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    THO2 [ Saccharomyces cerevisiae S288C ]

    Gene ID: 855583, updated on 11-Sep-2024

    Summary

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    Official Symbol
    THO2
    Primary source
    SGD:S000005083
    Locus tag
    YNL139C
    See related
    AllianceGenome:SGD:S000005083; FungiDB:YNL139C; VEuPathDB:YNL139C
    Gene type
    protein coding
    RefSeq status
    REVIEWED
    Organism
    Saccharomyces cerevisiae S288C (strain: S288C)
    Lineage
    Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
    Also known as
    LDB5; RLR1; ZRG13
    Summary
    Enables nucleic acid binding activity. Involved in several processes, including nucleic acid metabolic process; positive regulation of transcription elongation from RNA polymerase I promoter; and stress granule assembly. Located in chromosome, telomeric region and nucleus. Part of THO complex part of transcription export complex and nucleoplasmic THO complex. Human ortholog(s) of this gene implicated in X-linked intellectual disability-short stature-overweight syndrome. Orthologous to human THOC2 (THO complex 2). [provided by Alliance of Genome Resources, Apr 2022]
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    Genomic context

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    Location:
    chromosome: XIV
    Exon count:
    1
    Sequence:
    Chromosome: XIV; NC_001146.8 (360924..365717, complement)

    Chromosome XIV - NC_001146.8Genomic Context describing neighboring genes Neighboring gene ammonium permease MEP2 Neighboring gene adenine deaminase Neighboring gene uncharacterized protein Neighboring gene U2 snRNP complex subunit YSF3 Neighboring gene adenylate cyclase-binding protein

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_001146.8

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Molecular evidence that the eukaryotic THO/TREX complex is required for efficient transcription elongation. Rondón AG, et al. J Biol Chem, 2003 Oct 3. PMID 12871933
    2. DEAD-box RNA helicase Sub2 is required for expression of lacZ fusions in Saccharomyces cerevisiae and is a dosage-dependent suppressor of RLR1 (THO2). West RW Jr, et al. Gene, 2002 Apr 17. PMID 12034490
    3. Hpr1 is preferentially required for transcription of either long or G+C-rich DNA sequences in Saccharomyces cerevisiae. Chávez S, et al. Mol Cell Biol, 2001 Oct. PMID 11564888, Free PMC Article
    4. RLR1 (THO2), required for expressing lacZ fusions in yeast, is conserved from yeast to humans and is a suppressor of SIN4. West RW Jr, et al. Gene, 2000 Feb 8. PMID 10675628
    5. Structural and biochemical analyses of the DEAD-box ATPase Sub2 in association with THO or Yra1. Ren Y, et al. Elife, 2017 Jan 6. PMID 28059701, Free PMC Article

    See all (100) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Tho2 is critical for the recruitment of Rrp6 to chromatin in response to perturbed mRNP biogenesis.
      Title: Tho2 is critical for the recruitment of Rrp6 to chromatin in response to perturbed mRNP biogenesis.
    2. Structural insights into the nucleic acid remodeling mechanisms of the yeast THO-Sub2 complex.
      Title: Structural insights into the nucleic acid remodeling mechanisms of the yeast THO-Sub2 complex.
    3. Both THO and Yra1-C stimulated Sub2's intrinsic ATPase activity. The authors propose that THO surveys common landmarks in each nuclear mRNP to localize Sub2 for targeted loading of Yra1.
      Title: Structural and biochemical analyses of the DEAD-box ATPase Sub2 in association with THO or Yra1.
    4. THO2 mutants show defective meiosis and an impairment of premeiotic replication as well as DNA-damage accumulation.
      Title: R-loops cause replication impairment and genome instability during meiosis.
    Submit: New GeneRIF Correction

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Gene Ontology Provided by SGD

    Function Evidence Code Pubs
    enables mRNA binding HDA PubMed 
    enables mRNA binding IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables nucleic acid binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    Process Evidence Code Pubs
    involved_in DNA recombination IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in cellular response to azide IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in mRNA 3'-end processing IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in mRNA export from nucleus IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in mRNA export from nucleus IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in mRNA export from nucleus IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in mRNA processing IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in positive regulation of transcription by RNA polymerase I IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in positive regulation of transcription elongation by RNA polymerase I IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in stress granule assembly IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in transcription elongation by RNA polymerase II IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in transcription-coupled nucleotide-excision repair IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    Component Evidence Code Pubs
    part_of THO complex IEA
    Inferred from Electronic Annotation
    more info
    		  
    part_of THO complex part of transcription export complex IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    part_of THO complex part of transcription export complex IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in chromosome, telomeric region IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    part_of nucleoplasmic THO complex IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    located_in nucleus HDA PubMed 
    located_in nucleus IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in nucleus IEA
    Inferred from Electronic Annotation
    more info
    		  

    General protein information

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    Preferred Names
    Tho2p
    NP_014260.3
    • Subunit of the THO complex; THO is required for efficient transcription elongation and involved in transcriptional elongation-associated recombination; required for LacZ RNA expression from certain plasmids

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_001146.8 Reference assembly

      Range
      360924..365717 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NM_001182977.3 → NP_014260.3  TPA: Tho2p [Saccharomyces cerevisiae S288C]

      See identical proteins and their annotated locations for NP_014260.3

      Status: REVIEWED

      UniProtKB/Swiss-Prot
      D6W142, P53552
      UniProtKB/TrEMBL
      B5VQR0, N1NXE1
      Conserved Domains (3) summary
      pfam11262
      Location:938 → 1192
      Tho2; Transcription factor/nuclear export subunit protein 2
      pfam11732
      Location:658 → 731
      Thoc2; Transcription- and export-related complex subunit
      pfam16134
      Location:30 → 656
      THOC2_N; THO complex subunit 2 N-terminus
    Nucleotide Protein
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    P53552.1

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