PSMA5 proteasome 20S subunit alpha 5 [Homo sapiens (human)] - Gene

Summary

Official Symbol: PSMA5provided by HGNC
Official Full Name: proteasome 20S subunit alpha 5provided by HGNC
Primary source: HGNC:HGNC:9534
See related: Ensembl:ENSG00000143106 MIM:176844; AllianceGenome:HGNC:9534
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Homo sapiens
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
Also known as: PSC5; ZETA
Summary: The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the peptidase T1A family, that is a 20S core alpha subunit. Multiple alternatively spliced transcript variants encoding two distinct isoforms have been found for this gene. [provided by RefSeq, Dec 2010]
Expression: Ubiquitous expression in lymph node (RPKM 14.6), appendix (RPKM 13.2) and 25 other tissues See more
Orthologs: mouse all
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Genomic context

Location:: 1p13.3

Exon count:: 9

Annotation release	Status	Assembly	Chr	Location
RS_2024_08	current	GRCh38.p14 (GCF_000001405.40)	1	NC_000001.11 (109399042..109426448, complement)
RS_2024_08	current	T2T-CHM13v2.0 (GCF_009914755.1)	1	NC_060925.1 (109431823..109459233, complement)
RS_2024_09	previous assembly	GRCh37.p13 (GCF_000001405.25)	1	NC_000001.10 (109941664..109969070, complement)

Chromosome 1 - NC_000001.11 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: HPA RNA-seq normal tissues HPA RNA-seq normal tissues
Description: RNA-seq was performed of tissue samples from 95 human individuals representing 27 different tissues in order to determine tissue-specificity of all protein-coding genes
BioProject: PRJEB4337
Publication: PMID 24309898
Analysis date: Wed Apr 4 07:08:55 2018

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Knockdown of liver cancer cell-secreted exosomal PSMA5 controls macrophage polarization to restrain cancer progression by blocking JAK2/STAT3 signaling.
Title: Knockdown of liver cancer cell-secreted exosomal PSMA5 controls macrophage polarization to restrain cancer progression by blocking JAK2/STAT3 signaling.
PSMA5 contributes to progression of lung adenocarcinoma in association with the JAK/STAT pathway.
Title: PSMA5 contributes to progression of lung adenocarcinoma in association with the JAK/STAT pathway.
Conserved Mitotic Phosphorylation of a Proteasome Subunit Regulates Cell Proliferation.
Title: Conserved Mitotic Phosphorylation of a Proteasome Subunit Regulates Cell Proliferation.
PSMA5 promotes the tumorigenic process of prostate cancer and is related to bortezomib resistance.
Title: PSMA5 promotes the tumorigenic process of prostate cancer and is related to bortezomib resistance.
increased ubiquitin B mRNA expression followed by significant downregulation of proteasome subunits; 26S proteasome non-ATPase regulatory subunit 1, and proteasome subunit alpha-type 5 was found in pseudoexfoliation syndrome (PEXS) individuals. Decrease in chymotrypsin-like proteasome activity and increased apoptosis were also observed in PEX subjects.
Title: Altered unfolded protein response and proteasome impairment in pseudoexfoliation pathogenesis.
Selective upregulation of the ubiquitin-proteasome proteolytic pathway proteins, proteasome zeta chain and isopeptidase T in fetal Down syndrome.
Title: Selective upregulation of the ubiquitin-proteasome proteolytic pathway proteins, proteasome zeta chain and isopeptidase T in fetal Down syndrome.

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Phenotypes

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Review eQTL and phenotype association data in this region using PheGenI

EBI GWAS Catalog

Description
New loci associated with kidney function and chronic kidney disease. EBI GWAS Catalog EBI GWAS CatalogPubMed

Variation

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See variants in ClinVar

See studies and variants in dbVar

See Variation Viewer (GRCh37.p13)

See Variation Viewer (GRCh38)

HIV-1 interactions

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Replication interactions

Interaction	Pubs
Knockdown of proteasome (prosome, macropain) subunit, alpha type, 5 (PSMA5) by siRNA inhibits the early stages of HIV-1 replication in 293T cells infected with VSV-G pseudotyped HIV-1	PubMed

Protein interactions

Protein	Gene	Interaction	Pubs
Tat	tat	HIV-1 Tat slightly enhances the activity of the purified 26 S proteasome	PubMed
	tat	Amino acids Lys51, Arg52, and Asp67 of HIV-1 Tat represent the proteasome binding site of Tat, and Tat amino acids 37-72 are necessary for proteasomal interaction and suppression of 11 S regulator-mediated antigen presentation	PubMed
	tat	HIV-1 Tat inhibits the peptidase activity of the 20 S proteasome and interferes with the formation of the 20 S proteasome-11 S regulator complex	PubMed
	tat	HIV-1 Tat binds to the alpha2, alpha4, alpha6, alpha7, beta1, beta2, beta3, beta5, beta6, beta7, LMP7/beta5i, and MECL1/beta2i subunits of the proteasome 20 S core structure and can inhibit cellular proteasome function	PubMed
Vif	vif	HIV-1 Vif binds to the cellular cytidine deaminase APOBEC3G and targets it for degradation through an interaction with the proteasome, thereby inhibiting APOBEC3G mediated restriction of HIV-1 replication	PubMed
integrase	gag-pol	Proteasomal degradation of HIV-1 integrase in mammalian cells occurs by the N-end rule pathway	PubMed

Go to the HIV-1, Human Interaction Database

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

NoName (e-PCR)
- UniSTS:489730

D1S1985E (e-PCR)
- UniSTS:151310

G32685 (e-PCR)
- UniSTS:117267

WI-14018 (e-PCR)
- UniSTS:10538

SHGC-75262 (e-PCR)
- UniSTS:56380

SHGC-80868 (e-PCR)
- UniSTS:102515

A009N30 (e-PCR)
- UniSTS:36993

SHGC-75266 (e-PCR)
- UniSTS:47056

RH122125 (e-PCR)
- UniSTS:134877

RH64702 (e-PCR)
- UniSTS:88050

Clone Names

FLJ42315, MGC117302, MGC125802, MGC125803, MGC125804

Gene Ontology Provided by GOA

Function	Evidence Code	Pubs
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
involved_in proteasome-mediated ubiquitin-dependent protein catabolic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in proteasome-mediated ubiquitin-dependent protein catabolic process	NAS Non-traceable Author Statement more info	PubMed

Component	Evidence Code	Pubs
is_active_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
located_in cytosol	IDA Inferred from Direct Assay more info
located_in cytosol	TAS Traceable Author Statement more info
located_in extracellular exosome	HDA more info	PubMed
located_in extracellular region	TAS Traceable Author Statement more info
located_in ficolin-1-rich granule lumen	TAS Traceable Author Statement more info
located_in nucleoplasm	TAS Traceable Author Statement more info
located_in nucleus	HDA more info	PubMed
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
is_active_in nucleus	IDA Inferred from Direct Assay more info	PubMed
part_of proteasome complex	IDA Inferred from Direct Assay more info	PubMed
part_of proteasome complex	NAS Non-traceable Author Statement more info	PubMed
part_of proteasome core complex	IDA Inferred from Direct Assay more info	PubMed
part_of proteasome core complex	ISS Inferred from Sequence or Structural Similarity more info
part_of proteasome core complex, alpha-subunit complex	IBA Inferred from Biological aspect of Ancestor more info
part_of proteasome core complex, alpha-subunit complex	IDA Inferred from Direct Assay more info	PubMed
part_of proteasome core complex, alpha-subunit complex	ISS Inferred from Sequence or Structural Similarity more info
located_in secretory granule lumen	TAS Traceable Author Statement more info

General protein information

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Preferred Names: proteasome subunit alpha type-5

Names: alpha-5; epididymis tissue sperm binding protein Li 11n; macropain subunit zeta; macropain zeta chain; multicatalytic endopeptidase complex zeta chain; proteasome (prosome, macropain) subunit, alpha type, 5; proteasome alpha 5 subunit; proteasome component 5; proteasome subunit alpha 5; proteasome subunit zeta; proteasome zeta chain

NP_001186701.1

EC 3.4.25.1

NP_001186702.1

EC 3.4.25.1

NP_001186703.1

EC 3.4.25.1

NP_002781.2

EC 3.4.25.1

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001199772.2 → NP_001186701.1 proteasome subunit alpha type-5 isoform 2

See identical proteins and their annotated locations for NP_001186701.1

Status: REVIEWED

Description

Transcript Variant: This variant (2) lacks an exon in the 5' region, resulting in a downstream AUG start codon, as compared to variant 1. The resulting isoform (2) is shorter at the N-terminus, as compared to isoform 1.

Source sequence(s)

AK304448, AL390252, DC324498, H08369

Consensus CDS

CCDS55619.1

UniProtKB/Swiss-Prot

P28066

Conserved Domains (1) summary

cl00467
Location:1 → 162

Ntn_hydrolase; The Ntn hydrolases (N-terminal nucleophile) are a diverse superfamily of of enzymes that are activated autocatalytically via an N-terminally lcated nucleophilic amino acid. N-terminal nucleophile (NTN-) hydrolase superfamily, which contains a ...
NM_001199773.2 → NP_001186702.1 proteasome subunit alpha type-5 isoform 2

See identical proteins and their annotated locations for NP_001186702.1

Status: REVIEWED

Description

Transcript Variant: This variant (3) has an alternate 5' UTR exon, resulting in a downstream AUG start codon, as compared to variant 1. The resulting isoform (2) is shorter at the N-terminus, as compared to isoform 1.

Source sequence(s)

AL390252, BI825400, H08369

Consensus CDS

CCDS55619.1

UniProtKB/Swiss-Prot

P28066

Conserved Domains (1) summary

cl00467
Location:1 → 162

Ntn_hydrolase; The Ntn hydrolases (N-terminal nucleophile) are a diverse superfamily of of enzymes that are activated autocatalytically via an N-terminally lcated nucleophilic amino acid. N-terminal nucleophile (NTN-) hydrolase superfamily, which contains a ...
NM_001199774.2 → NP_001186703.1 proteasome subunit alpha type-5 isoform 2

See identical proteins and their annotated locations for NP_001186703.1

Status: REVIEWED

Description

Transcript Variant: This variant (4) has an alternate 5' UTR exon, resulting in a downstream AUG start codon, as compared to variant 1. The resulting isoform (2) is shorter at the N-terminus, as compared to isoform 1.

Source sequence(s)

AL390252, BM793388, DC324021, H08369

Consensus CDS

CCDS55619.1

UniProtKB/Swiss-Prot

P28066

Related

ENSP00000440618.1, ENST00000538610.5

Conserved Domains (1) summary

cl00467
Location:1 → 162

Ntn_hydrolase; The Ntn hydrolases (N-terminal nucleophile) are a diverse superfamily of of enzymes that are activated autocatalytically via an N-terminally lcated nucleophilic amino acid. N-terminal nucleophile (NTN-) hydrolase superfamily, which contains a ...
NM_002790.4 → NP_002781.2 proteasome subunit alpha type-5 isoform 1

See identical proteins and their annotated locations for NP_002781.2

Status: REVIEWED

Description

Transcript Variant: This variant (1) is the predominant transcript and encodes the longer isoform (1).

Source sequence(s)

AK225990, AK313351, AL390252, H08369

Consensus CDS

CCDS799.1

UniProtKB/Swiss-Prot

B2R8F6, B4E2V4, P28066, Q3T1C1, Q6IBF7

UniProtKB/TrEMBL

A0A109NGN6, Q5U0A0

Related

ENSP00000271308.4, ENST00000271308.9

Conserved Domains (1) summary

cd03753
Location:8 → 220

proteasome_alpha_type_5; The 20S proteasome, multisubunit proteolytic complex, is the central enzyme of nonlysosomal protein degradation in both the cytosol and nucleus. It is composed of 28 subunits arranged as four homoheptameric rings that stack on top of one another forming ...