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    MED14 mediator complex subunit 14 [ Homo sapiens (human) ]

    Gene ID: 9282, updated on 10-Dec-2024

    Summary

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    Official Symbol
    MED14provided by HGNC
    Official Full Name
    mediator complex subunit 14provided by HGNC
    Primary source
    HGNC:HGNC:2370
    See related
    Ensembl:ENSG00000180182 MIM:300182; AllianceGenome:HGNC:2370
    Gene type
    protein coding
    RefSeq status
    REVIEWED
    Organism
    Homo sapiens
    Lineage
    Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
    Also known as
    CSRP; RGR1; CRSP2; EXLM1; CXorf4; CRSP150; DRIP150; TRAP170
    Summary
    The activation of gene transcription is a multistep process that is triggered by factors that recognize transcriptional enhancer sites in DNA. These factors work with co-activators to direct transcriptional initiation by the RNA polymerase II apparatus. The protein encoded by this gene is a subunit of the CRSP (cofactor required for SP1 activation) complex, which, along with TFIID, is required for efficient activation by SP1. This protein is also a component of other multisubunit complexes e.g. thyroid hormone receptor-(TR-) associated proteins which interact with TR and facilitate TR function on DNA templates in conjunction with initiation factors and cofactors. This protein contains a bipartite nuclear localization signal. This gene is known to escape chromosome X-inactivation. [provided by RefSeq, Jul 2008]
    Expression
    Ubiquitous expression in thyroid (RPKM 8.7), duodenum (RPKM 8.0) and 25 other tissues See more
    Orthologs
    mouse all
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    Genomic context

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    See MED14 in Genome Data Viewer
    Location:
    Xp11.4
    Exon count:
    33
    Annotation release Status Assembly Chr Location
    RS_2024_08 current GRCh38.p14 (GCF_000001405.40) X NC_000023.11 (40648305..40736159, complement)
    RS_2024_08 current T2T-CHM13v2.0 (GCF_009914755.1) X NC_060947.1 (40049523..40137378, complement)
    RS_2024_09 previous assembly GRCh37.p13 (GCF_000001405.25) X NC_000023.10 (40507557..40594794, complement)

    Chromosome X - NC_000023.11Genomic Context describing neighboring genes Neighboring gene chromosome X open reading frame 38 Neighboring gene mitochondrial pyruvate carrier 1 like Neighboring gene Sharpr-MPRA regulatory region 4034 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 29548 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 29549 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 20764 Neighboring gene ReSE screen-validated silencer GRCh37_chrX:40506849-40507029 Neighboring gene ReSE screen-validated silencer GRCh37_chrX:40535358-40535509 Neighboring gene ReSE screen-validated silencer GRCh37_chrX:40537853-40538063 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 20766 Neighboring gene H3K27ac hESC enhancer GRCh37_chrX:40594164-40595085 Neighboring gene H3K27ac hESC enhancer GRCh37_chrX:40595086-40596006 Neighboring gene MED14 opposite strand Neighboring gene ATAC-STARR-seq lymphoblastoid active region 29550 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 29551 Neighboring gene CLDN7 pseudogene 1 Neighboring gene divergent-paired related homeobox pseudogene 6

    Genomic regions, transcripts, and products

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    Go to nucleotide: Graphics FASTA GenBank

    Expression

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    • Project title: Tissue-specific circular RNA induction during human fetal development
    • Description: 35 human fetal samples from 6 tissues (3 - 7 replicates per tissue) collected between 10 and 20 weeks gestational time were sequenced using Illumina TruSeq Stranded Total RNA
    • BioProject: PRJNA270632
    • Publication: PMID 26076956
    • Analysis date: Mon Apr 2 22:54:59 2018

    Bibliography

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    Related articles in PubMed

    1. Detection and isolation of a novel human gene located on Xp11.2-p11.4 that escapes X-inactivation using a two-dimensional DNA mapping method. Yoshikawa H, et al. Genomics, 1998 Apr 15. PMID 9598311
    2. Coactivation of estrogen receptor alpha (ER alpha)/Sp1 by vitamin D receptor interacting protein 150 (DRIP150). Lee J, et al. Arch Biochem Biophys, 2007 May 15. PMID 17306756, Free PMC Article
    3. Reconstitution of active human core Mediator complex reveals a critical role of the MED14 subunit. Cevher MA, et al. Nat Struct Mol Biol, 2014 Dec. PMID 25383669, Free PMC Article
    4. Refinement of the physical location and the genomic characterization of the CRSP2 (EXLM1) gene on Xp11.4. Demirci FY, et al. DNA Seq, 2003 Apr. PMID 12825353
    5. Vitamin D enhances glucocorticoid action in human monocytes: involvement of granulocyte-macrophage colony-stimulating factor and mediator complex subunit 14. Zhang Y, et al. J Biol Chem, 2013 May 17. PMID 23572530, Free PMC Article

    See all (116) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Functional analysis implicates TNRC6A, NAT10, MED14, and WDR5 in RNA-mediated transcriptional activation.
      Title: Human GW182 Paralogs Are the Central Organizers for RNA-Mediated Control of Transcription.
    2. This results from a dramatically enhanced ability of MED14-containing complexes to associate with Pol II.
      Title: Reconstitution of active human core Mediator complex reveals a critical role of the MED14 subunit.
    3. VitD-mediated stimulation of GC anti-inflammatory affects human monocytes in a process involving GM-CSF and MED14
      Title: Vitamin D enhances glucocorticoid action in human monocytes: involvement of granulocyte-macrophage colony-stimulating factor and mediator complex subunit 14.
    4. MED14 and MED1 are used by glucocorticoid receptor in a gene-specific manner, providing a mechanism for promoter selectivity by glucocorticoid receptor
      Title: MED14 and MED1 differentially regulate target-specific gene activation by the glucocorticoid receptor.
    5. CRSP2 gene is expressed in the retina and its exact genomic location is on Xp11.4 between DXS1368 and DXS993
      Title: Refinement of the physical location and the genomic characterization of the CRSP2 (EXLM1) gene on Xp11.4.
    6. Coactivation of ERalpha by DRIP150 in ZR-75 cells is NR box-independent and requires a novel sequence with putative alpha-helical structure.
      Title: DRIP150 coactivation of estrogen receptor alpha in ZR-75 breast cancer cells is independent of LXXLL motifs.
    7. DRIP150 binds to ISGF3 and regulates transcription
      Title: Role of metazoan mediator proteins in interferon-responsive transcription.
    8. Coactivator that enhances estrogen receptor alpha- and specificity protein (SP)-1-mediated transactivation in breast cancer cells.
      Title: Coactivation of estrogen receptor alpha (ER alpha)/Sp1 by vitamin D receptor interacting protein 150 (DRIP150).
    Submit: New GeneRIF Correction

    Phenotypes

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    Review eQTL and phenotype association data in this region using PheGenI

    Variation

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    See variants in ClinVar

    See studies and variants in dbVar

    See Variation Viewer (GRCh37.p13)

    See Variation Viewer (GRCh38)

    HIV-1 interactions

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    Replication interactions

    Interaction Pubs
    Knockdown of mediator complex subunit 14 (MED14) by siRNA inhibits HIV-1 replication in HeLa-derived TZM-bl cells PubMed
    Knockdown of mediator complex subunit 14 (MED14) by siRNA inhibits the early stages of HIV-1 replication in 293T cells infected with VSV-G pseudotyped HIV-1 PubMed

    Protein interactions

    Protein Gene Interaction Pubs
    Tat tat SiRNA knockdown of MED14 shows a significant inhibition in HIV-1 Tat-mediated transcription levels as compared to control PubMed
    tat The interaction of HIV-1 Tat with MED21 hypothetically induces the binding of Tat to MED14 PubMed

    Go to the HIV-1, Human Interaction Database

    Pathways from PubChem

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    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Markers

    Homology

    Clone Names

    • MGC104513

    Gene Ontology Provided by GOA

    Function Evidence Code Pubs
    enables nuclear receptor coactivator activity NAS
    Non-traceable Author Statement
    more info
    		 PubMed 
    enables nuclear vitamin D receptor binding NAS
    Non-traceable Author Statement
    more info
    		 PubMed 
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    enables transcription coactivator activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables transcription coregulator activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables transcription coregulator activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    Process Evidence Code Pubs
    involved_in RNA polymerase II preinitiation complex assembly NAS
    Non-traceable Author Statement
    more info
    		 PubMed 
    involved_in positive regulation of DNA-templated transcription IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    acts_upstream_of_or_within positive regulation of transcription by RNA polymerase II IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in positive regulation of transcription elongation by RNA polymerase II NAS
    Non-traceable Author Statement
    more info
    		 PubMed 
    involved_in positive regulation of transcription initiation by RNA polymerase II IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in positive regulation of transcription initiation by RNA polymerase II NAS
    Non-traceable Author Statement
    more info
    		 PubMed 
    involved_in regulation of transcription by RNA polymerase II IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in somatic stem cell population maintenance IEA
    Inferred from Electronic Annotation
    more info
    		  
    Component Evidence Code Pubs
    part_of core mediator complex IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    part_of core mediator complex IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    part_of mediator complex IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    part_of mediator complex IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in membrane HDA PubMed 
    located_in nucleoplasm TAS
    Traceable Author Statement
    more info
    		  
    located_in nucleus IDA
    Inferred from Direct Assay
    more info
    		 PubMed 

    General protein information

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    Preferred Names
    mediator of RNA polymerase II transcription subunit 14
    Names
    ARC150
    CRSP complex subunit 2
    RGR1 homolog
    activator-recruited cofactor 150 kDa component
    cofactor required for Sp1 transcriptional activation, subunit 2 (150kD)
    hRGR1
    human homolog of yeast RGR1
    thyroid hormone receptor-associated protein complex 170 kDa component
    thyroid hormone receptor-associated protein complex component TRAP170
    transcriptional co-activator CRSP150
    vitamin D receptor-interacting protein complex component DRIP150
    vitamin D3 receptor-interacting protein complex 150 kDa component

    NCBI Reference Sequences (RefSeq)

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    RefSeqs maintained independently of Annotated Genomes

    These reference sequences exist independently of genome builds. Explain

    These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

    Genomic

    1. NG_016325.2 RefSeqGene

      Range
      5617..92854
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NM_004229.4 → NP_004220.2  mediator of RNA polymerase II transcription subunit 14

      See identical proteins and their annotated locations for NP_004220.2

      Status: REVIEWED

      Source sequence(s)
      AC093029, AF304448, BC098377
      Consensus CDS
      CCDS14254.1
      UniProtKB/Swiss-Prot
      O60244, Q4KMR7, Q9UNB3
      UniProtKB/TrEMBL
      A8KAK5
      Related
      ENSP00000323720.1, ENST00000324817.6
      Conserved Domains (2) summary
      pfam05109
      Location:1015 → 1189
      Herpes_BLLF1; Herpes virus major outer envelope glycoprotein (BLLF1)
      pfam08638
      Location:51 → 238
      Med14; Mediator complex subunit MED14

    RefSeqs of Annotated Genomes: GCF_000001405.40-RS_2024_08

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference GRCh38.p14 Primary Assembly

    Genomic

    1. NC_000023.11 Reference GRCh38.p14 Primary Assembly

      Range
      40648305..40736159 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. XM_047442636.1 → XP_047298592.1  mediator of RNA polymerase II transcription subunit 14 isoform X1

      UniProtKB/TrEMBL
      A8KAK5

    2. XM_047442638.1 → XP_047298594.1  mediator of RNA polymerase II transcription subunit 14 isoform X3

      UniProtKB/TrEMBL
      A8KAK5

    3. XM_047442643.1 → XP_047298599.1  mediator of RNA polymerase II transcription subunit 14 isoform X8

      UniProtKB/TrEMBL
      A8KAK5

    4. XM_047442641.1 → XP_047298597.1  mediator of RNA polymerase II transcription subunit 14 isoform X6

      UniProtKB/TrEMBL
      A8KAK5

    5. XM_047442640.1 → XP_047298596.1  mediator of RNA polymerase II transcription subunit 14 isoform X5

      UniProtKB/TrEMBL
      A8KAK5

    6. XM_047442637.1 → XP_047298593.1  mediator of RNA polymerase II transcription subunit 14 isoform X2

      UniProtKB/TrEMBL
      A8KAK5

    7. XM_047442642.1 → XP_047298598.1  mediator of RNA polymerase II transcription subunit 14 isoform X7

      UniProtKB/TrEMBL
      A8KAK5

    8. XM_047442639.1 → XP_047298595.1  mediator of RNA polymerase II transcription subunit 14 isoform X4

      UniProtKB/TrEMBL
      A8KAK5

    Alternate T2T-CHM13v2.0

    Genomic

    1. NC_060947.1 Alternate T2T-CHM13v2.0

      Range
      40049523..40137378 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. XM_054328126.1 → XP_054184101.1  mediator of RNA polymerase II transcription subunit 14 isoform X1

      UniProtKB/TrEMBL
      A8KAK5

    2. XM_054328128.1 → XP_054184103.1  mediator of RNA polymerase II transcription subunit 14 isoform X3

      UniProtKB/TrEMBL
      A8KAK5

    3. XM_054328133.1 → XP_054184108.1  mediator of RNA polymerase II transcription subunit 14 isoform X8

      UniProtKB/TrEMBL
      A8KAK5

    4. XM_054328131.1 → XP_054184106.1  mediator of RNA polymerase II transcription subunit 14 isoform X6

      UniProtKB/TrEMBL
      A8KAK5

    5. XM_054328130.1 → XP_054184105.1  mediator of RNA polymerase II transcription subunit 14 isoform X5

      UniProtKB/TrEMBL
      A8KAK5

    6. XM_054328127.1 → XP_054184102.1  mediator of RNA polymerase II transcription subunit 14 isoform X2

      UniProtKB/TrEMBL
      A8KAK5

    7. XM_054328132.1 → XP_054184107.1  mediator of RNA polymerase II transcription subunit 14 isoform X7

      UniProtKB/TrEMBL
      A8KAK5

    8. XM_054328129.1 → XP_054184104.1  mediator of RNA polymerase II transcription subunit 14 isoform X4

      UniProtKB/TrEMBL
      A8KAK5
    Nucleotide Protein
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    O60244.2 GenPept UniProtKB/Swiss-Prot:O60244

    Gene LinkOut

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