DPM1 dolichyl-phosphate beta-D-mannosyltransferase [Saccharomyces cerevisiae S288C] - Gene

Summary

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Gene symbol: DPM1
Gene description: dolichyl-phosphate beta-D-mannosyltransferase
Primary source: FungiDB:YPR183W
Locus tag: YPR183W
See related: SGD:S000006387; AllianceGenome:SGD:S000006387; VEuPathDB:YPR183W
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as: SED3
Summary: Enables dolichyl-phosphate beta-D-mannosyltransferase activity. Involved in GPI anchor biosynthetic process and dolichol-linked oligosaccharide biosynthetic process. Located in endoplasmic reticulum and nuclear outer membrane-endoplasmic reticulum membrane network. Human ortholog(s) of this gene implicated in congenital disorder of glycosylation Ie. Orthologous to human DPM1 (dolichyl-phosphate mannosyltransferase subunit 1, catalytic). [provided by Alliance of Genome Resources, Apr 2022]
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Genomic context

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See DPM1 in Genome Data Viewer

Location:: chromosome: XVI

Exon count:: 1

Sequence:: Chromosome: XVI; NC_001148.4 (900755..901558)

Chromosome XVI - NC_001148.4 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001148.4

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Two temperature-sensitive Saccharomyces cerevisiae mutants, sec59-1 and dpm1-6, impaired, respectively, in dolichol kinase (Sec59p) and dolichyl phosphate mannose (DolPMan) synthase (Dpm1p), have an aberrant cell wall structure and composition.
Title: Defect in dolichol-dependent glycosylation increases sensitivity of Saccharomyces cerevisiae towards anti-fungal drugs.
S. cerevisiae DPMS activity is regulated by the cAMP-dependent protein phosphorylation signal, and the phosphorylation target is serine 141
Title: In vitro phosphorylation by cAMP-dependent protein kinase up-regulates recombinant Saccharomyces cerevisiae mannosylphosphodolichol synthase.
A three-dimensional (3D) model of the enzyme was produced with bound substrates, Dol-P, GDP-Man, and divalent cations to delineate the binding sites for these substrates as well as the catalytic site.
Title: Structural studies and mechanism of Saccharomyces cerevisiae dolichyl-phosphate-mannose synthase: insights into the initial step of synthesis of dolichyl-phosphate-linked oligosaccharide chains in membranes of endoplasmic reticulum.
Interaction of Dpm1p with Sac1p persists during exponential cell division but is rapidly abolished when cell growth slows.
Title: Cell growth-dependent coordination of lipid signaling and glycosylation is mediated by interactions between Sac1p and Dpm1p.
Evidence is presented that temperature-sensitive Saccharomyces cerevisiae mutants, impaired in dolichol kinase (Sec59p) or dolichyl phosphate mannose synthase (Dpm1p) activity have an aberrant cell wall composition and ultrastructure.
Title: Dissecting the role of dolichol in cell wall assembly in the yeast mutants impaired in early glycosylation reactions.

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Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables dolichyl-phosphate beta-D-mannosyltransferase activity	IBA Inferred from Biological aspect of Ancestor more info
enables dolichyl-phosphate beta-D-mannosyltransferase activity	IDA Inferred from Direct Assay more info	PubMed
enables dolichyl-phosphate beta-D-mannosyltransferase activity	IEA Inferred from Electronic Annotation more info
enables glycosyltransferase activity	IEA Inferred from Electronic Annotation more info
enables metal ion binding	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in GPI anchor biosynthetic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in GPI anchor biosynthetic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in dolichol-linked oligosaccharide biosynthetic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in dolichol-linked oligosaccharide biosynthetic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in protein O-linked mannosylation	IBA Inferred from Biological aspect of Ancestor more info
involved_in protein glycosylation	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
located_in cell periphery	HDA more info	PubMed
located_in endoplasmic reticulum	HDA more info	PubMed
located_in endoplasmic reticulum	IDA Inferred from Direct Assay more info	PubMed
located_in endoplasmic reticulum	IEA Inferred from Electronic Annotation more info
is_active_in endoplasmic reticulum membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in endoplasmic reticulum membrane	IEA Inferred from Electronic Annotation more info
located_in membrane	IEA Inferred from Electronic Annotation more info
located_in mitochondrial outer membrane	HDA more info	PubMed
located_in mitochondrion	HDA more info	PubMed
located_in nuclear outer membrane-endoplasmic reticulum membrane network	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: dolichyl-phosphate beta-D-mannosyltransferase

NP_015509.1

EC 2.4.1.83

Dolichol phosphate mannose (Dol-P-Man) synthase of ER membrane; catalyzes formation of Dol-P-Man from Dol-P and GDP-Man; required for biosynthesis of glycosyl phosphatidylinositol (GPI) membrane anchor, as well as O-mannosylation and protein N- and O-linked glycosylation; human homolog DPM1 can complement yeast mutant strains

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001148.4 Reference assembly

Range

900755..901558

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001184280.1 → NP_015509.1 TPA: dolichyl-phosphate beta-D-mannosyltransferase [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_015509.1

Status: REVIEWED

UniProtKB/Swiss-Prot

D6W4I3, P14020

UniProtKB/TrEMBL

A0A6A5Q1T9, A6ZX57, B3LKE2, C7GP65, C8ZJL2, G2WPZ4, N1NW70

Conserved Domains (1) summary

cd06442
Location:7 → 234

DPM1_like; DPM1_like represents putative enzymes similar to eukaryotic DPM1