1 [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: NAP1
Official Full Name: histone chaperone NAP1
Primary source: SGD:S000001756
Locus tag: YKR048C
See related: AllianceGenome:SGD:S000001756; FungiDB:YKR048C; VEuPathDB:YKR048C
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary: Enables several functions, including cyclin binding activity; histone binding activity; and unfolded protein binding activity. Involved in several processes, including NLS-bearing protein import into nucleus; nucleosome organization; and positive regulation of transcription elongation by RNA polymerase II. Located in cellular bud neck septin collar. Human ortholog(s) of this gene implicated in pancreatic cancer. Orthologous to several human genes including NAP1L1 (nucleosome assembly protein 1 like 1) and NAP1L2 (nucleosome assembly protein 1 like 2). [provided by Alliance of Genome Resources, Nov 2024]
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Genomic context

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See NAP1 in Genome Data Viewer

Location:: chromosome: XI

Exon count:: 1

Sequence:: Chromosome: XI; NC_001143.9 (525387..526640, complement)

Chromosome XI - NC_001143.9 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001143.9

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

an entire reaction scheme for the RSC-mediated nucleosome remodeling process that includes DNA looping, nucleosome ejection, the influence of adjacent nucleosomes, and the coordinated action between Nap1 and RSC.
Title: The synergy between RSC, Nap1 and adjacent nucleosome in nucleosome remodeling.
A Nap1 dimer provides an acidic binding surface and asymmetrically engages a single H2A-H2B heterodimer.
Title: Structural evidence for Nap1-dependent H2A-H2B deposition and nucleosome assembly.
Study found that Nap1 efficiently promotes disassembly of adjacent nucleosomes with which RSC collides and not the disassembly of nucleosomes mobilized by RSC. These results suggests a mechanism by which Nap1 is recruited to actively transcribed regions and assists in the passage of the transcription complex through chromatin, and it provides a novel mechanism for the coordinated action of RSC and Nap1.
Title: Coordinated Action of Nap1 and RSC in Disassembly of Tandem Nucleosomes.
Nap1 is essential for maintaining proper chromatin composition and modulating the exchange of H2A-H2B in vivo.
Title: Histone Chaperone Nap1 Is a Major Regulator of Histone H2A-H2B Dynamics at the Inducible GAL Locus.
these results define the distinct roles for histone chaperones Chz1 and Nap1 to regulate Htz1 incorporation into chromatin.
Title: Distinct roles for histone chaperones in the deposition of Htz1 in chromatin.
Nap1 and DNA directly compete for H2A-H2B histones. Nap1 stabilizes the fold of H2A-H2B histones
Title: Chaperone Nap1 shields histone surfaces used in a nucleosome and can put H2A-H2B in an unconventional tetrameric form.
findings show that Nap1 and Vps75 regulate cryptic transcription observed in the absence of Ctk1. Rtt109 acetylation of H3 K9 also plays a role in this pathway, and both Vps75 and Nap1 promote acetylation of H3 in the ORF.
Title: Histone chaperones Nap1 and Vps75 regulate histone acetylation during transcription elongation.
present evidence for a paradigm in which nucleosome assembly requires the elimination of competing, nonnucleosomal histone-DNA interactions by Nap1.
Title: The histone chaperone Nap1 promotes nucleosome assembly by eliminating nonnucleosomal histone DNA interactions.
the histone chaperones Nap1 and Chz1 have separate Htz1-dependent and -independent functions
Title: Nap1 and Chz1 have separate Htz1 nuclear import and assembly functions.
analysis of a thermodynamic model for Nap1-histone interactions
Title: A thermodynamic model for Nap1-histone interactions.

Submit: New GeneRIF Correction See all GeneRIFs (14)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables DNA binding	IEA Inferred from Electronic Annotation more info
enables chromatin binding	IBA Inferred from Biological aspect of Ancestor more info
enables cyclin binding	IPI Inferred from Physical Interaction more info	PubMed
enables enzyme activator activity	IDA Inferred from Direct Assay more info	PubMed
enables histone binding	IBA Inferred from Biological aspect of Ancestor more info
enables histone binding	IDA Inferred from Direct Assay more info	PubMed
enables unfolded protein binding	IDA Inferred from Direct Assay more info	PubMed

Process	Evidence Code	Pubs
involved_in NLS-bearing protein import into nucleus	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in budding cell bud growth	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in nucleosome assembly	IBA Inferred from Biological aspect of Ancestor more info
involved_in nucleosome assembly	IDA Inferred from Direct Assay more info	PubMed
involved_in nucleosome assembly	IEA Inferred from Electronic Annotation more info
involved_in nucleosome disassembly	IDA Inferred from Direct Assay more info	PubMed
involved_in positive regulation of microtubule polymerization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of transcription elongation by RNA polymerase II	IDA Inferred from Direct Assay more info	PubMed
involved_in protein localization to cell division site after cytokinesis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in ribosomal small subunit biogenesis	IGI Inferred from Genetic Interaction more info	PubMed

Component	Evidence Code	Pubs
located_in cell division site	IDA Inferred from Direct Assay more info	PubMed
located_in cellular bud neck	IEA Inferred from Electronic Annotation more info
located_in cellular bud neck septin collar	IDA Inferred from Direct Assay more info	PubMed
located_in chromatin	IBA Inferred from Biological aspect of Ancestor more info
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleus	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: histone chaperone NAP1

NP_012974.1

Histone chaperone and assembly chaperone for Rps6a/b; involved in histone exchange, replacing histone H2A-H2B dimers or histone variant dimers from assembled nucleosomes; promotes solubility of Rps6a, and 40S ribosomal subunit biogenesis; role in transport of H2A and H2B histones to the nucleus; regulates microtubule dynamics during mitosis; interacts with mitotic cyclin Clb2p; controls bud morphogenesis; phosphorylated by CK2; protein abundance increases in response to DNA replication stress

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001143.9 Reference assembly

Range

525387..526640 complement

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001179838.1 → NP_012974.1 TPA: histone chaperone NAP1 [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_012974.1

Status: REVIEWED

UniProtKB/Swiss-Prot

D6VXA9, P25293, P87196

UniProtKB/TrEMBL

A7A008, G2WI76, N1P0X6

Conserved Domains (1) summary

pfam00956
Location:95 → 362

NAP; Nucleosome assembly protein (NAP)