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    SKP1 [ Saccharomyces cerevisiae S288C ]

    Gene ID: 851928, updated on 11-Sep-2024

    Summary

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    Official Symbol
    SKP1
    Primary source
    SGD:S000002736
    Locus tag
    YDR328C
    See related
    AllianceGenome:SGD:S000002736; FungiDB:YDR328C; VEuPathDB:YDR328C
    Gene type
    protein coding
    RefSeq status
    REVIEWED
    Organism
    Saccharomyces cerevisiae S288C (strain: S288C)
    Lineage
    Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
    Also known as
    MGO1
    Summary
    Enables DNA replication origin binding activity. Contributes to ubiquitin protein ligase activity. Involved in several processes, including cellular protein metabolic process; mitotic cell cycle phase transition; and non-membrane-bounded organelle assembly. Located in cytoplasm and nucleus. Part of CBF3 complex; RAVE complex; and SCF ubiquitin ligase complex. Orthologous to human SKP1 (S-phase kinase associated protein 1). [provided by Alliance of Genome Resources, Apr 2022]
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    Genomic context

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    Location:
    chromosome: IV
    Exon count:
    1
    Sequence:
    Chromosome: IV; NC_001136.10 (1125434..1126018, complement)

    Chromosome IV - NC_001136.10Genomic Context describing neighboring genes Neighboring gene condensin subunit YCG1 Neighboring gene Ysp2p Neighboring gene Pex3p Neighboring gene Ubx5p

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_001136.10

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Pollen-specific SKP1-like proteins are components of functional scf complexes and essential for lily pollen tube elongation. Chang LC, et al. Plant Cell Physiol, 2009 Aug. PMID 19578169
    2. Cloning of a centromere binding factor 3d (CBF3D) gene from Candida glabrata. Stoyan T, et al. Yeast, 1999 Jun 30. PMID 10398347
    3. The crystal structure of the Sgt1-Skp1 complex: the link between Hsp90 and both SCF E3 ubiquitin ligases and kinetochores. Willhoft O, et al. Sci Rep, 2017 Jan 31. PMID 28139700, Free PMC Article
    4. Overexpression of the novel F-box protein Ymr258c confers resistance to methylmercury in Saccharomyces cerevisiae. Hwang GW, et al. J Toxicol Sci, 2009 Oct. PMID 19652464
    5. Sgt1 dimerization is required for yeast kinetochore assembly. Bansal PK, et al. J Biol Chem, 2009 Feb 6. PMID 19073600, Free PMC Article

    See all (175) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. findings revealed that Rok1 protein synthesis is repressed by Psk2 and Skp1; this repression appeared to be mediated through the ROK1 uORF1
      Title: Identification of Psk2, Skp1, and Tub4 as trans-acting factors for uORF-containing ROK1 mRNA in Saccharomyces cerevisiae.
    2. This ubiquitylation and degradation pathway acts on Met30 that is dissociated from Skp1. Unexpectedly, this pathway required the cullin component Cdc53/Cul1 but was independent of the other central SCF component Skp1
      Title: Skp1 Independent Function of Cdc53/Cul1 in F-box Protein Homeostasis.
    3. Saf1p is being a bona fide Skp1-Cullin-F-box subunit
      Title: Skp1-Cullin-F-box-dependent degradation of Aah1p requires its interaction with the F-box protein Saf1p.
    4. Data show that the amino terminus of Sgt1p interacts with CBF3 subunits Skp1p and Ctf13p.
      Title: The interaction between Sgt1p and Skp1p is regulated by HSP90 chaperones and is required for proper CBF3 assembly.
    5. Sgt1p acts as a "client adaptor" recruiting specific clients to Hsp82p co-chaperone complexes
      Title: Sgt1p is a unique co-chaperone that acts as a client adaptor to link Hsp90 to Skp1p.
    6. findings suggest a novel function of Skp1 in mitotic exit and cytokinesis, independent of its function as a part of the SCF complex; interaction of Skp1 and Bfa1 may contribute to the function of Skp1 in the mitotic exit
      Title: A new function of Skp1 in the mitotic exit of budding yeast Saccharomyces cerevisiae.
    7. Skp1p does not appear to be involved in V-ATPase assembly but instead in the cycling of the complex off membranes
      Title: Skp1p regulates Soi3p/Rav1p association with endosomal membranes but is not required for vacuolar ATPase assembly.
    8. the SCFCdc4 dimer accommodates multiple geometries for substrate ubiquitination
      Title: Suprafacial orientation of the SCFCdc4 dimer accommodates multiple geometries for substrate ubiquitination.
    Submit: New GeneRIF Correction

    Pathways from PubChem

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    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Gene Ontology Provided by SGD

    Function Evidence Code Pubs
    enables DNA binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables DNA replication origin binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables cullin family protein binding IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    contributes_to ubiquitin protein ligase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    contributes_to ubiquitin-protein transferase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    Process Evidence Code Pubs
    involved_in G1/S transition of mitotic cell cycle IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in G2/M transition of mitotic cell cycle IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in SCF-dependent proteasomal ubiquitin-dependent protein catabolic process IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in SCF-dependent proteasomal ubiquitin-dependent protein catabolic process IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in exit from mitosis IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in kinetochore assembly IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in kinetochore assembly IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in mitotic cell cycle IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in negative regulation of cytoplasmic translation IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in protein neddylation IGI
    Inferred from Genetic Interaction
    more info
    		 PubMed 
    involved_in protein neddylation IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in protein ubiquitination IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in protein-containing complex assembly IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in regulation of exit from mitosis IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in regulation of exit from mitosis IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    involved_in regulation of protein-containing complex assembly IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    involved_in septin ring assembly IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in ubiquitin-dependent protein catabolic process IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in ubiquitin-dependent protein catabolic process IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in vacuolar acidification IGI
    Inferred from Genetic Interaction
    more info
    		 PubMed 
    involved_in vacuolar acidification IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    Component Evidence Code Pubs
    part_of CBF3 complex IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    part_of CBF3 complex IGI
    Inferred from Genetic Interaction
    more info
    		 PubMed 
    part_of CBF3 complex IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    part_of RAVE complex IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    part_of SCF ubiquitin ligase complex IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    part_of SCF ubiquitin ligase complex IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    located_in chromosome IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in chromosome, centromeric region IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in chromosome, telomeric region IEA
    Inferred from Electronic Annotation
    more info
    		  
    is_active_in cytoplasm IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in cytoplasm IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in cytoplasm IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in kinetochore IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in kinetochore TAS
    Traceable Author Statement
    more info
    		 PubMed 
    is_active_in nucleus IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in nucleus IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in nucleus IEA
    Inferred from Electronic Annotation
    more info
    		  

    General protein information

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    Preferred Names
    SCF ubiquitin ligase subunit SKP1
    NP_010615.3
    • Evolutionarily conserved kinetochore protein; part of multiple protein complexes, including the SCF ubiquitin ligase complex, the CBF3 complex that binds centromeric DNA, and the RAVE complex that regulates assembly of the V-ATPase; protein abundance increases in response to DNA replication stress

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_001136.10 Reference assembly

      Range
      1125434..1126018 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NM_001180636.3 → NP_010615.3  TPA: SCF ubiquitin ligase subunit SKP1 [Saccharomyces cerevisiae S288C]

      See identical proteins and their annotated locations for NP_010615.3

      Status: REVIEWED

      UniProtKB/Swiss-Prot
      D6VSW0, P52286, Q07186
      UniProtKB/TrEMBL
      A6ZYT0, B3LFW9, B5VGL0, C7GWM5, C8Z5P1, G2WB67, N1P6Y0
      Conserved Domains (1) summary
      COG5201
      Location:3 → 194
      SKP1; SCF ubiquitin ligase, SKP1 component [Posttranslational modification, protein turnover, chaperones]
    Nucleotide Protein
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    P52286.2

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