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    IST1 Ist1p [ Saccharomyces cerevisiae S288C ]

    Gene ID: 855456, updated on 7-Jul-2024

    Summary

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    Gene symbol
    IST1
    Gene description
    Ist1p
    Primary source
    FungiDB:YNL265C
    Locus tag
    YNL265C
    See related
    SGD:S000005209; AllianceGenome:SGD:S000005209; VEuPathDB:YNL265C
    Gene type
    protein coding
    RefSeq status
    REVIEWED
    Organism
    Saccharomyces cerevisiae S288C (strain: S288C)
    Lineage
    Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
    Summary
    Enables ATPase inhibitor activity. Involved in late endosome to vacuole transport via multivesicular body sorting pathway. Located in endosome. Orthologous to human IST1 (IST1 factor associated with ESCRT-III). [provided by Alliance of Genome Resources, Apr 2022]
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    Genomic context

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    See IST1 in Genome Data Viewer
    Location:
    chromosome: XIV
    Exon count:
    2
    Sequence:
    Chromosome: XIV; NC_001146.8 (144280..145281, complement)

    Chromosome XIV - NC_001146.8Genomic Context describing neighboring genes Neighboring gene lysine permease Neighboring gene 1-phosphatidylinositol 4-kinase Neighboring gene phosphatidylinositol transporter Neighboring gene protein transporter YIF1

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_001146.8

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Structural Fine-Tuning of MIT-Interacting Motif 2 (MIM2) and Allosteric Regulation of ESCRT-III by Vps4 in Yeast. Kojima R, et al. J Mol Biol, 2016 Jun 5. PMID 27075672
    2. Recycling of cell surface membrane proteins from yeast endosomes is regulated by ubiquitinated Ist1. Laidlaw KME, et al. J Cell Biol, 2022 Nov 7. PMID 36125415, Free PMC Article
    3. Conformational Changes in the Endosomal Sorting Complex Required for the Transport III Subunit Ist1 Lead to Distinct Modes of ATPase Vps4 Regulation. Tan J, et al. J Biol Chem, 2015 Dec 11. PMID 26515066, Free PMC Article
    4. Ist1 regulates Vps4 localization and assembly. Dimaano C, et al. Mol Biol Cell, 2008 Feb. PMID 18032582, Free PMC Article
    5. Design principles of the ESCRT-III Vps24-Vps2 module. Banjade S, et al. Elife, 2021 May 24. PMID 34028356, Free PMC Article

    See all (37) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Recycling of cell surface membrane proteins from yeast endosomes is regulated by ubiquitinated Ist1.
      Title: Recycling of cell surface membrane proteins from yeast endosomes is regulated by ubiquitinated Ist1.
    2. data support a model in which Ist1-Did2 interactions during ESCRT-III polymerization coordinate Vps4 activity with the timing of ESCRT-III disassembly
      Title: Conformational Changes in the Endosomal Sorting Complex Required for the Transport III Subunit Ist1 Lead to Distinct Modes of ATPase Vps4 Regulation.
    3. Cocrystallization of Ist1NTD with a Did2 fragment shows that Ist1 interacts with the Did2 C-terminal MIM1 (MIT-interacting motif 1) via a novel MIM-binding structural motif.
      Title: Structural basis of Ist1 function and Ist1-Did2 interaction in the multivesicular body pathway and cytokinesis.
    4. A model in which the Ist1-Did2 and Vta1-Vps60 complexes independently modulate late steps in the multivesicular body-sorting pathway, is proposed.
      Title: Novel Ist1-Did2 complex functions at a late step in multivesicular body sorting.
    5. Ist1 has a dual role in the regulation of Vps4 activity.
      Title: Ist1 regulates Vps4 localization and assembly.
    Submit: New GeneRIF Correction

    Pathways from PubChem

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    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Gene Ontology Provided by SGD

    Function Evidence Code Pubs
    enables ATPase inhibitor activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables ATPase inhibitor activity IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    Process Evidence Code Pubs
    involved_in endosome to plasma membrane protein transport IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in late endosome to vacuole transport via multivesicular body sorting pathway IGI
    Inferred from Genetic Interaction
    more info
    		 PubMed 
    involved_in late endosome to vacuole transport via multivesicular body sorting pathway IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in protein localization IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in protein transport IEA
    Inferred from Electronic Annotation
    more info
    		  
    Component Evidence Code Pubs
    located_in cytoplasm IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in cytoplasm IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in endosome HDA PubMed 
    is_active_in endosome IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in endosome IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in endosome IEA
    Inferred from Electronic Annotation
    more info
    		  

    General protein information

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    Preferred Names
    Ist1p
    NP_014134.1
    • Protein involved in recycling of membrane transporters from endosome to plasma membrane; plays a role in the multivesicular body sorting pathway; functions and forms a complex with Did2p; recruitment to endosomes is mediated by the Vps2p-Vps24p subcomplex of ESCRT-III; also interacts with Vps4p

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_001146.8 Reference assembly

      Range
      144280..145281 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NM_001183103.1 → NP_014134.1  TPA: Ist1p [Saccharomyces cerevisiae S288C]

      See identical proteins and their annotated locations for NP_014134.1

      Status: REVIEWED

      UniProtKB/Swiss-Prot
      D6W0S8, P53843
      UniProtKB/TrEMBL
      A6ZRH8, B3LPA5, C7GP97, C8ZFX4, G2WLK0, N1NYD2
      Conserved Domains (1) summary
      pfam03398
      Location:13 → 183
      Ist1; Regulator of Vps4 activity in the MVB pathway
    Nucleotide Protein
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    P53843.1

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