GPD1 glycerol-3-phosphate dehydrogenase (NAD(+)) GPD1 [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: GPD1
Official Full Name: glycerol-3-phosphate dehydrogenase (NAD(+)) GPD1
Primary source: SGD:S000002180
Locus tag: YDL022W
See related: AllianceGenome:SGD:S000002180; FungiDB:YDL022W; VEuPathDB:YDL022W
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as: DAR1; HOR1; OSG1; OSR5
Summary: Enables glycerol-3-phosphate dehydrogenase [NAD+] activity. Involved in NADH oxidation; intracellular accumulation of glycerol; and protein import into peroxisome matrix. Located in cytosol; nucleus; and peroxisome. Human ortholog(s) of this gene implicated in Brugada syndrome 2. Orthologous to human GPD1 (glycerol-3-phosphate dehydrogenase 1) and GPD1L (glycerol-3-phosphate dehydrogenase 1 like). [provided by Alliance of Genome Resources, Apr 2022]
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Genomic context

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See GPD1 in Genome Data Viewer

Location:: chromosome: IV

Exon count:: 1

Sequence:: Chromosome: IV; NC_001136.10 (411825..413000)

Chromosome IV - NC_001136.10 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001136.10

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Lysine245 plays a crucial role in stability and function of glycerol 3-phosphate dehydrogenase (Gpd1) in Saccharomyces cerevisiae.
Title: Lysine245 plays a crucial role in stability and function of glycerol 3-phosphate dehydrogenase (Gpd1) in Saccharomyces cerevisiae.
High expression of SIP18 at the beginning of rehydration was shown to be due to high transcription of the gene during the drying process.
Title: Expression of GPD1 and SIP18 genes during rehydration in active dry industrial Saccharomyces cerevisiae cider-making yeast strains (ADY).
PUB1 mutant shows increased glycerol-3-phosphate dehydrogenase activity during wine making, which is related with an increase in the Gpd1 levels.
Title: RNA binding protein Pub1p regulates glycerol production and stress tolerance by controlling Gpd1p activity during winemaking.
Stress exposure results in increased peroxisomal levels of yeast Pnc1 and Gpd1, which are imported via a piggy-backing mechanism.
Title: Stress exposure results in increased peroxisomal levels of yeast Pnc1 and Gpd1, which are imported via a piggy-backing mechanism.
Loss of function of the vacuolar H-ATPase (vma1) or a defect in the biosynthesis of the osmolyte glycerol (gpd1) caused a prolonged repression of housekeeping genes and a delay in gene activation and adaptation towards salt stress.
Title: Coordinated gene regulation in the initial phase of salt stress adaptation.
osmotic up-regulation of only two Hog1-dependent glycerol biosynthesis genes, GPD1 and GPP2, is sufficient for successful osmoadaptation.
Title: Rewiring yeast osmostress signalling through the MAPK network reveals essential and non-essential roles of Hog1 in osmoadaptation.
GPD1 silencing gave 20 % less glycerol-3-phosphate dehydrogenase activity, 19 % lower glycerol production, and 9.7 % higher ethanol production compared with the original strain.
Title: Silencing the glycerol-3-phosphate dehydrogenase gene in Saccharomyces cerevisiae results in more ethanol being produced and less glycerol.
The crystal structure of a glycerol-3-phosphate dehydrogenase, GPD1, from S. cerevisiae.
Title: Structure of glycerol-3-phosphate dehydrogenase (GPD1) from Saccharomyces cerevisiae at 2.45 Å resolution.
Phosphorylation of Gpd1 and Gpd2 by distinct kinases enables rapid adaptation to specific stress conditions in Saccharomyces cerevisiae.
Title: Reciprocal phosphorylation of yeast glycerol-3-phosphate dehydrogenases in adaptation to distinct types of stress.

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Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables NAD binding	IEA Inferred from Electronic Annotation more info
enables glycerol-3-phosphate dehydrogenase (NAD+) activity	IEA Inferred from Electronic Annotation more info
enables glycerol-3-phosphate dehydrogenase [NAD(P)+] activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables oxidoreductase activity	IEA Inferred from Electronic Annotation more info
enables oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor	IEA Inferred from Electronic Annotation more info
enables protein homodimerization activity	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in NADH oxidation	IBA Inferred from Biological aspect of Ancestor more info
involved_in NADH oxidation	IEA Inferred from Electronic Annotation more info
involved_in NADH oxidation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in carbohydrate metabolic process	IEA Inferred from Electronic Annotation more info
involved_in glycerol-3-phosphate catabolic process	IEA Inferred from Electronic Annotation more info
involved_in glycerol-3-phosphate metabolic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in intracellular accumulation of glycerol	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in protein import into peroxisome matrix	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
is_active_in cytosol	IBA Inferred from Biological aspect of Ancestor more info
located_in cytosol	IDA Inferred from Direct Assay more info	PubMed
located_in mitochondrion	HDA more info	PubMed
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed
located_in peroxisome	IDA Inferred from Direct Assay more info	PubMed
located_in peroxisome	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: glycerol-3-phosphate dehydrogenase (NAD(+)) GPD1

NP_010262.1

EC 1.1.1.8

NAD-dependent glycerol-3-phosphate dehydrogenase; key enzyme of glycerol synthesis, essential for growth under osmotic stress; expression regulated by high-osmolarity glycerol response pathway; protein abundance increases in response to DNA replication stress; constitutively inactivated via phosphorylation by the protein kinases Ypk1p and Ypk2p, dephosphorylation increases catalytic activity; forms a heterodimer with Pnc1p to facilitate its peroxisomal import

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001136.10 Reference assembly

Range

411825..413000

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001180081.1 → NP_010262.1 TPA: glycerol-3-phosphate dehydrogenase (NAD(+)) GPD1 [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_010262.1

Status: REVIEWED

UniProtKB/Swiss-Prot

A5YWB0, D6VRW8, Q00055, Q6J5J2, Q6J5J5

UniProtKB/TrEMBL

A6ZXU7, B5VFP2, C7GW57, C8Z4P8, G2WCD3, N1P674

Conserved Domains (1) summary

TIGR03376
Location:36 → 383

glycerol3P_DH; glycerol-3-phosphate dehydrogenase (NAD(+))