NPR3 Npr3p [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: NPR3
Official Full Name: Npr3p
Primary source: SGD:S000001015
Locus tag: YHL023C
See related: AllianceGenome:SGD:S000001015; FungiDB:YHL023C; VEuPathDB:YHL023C
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as: RMD11
Summary: Involved in several processes, including negative regulation of intracellular signal transduction; positive regulation of cellular response to amino acid starvation; and pseudohyphal growth. Is extrinsic component of fungal-type vacuolar membrane. Part of GATOR1 complex. Used to study cancer. Human ortholog(s) of this gene implicated in focal epilepsy. Orthologous to human NPRL3 (NPR3 like, GATOR1 complex subunit). [provided by Alliance of Genome Resources, Apr 2022]
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Genomic context

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See NPR3 in Genome Data Viewer

Location:: chromosome: VIII

Exon count:: 1

Sequence:: Chromosome: VIII; NC_001140.6 (59123..62563, complement)

Chromosome VIII - NC_001140.6 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001140.6

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Npr2-Npr3-dependent GTP hydrolysis of Gtr1 and direct binding of Gtr2 to Kog1, are involved in TORC1 inactivation and autophagic induction
Title: Reciprocal conversion of Gtr1 and Gtr2 nucleotide-binding states by Npr2-Npr3 inactivates TORC1 and induces autophagy.
A visual screen uncovered three regulators of autophagy-Iml1p, Npr2p, and Npr3p-which function in the same complex and are selectively required for non-nitrogen starvation-induced autophagy
Title: Selective regulation of autophagy by the Iml1-Npr2-Npr3 complex in the absence of nitrogen starvation.
Npr2/3 complex mediates an amino acid starvation signal to TORC1.
Title: A genome-wide screen for regulators of TORC1 in response to amino acid starvation reveals a conserved Npr2/3 complex.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables molecular_function	ND No biological Data available more info

Process	Evidence Code	Pubs
involved_in TORC1 signaling	IBA Inferred from Biological aspect of Ancestor more info
involved_in cellular response to amino acid starvation	IBA Inferred from Biological aspect of Ancestor more info
involved_in cellular response to amino acid starvation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in cellular response to nitrogen starvation	IGI Inferred from Genetic Interaction more info	PubMed
involved_in cellular response to nitrogen starvation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in meiotic cell cycle	IEA Inferred from Electronic Annotation more info
involved_in meiotic cell cycle	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of TOR signaling	IEA Inferred from Electronic Annotation more info
involved_in negative regulation of TORC1 signaling	IBA Inferred from Biological aspect of Ancestor more info
involved_in negative regulation of TORC1 signaling	IGI Inferred from Genetic Interaction more info	PubMed
involved_in negative regulation of TORC1 signaling	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of small GTPase mediated signal transduction	IGI Inferred from Genetic Interaction more info	PubMed
involved_in positive regulation of autophagy	IBA Inferred from Biological aspect of Ancestor more info
involved_in positive regulation of autophagy	IGI Inferred from Genetic Interaction more info	PubMed
involved_in positive regulation of autophagy	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in protein transport	IEA Inferred from Electronic Annotation more info
involved_in pseudohyphal growth	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of autophagosome assembly	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
part_of GATOR1 complex	IBA Inferred from Biological aspect of Ancestor more info
part_of GATOR1 complex	IDA Inferred from Direct Assay more info	PubMed
part_of Seh1-associated complex	IDA Inferred from Direct Assay more info	PubMed
located_in membrane	IEA Inferred from Electronic Annotation more info
located_in vacuolar membrane	IEA Inferred from Electronic Annotation more info
located_in vacuole	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: Npr3p

NP_011840.1

Subunit of the Iml1p/SEACIT complex; SEACIT (Iml1p-Npr2p-Npr3p) is a subcomplex of SEAC, a coatomer-related complex that associates dynamically with the vacuole; Npr3p may have a structural or regulatory role, supporting Iml1p function as a GAP for the Rag family GTPase Gtr1p, and leading to inhibition of TORC1 signaling in response to amino acid deprivation; SEACIT is required for non-nitrogen-starvation-induced autophagy; null mutant has meiotic defects; human NPRL3 homolog

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001140.6 Reference assembly

Range

59123..62563 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001179103.1 → NP_011840.1 TPA: Npr3p [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_011840.1

Status: REVIEWED

UniProtKB/Swiss-Prot

D3DKU5, P38742

UniProtKB/TrEMBL

A6ZSM8, G2WF43, N1P2V9

Conserved Domains (1) summary

pfam03666
Location:400 → 966

NPR3; Nitrogen Permease regulator of amino acid transport activity 3