cad carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase [Danio rerio (zebrafish)] - Gene

Summary

Official Symbol: cadprovided by ZNC
Official Full Name: carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotaseprovided by ZNC
Primary source: ZFIN:ZDB-GENE-021030-4
See related: Ensembl:ENSDARG00000041895 AllianceGenome:ZFIN:ZDB-GENE-021030-4
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Danio rerio
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes; Danionidae; Danioninae; Danio
Also known as: cb456; wu:fc30c12; wu:fc33d01; wu:fc67g02; si:dkey-221h15.3
Summary: Predicted to enable aspartate carbamoyltransferase activity and dihydroorotase activity. Predicted to contribute to carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity. Acts upstream of or within several processes, including 'de novo' pyrimidine nucleobase biosynthetic process; cranial nerve morphogenesis; and regulation of signal transduction. Predicted to be active in cytosol. Is expressed in several structures, including digestive system; immature eye; nervous system; neural tube; and pectoral fin. Human ortholog(s) of this gene implicated in developmental and epileptic encephalopathy 50. Orthologous to human CAD (carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase). [provided by Alliance of Genome Resources, Nov 2024]
Orthologs: human mouse all
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Genomic context

Location:: chromosome: 20

Exon count:: 45

Annotation release	Status	Assembly	Chr	Location
RS_2024_08	current	GRCz11 (GCF_000002035.6)	20	NC_007131.7 (38788319..38827736, complement)
105	previous assembly	GRCz10 (GCF_000002035.5)	20	NC_007131.6 (38885440..38925163, complement)

Chromosome 20 - NC_007131.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

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Expression

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See details

Project title: Sequencing the Zebrafish transcriptome from a range of tissues and developmental stages
Description: Sequencing the Zebrafish transcriptome from a range of tissues and developmental stages
BioProject: PRJEB1986
Analysis date: Fri Dec 8 19:48:10 2017

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

These findings suggest important posttranslational modifications requiring Cad as an unappreciated mechanism that regulates Notch/Vegf signaling during angiogenesis.
Title: carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase (cad) regulates Notch signaling and vascular development in zebrafish.
This study demonistrated that a novel role for carbamoyl phosphate synthetase 2 in cranial sensory circuit formation.
Title: Novel role for carbamoyl phosphate synthetase 2 in cranial sensory circuit formation.
an essential role for CAD in facilitating proliferation and differentiation events in a tissue-specific manner
Title: Analysis of the Zebrafish perplexed mutation reveals tissue-specific roles for de novo pyrimidine synthesis during development.

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General gene information

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Markers

AI722689 (e-PCR)
- UniSTS:205993

fc67g02.x1 (e-PCR)
- UniSTS:194229

Gene Ontology Provided by ZFIN

Function	Evidence Code	Pubs
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables amino acid binding	IEA Inferred from Electronic Annotation more info
enables aspartate carbamoyltransferase activity	IEA Inferred from Electronic Annotation more info
enables carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity	IEA Inferred from Electronic Annotation more info
enables carboxyl- or carbamoyltransferase activity	IEA Inferred from Electronic Annotation more info
enables catalytic activity	IEA Inferred from Electronic Annotation more info
enables dihydroorotase activity	IEA Inferred from Electronic Annotation more info
enables hydrolase activity	IEA Inferred from Electronic Annotation more info
enables hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds	IEA Inferred from Electronic Annotation more info
enables hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in cyclic amides	IEA Inferred from Electronic Annotation more info
enables ligase activity	IEA Inferred from Electronic Annotation more info
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables nucleotide binding	IEA Inferred from Electronic Annotation more info
enables transferase activity	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in 'de novo' UMP biosynthetic process	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within 'de novo' pyrimidine nucleobase biosynthetic process	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within 'de novo' pyrimidine nucleobase biosynthetic process	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within amino acid metabolic process	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within facial nerve morphogenesis	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within glossopharyngeal nerve morphogenesis	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within glutamine metabolic process	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within lymphangiogenesis	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within neuron projection morphogenesis	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within pyrimidine nucleotide biosynthetic process	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within regulation of Notch signaling pathway	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within regulation of vascular endothelial growth factor receptor signaling pathway	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within regulation of vascular endothelial growth factor signaling pathway	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within retina development in camera-type eye	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within trigeminal nerve morphogenesis	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within vagus nerve morphogenesis	IMP Inferred from Mutant Phenotype more info	PubMed

General protein information

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Preferred Names: multifunctional protein CAD

NP_001295754.1

EC 2.1.3.2

XP_005160868.1

EC 2.1.3.2

XP_009293160.1

EC 2.1.3.2

XP_009293161.1

EC 2.1.3.2

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001308825.1 → NP_001295754.1 multifunctional protein CAD

Status: VALIDATED

Source sequence(s)

BX511178

UniProtKB/TrEMBL

A0A8M1P7L3, U3JAV8

Related

ENSDARP00000127377.1, ENSDART00000153310.3

Conserved Domains (12) summary

cd01744
Location:182 → 358

GATase1_CPSase; Small chain of the glutamine-dependent form of carbamoyl phosphate synthase, CPSase II

PRK00856
Location:1926 → 2230

pyrB; aspartate carbamoyltransferase catalytic subunit; Provisional

PRK12564
Location:4 → 359

PRK12564; carbamoyl phosphate synthase small subunit; Reviewed

cd01316
Location:1466 → 1812

CAD_DHOase; The eukaryotic CAD protein is a trifunctional enzyme of carbamoylphosphate synthetase-aspartate transcarbamoylase-dihydroorotase, which catalyzes the first three steps of de novo pyrimidine nucleotide biosynthesis. Dihydroorotase (DHOase) catalyzes the ...

cd01423
Location:1318 → 1444

MGS_CPS_I_III; Methylglyoxal synthase-like domain found in pyr1 and URA1-like carbamoyl phosphate synthetases (CPS), including ammonia-dependent CPS Type I, and glutamine-dependent CPS Type III. These are multidomain proteins, in which MGS is the C-terminal domain.

TIGR01369
Location:395 → 1446

CPSaseII_lrg; carbamoyl-phosphate synthase, large subunit

pfam00185
Location:2078 → 2227

OTCace; Aspartate/ornithine carbamoyltransferase, Asp/Orn binding domain

pfam00988
Location:6 → 142

CPSase_sm_chain; Carbamoyl-phosphate synthase small chain, CPSase domain

pfam02729
Location:1931 → 2071

OTCace_N; Aspartate/ornithine carbamoyltransferase, carbamoyl-P binding domain

pfam02786
Location:516 → 718

CPSase_L_D2; Carbamoyl-phosphate synthase L chain, ATP binding domain

pfam02787
Location:803 → 926

CPSase_L_D3; Carbamoyl-phosphate synthetase large chain, oligomerization domain

cl17255
Location:1074 → 1250

ATP-grasp_4; ATP-grasp domain

RefSeqs of Annotated Genomes: GCF_000002035.6-RS_2024_08

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCz11 Primary Assembly

Genomic

NC_007131.7 Reference GRCz11 Primary Assembly

Range

38788319..38827736 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_009294886.4 → XP_009293161.1 multifunctional protein CAD isoform X2

UniProtKB/TrEMBL

A0A8M3B4B1

Conserved Domains (12) summary

cd01744
Location:182 → 358

GATase1_CPSase; Small chain of the glutamine-dependent form of carbamoyl phosphate synthase, CPSase II

PRK00856
Location:1939 → 2243

pyrB; aspartate carbamoyltransferase catalytic subunit; Provisional

PRK12564
Location:4 → 359

PRK12564; carbamoyl phosphate synthase small subunit; Reviewed

cd01316
Location:1466 → 1812

CAD_DHOase; The eukaryotic CAD protein is a trifunctional enzyme of carbamoylphosphate synthetase-aspartate transcarbamoylase-dihydroorotase, which catalyzes the first three steps of de novo pyrimidine nucleotide biosynthesis. Dihydroorotase (DHOase) catalyzes the ...

cd01423
Location:1318 → 1444

MGS_CPS_I_III; Methylglyoxal synthase-like domain found in pyr1 and URA1-like carbamoyl phosphate synthetases (CPS), including ammonia-dependent CPS Type I, and glutamine-dependent CPS Type III. These are multidomain proteins, in which MGS is the C-terminal domain.

TIGR01369
Location:395 → 1446

CPSaseII_lrg; carbamoyl-phosphate synthase, large subunit

pfam00185
Location:2091 → 2240

OTCace; Aspartate/ornithine carbamoyltransferase, Asp/Orn binding domain

pfam00988
Location:6 → 142

CPSase_sm_chain; Carbamoyl-phosphate synthase small chain, CPSase domain

pfam02729
Location:1944 → 2084

OTCace_N; Aspartate/ornithine carbamoyltransferase, carbamoyl-P binding domain

pfam02786
Location:516 → 718

CPSase_L_D2; Carbamoyl-phosphate synthase L chain, ATP binding domain

pfam02787
Location:803 → 926

CPSase_L_D3; Carbamoyl-phosphate synthetase large chain, oligomerization domain

cl17255
Location:1074 → 1250

ATP-grasp_4; ATP-grasp domain
XM_005160811.5 → XP_005160868.1 multifunctional protein CAD isoform X3

UniProtKB/TrEMBL

A0A8M2B804

Conserved Domains (12) summary

cd01744
Location:182 → 358

GATase1_CPSase; Small chain of the glutamine-dependent form of carbamoyl phosphate synthase, CPSase II

PRK00856
Location:1939 → 2243

pyrB; aspartate carbamoyltransferase catalytic subunit; Provisional

PRK12564
Location:4 → 359

PRK12564; carbamoyl phosphate synthase small subunit; Reviewed

cd01316
Location:1466 → 1812

CAD_DHOase; The eukaryotic CAD protein is a trifunctional enzyme of carbamoylphosphate synthetase-aspartate transcarbamoylase-dihydroorotase, which catalyzes the first three steps of de novo pyrimidine nucleotide biosynthesis. Dihydroorotase (DHOase) catalyzes the ...

cd01423
Location:1318 → 1444

MGS_CPS_I_III; Methylglyoxal synthase-like domain found in pyr1 and URA1-like carbamoyl phosphate synthetases (CPS), including ammonia-dependent CPS Type I, and glutamine-dependent CPS Type III. These are multidomain proteins, in which MGS is the C-terminal domain.

TIGR01369
Location:395 → 1446

CPSaseII_lrg; carbamoyl-phosphate synthase, large subunit

pfam00185
Location:2091 → 2240

OTCace; Aspartate/ornithine carbamoyltransferase, Asp/Orn binding domain

pfam00988
Location:6 → 142

CPSase_sm_chain; Carbamoyl-phosphate synthase small chain, CPSase domain

pfam02729
Location:1944 → 2084

OTCace_N; Aspartate/ornithine carbamoyltransferase, carbamoyl-P binding domain

pfam02786
Location:516 → 718

CPSase_L_D2; Carbamoyl-phosphate synthase L chain, ATP binding domain

pfam02787
Location:803 → 926

CPSase_L_D3; Carbamoyl-phosphate synthetase large chain, oligomerization domain

cl17255
Location:1074 → 1250

ATP-grasp_4; ATP-grasp domain
XM_009294885.4 → XP_009293160.1 multifunctional protein CAD isoform X1

UniProtKB/TrEMBL

A0A8M3AL15

Conserved Domains (12) summary

cd01744
Location:182 → 358

GATase1_CPSase; Small chain of the glutamine-dependent form of carbamoyl phosphate synthase, CPSase II

PRK00856
Location:1952 → 2256

pyrB; aspartate carbamoyltransferase catalytic subunit; Provisional

PRK12564
Location:4 → 359

PRK12564; carbamoyl phosphate synthase small subunit; Reviewed

cd01316
Location:1466 → 1812

CAD_DHOase; The eukaryotic CAD protein is a trifunctional enzyme of carbamoylphosphate synthetase-aspartate transcarbamoylase-dihydroorotase, which catalyzes the first three steps of de novo pyrimidine nucleotide biosynthesis. Dihydroorotase (DHOase) catalyzes the ...

cd01423
Location:1318 → 1444

MGS_CPS_I_III; Methylglyoxal synthase-like domain found in pyr1 and URA1-like carbamoyl phosphate synthetases (CPS), including ammonia-dependent CPS Type I, and glutamine-dependent CPS Type III. These are multidomain proteins, in which MGS is the C-terminal domain.

TIGR01369
Location:395 → 1446

CPSaseII_lrg; carbamoyl-phosphate synthase, large subunit

pfam00185
Location:2104 → 2253

OTCace; Aspartate/ornithine carbamoyltransferase, Asp/Orn binding domain

pfam00988
Location:6 → 142

CPSase_sm_chain; Carbamoyl-phosphate synthase small chain, CPSase domain

pfam02729
Location:1957 → 2097

OTCace_N; Aspartate/ornithine carbamoyltransferase, carbamoyl-P binding domain

pfam02786
Location:516 → 718

CPSase_L_D2; Carbamoyl-phosphate synthase L chain, ATP binding domain

pfam02787
Location:803 → 926

CPSase_L_D3; Carbamoyl-phosphate synthetase large chain, oligomerization domain

cl17255
Location:1074 → 1250

ATP-grasp_4; ATP-grasp domain

Suppressed Reference Sequence(s)

The following Reference Sequences have been suppressed. Explain

NM_001009884.1: Suppressed sequence

Description

NM_001009884.1: This RefSeq was removed because currently there is insufficient support for the transcript and the protein.