NET1 [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: NET1
Primary source: SGD:S000003612
Locus tag: YJL076W
See related: AllianceGenome:SGD:S000003612; FungiDB:YJL076W; VEuPathDB:YJL076W
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as: CFI1; ESC5; SRM8
Summary: Enables protein phosphatase inhibitor activity and rDNA binding activity. Involved in negative regulation of exit from mitosis; positive regulation of protein localization to nucleolus; and rDNA heterochromatin assembly. Located in nucleolus. Part of RENT complex. [provided by Alliance of Genome Resources, Apr 2022]
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Genomic context

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Location:: chromosome: X

Exon count:: 1

Sequence:: Chromosome: X; NC_001142.9 (295245..298814)

Chromosome X - NC_001142.9 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001142.9

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

In Saccharomyces cerevisiae Hog1 delays mitotic exit when cells are stressed during metaphase. Hog1 phosphorylates the nucleolar protein Net1, altering its affinity for the phosphatase Cdc14, whose activity is essential for mitotic exit and completion of the cell cycle. Saccharomyces cerevisiae strain with mutant Net1 that cannot be phosphorylated by Hog1 displays reduced viability upon osmostress.
Title: Hog1 activation delays mitotic exit via phosphorylation of Net1.
Results describe the C-terminal region of Net1 as an activator of RNA polymerase I transcription in baker's yeast. In the absence of this activator RNA polymerase I transcription is downregulated and cell proliferation is strongly impaired. Strikingly, this activator might be conserved in human cells, which points to a general mechanism.
Title: The C-terminal region of Net1 is an activator of RNA polymerase I transcription with conserved features from yeast to human.
genes SRM5/CDC28, SRM12/HFI1/ADA1, and SRM8/NET1 are involved in the formation of a cell response to DNA damage, and in checkpoint regulation in particular.
Title: [Participation of SRM5/CDC28, SRM8/NET1, and SRM12/HFI1 genes in checkpoint control in yeast Saccharomyces cerevisiae].
Results show that PP2A(Cdc55) phosphatase keeps Net1 underphosphorylated in metaphase, and that separase interacts with and downregulates PP2A(Cdc55), thereby facilitating Cdk-dependent Net1 phosphorylation.
Title: Downregulation of PP2A(Cdc55) phosphatase by separase initiates mitotic exit in budding yeast.
results show that the FEAR network acts in part by stimulating phosphorylation of Net1 by Clb-Cdk, which directly weakens the interaction of Cdc14 with Net1, allowing for Cdc14 activation and mitotic exit
Title: Phosphorylation by cyclin B-Cdk underlies release of mitotic exit activator Cdc14 from the nucleolus.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables phosphatase activator activity	IBA Inferred from Biological aspect of Ancestor more info
enables protein phosphatase inhibitor activity	IDA Inferred from Direct Assay more info	PubMed
enables rDNA binding	IBA Inferred from Biological aspect of Ancestor more info
enables rDNA binding	IDA Inferred from Direct Assay more info	PubMed

Process	Evidence Code	Pubs
involved_in cell division	IEA Inferred from Electronic Annotation more info
involved_in negative regulation of exit from mitosis	IGI Inferred from Genetic Interaction more info	PubMed
involved_in negative regulation of exit from mitosis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of septation initiation signaling	IBA Inferred from Biological aspect of Ancestor more info
involved_in nucleolus organization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of protein localization to nucleolus	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within protein localization to nucleolus	IDA Inferred from Direct Assay more info	PubMed
involved_in rDNA heterochromatin formation	IBA Inferred from Biological aspect of Ancestor more info
involved_in rDNA heterochromatin formation	IEA Inferred from Electronic Annotation more info
involved_in rDNA heterochromatin formation	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
part_of RENT complex	IDA Inferred from Direct Assay more info	PubMed
is_active_in nucleolus	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleolus	IDA Inferred from Direct Assay more info	PubMed
located_in nucleolus	IEA Inferred from Electronic Annotation more info
located_in nucleus	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: Net1p

NP_012459.1

Core subunit of the RENT complex; involved in nucleolar silencing and telophase exit; stimulates transcription by RNA polymerase I and regulates nucleolar structure; NET1 has a paralog, TOF2, that arose from the whole genome duplication

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001142.9 Reference assembly

Range

295245..298814

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001181509.1 → NP_012459.1 TPA: Net1p [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_012459.1

Status: REVIEWED

UniProtKB/Swiss-Prot

D6VWA8, P47035

UniProtKB/TrEMBL

N1P8G4

Conserved Domains (2) summary

pfam09528
Location:472 → 963

Ehrlichia_rpt; Ehrlichia tandem repeat (Ehrlichia_rpt)

pfam10407
Location:71 → 142

Cytokin_check_N; Cdc14 phosphatase binding protein N-terminus