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    IRC3 double-stranded DNA-dependent ATPase [ Saccharomyces cerevisiae S288C ]

    Gene ID: 851932, updated on 17-May-2024

    Summary

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    Gene symbol
    IRC3
    Gene description
    double-stranded DNA-dependent ATPase
    Primary source
    FungiDB:YDR332W
    Locus tag
    YDR332W
    See related
    SGD:S000002740; AllianceGenome:SGD:S000002740; VEuPathDB:YDR332W
    Gene type
    protein coding
    RefSeq status
    REVIEWED
    Organism
    Saccharomyces cerevisiae S288C (strain: S288C)
    Lineage
    Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
    Summary
    Enables Y-form DNA binding activity; double-stranded DNA helicase activity; and forked DNA-dependent helicase activity. Involved in mitochondrial DNA metabolic process. Located in mitochondrial matrix. [provided by Alliance of Genome Resources, Apr 2022]
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    Genomic context

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    See IRC3 in Genome Data Viewer
    Location:
    chromosome: IV
    Exon count:
    1
    Sequence:
    Chromosome: IV; NC_001136.10 (1131001..1133070)

    Chromosome IV - NC_001136.10Genomic Context describing neighboring genes Neighboring gene Ubx5p Neighboring gene GPI-anchor transamidase Neighboring gene Rqc1p Neighboring gene chromatin-remodeling protein SWR1

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_001136.10

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. IRC3 Regulates Mitochondrial Translation in Response to Metabolic Cues in Saccharomyces cerevisiae. Kaur J, et al. Mol Cell Biol, 2021 Oct 26. PMID 34398681, Free PMC Article
    2. Irc3 is a monomeric DNA branch point-binding helicase in mitochondria of the yeast Saccharomyces cerevisiae. Piljukov VJ, et al. FEBS Lett, 2020 Oct. PMID 32735705
    3. Mitochondrial helicase Irc3 translocates along double-stranded DNA. Sedman T, et al. FEBS Lett, 2017 Dec. PMID 29113022
    4. Irc3 is a mitochondrial DNA branch migration enzyme. Gaidutšik I, et al. Sci Rep, 2016 May 19. PMID 27194389, Free PMC Article
    5. Double-stranded DNA-dependent ATPase Irc3p is directly involved in mitochondrial genome maintenance. Sedman T, et al. Nucleic Acids Res, 2014 Dec 1. PMID 25389272, Free PMC Article

    See all (35) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Study revealed that Irc3 - a dsDNA dependent ATPase of the Superfamily II- is essential for yeast mtDNA maintenance. irc3Delta mutant yeast cells accumulated double-stranded breaks in mtDNA and lose the wild-type mitochondrial genome in the course of a few generations of growth on glucose-containing media. These results demonstrate that Irc3 is a mitochondrial branch migration enzyme.
      Title: Irc3 is a mitochondrial DNA branch migration enzyme.
    2. Irc3 disrupts partially triple-stranded DNA structures in an ATP-dependent manner. Our kinetic experiments indicate that the rate of ATP hydrolysis by Irc3 is dependent on the length of the double-stranded DNA cosubstrate.
      Title: Mitochondrial helicase Irc3 translocates along double-stranded DNA.
    3. The data suggest that Irc3p is required to avoid the accumulation of dsDNA breaks that could irreversibly damage the mitochondrial genetic system.
      Title: Double-stranded DNA-dependent ATPase Irc3p is directly involved in mitochondrial genome maintenance.
    Submit: New GeneRIF Correction

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Gene Ontology Provided by SGD

    Function Evidence Code Pubs
    enables ATP binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables DNA binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables Y-form DNA binding IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables Y-form DNA binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables double-stranded DNA helicase activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables double-stranded DNA helicase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables forked DNA-dependent helicase activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables forked DNA-dependent helicase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables helicase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables hydrolase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    Process Evidence Code Pubs
    involved_in DNA duplex unwinding IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in mitochondrial DNA metabolic process IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in mitochondrial DNA metabolic process IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in mitochondrial translational elongation IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    acts_upstream_of_or_within mitochondrial translational elongation IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    Component Evidence Code Pubs
    is_active_in mitochondrial matrix IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in mitochondrial matrix IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in mitochondrion HDA PubMed 
    located_in mitochondrion IEA
    Inferred from Electronic Annotation
    more info
    		  

    General protein information

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    Preferred Names
    double-stranded DNA-dependent ATPase
    NP_010619.3
    • Double-stranded DNA-dependent helicase of the DExH/D-box family; branch point-binding helicase that preferentially unwinds the nascent lagging strand with a 3' to 5' polarity at a replication fork; contains double-stranded DNA translocase activity; responsible for DNA branch migration; required for maintenance of the mitochondrial (mt) genome; localizes to the mitochondrial matrix; monomeric

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_001136.10 Reference assembly

      Range
      1131001..1133070
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NM_001180640.3 → NP_010619.3  TPA: double-stranded DNA-dependent ATPase [Saccharomyces cerevisiae S288C]

      See identical proteins and their annotated locations for NP_010619.3

      Status: REVIEWED

      UniProtKB/Swiss-Prot
      D6VSW4, Q06683
      UniProtKB/TrEMBL
      A0A8H8UNN1, A6ZYT4, B3LFW5, B5VGL5, C7GWM9, C8Z5P4, N1P8N6
      Conserved Domains (1) summary
      COG1061
      Location:19 → 389
      SSL2; Superfamily II DNA or RNA helicase [Transcription, Replication, recombination and repair]
    Nucleotide Protein
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    Q06683.1

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