SSZ1 Ssz1p [Saccharomyces cerevisiae S288C] - Gene

Summary

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Gene symbol: SSZ1
Gene description: Ssz1p
Primary source: FungiDB:YHR064C
Locus tag: YHR064C
See related: SGD:S000001106; AllianceGenome:SGD:S000001106; VEuPathDB:YHR064C
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as: PDR13
Summary: Enables unfolded protein binding activity. Involved in several processes, including 'de novo' cotranslational protein folding; regulation of translational fidelity; and translation. Located in cytoplasm. Part of polysome. Orthologous to human HSPA14 (heat shock protein family A (Hsp70) member 14). [provided by Alliance of Genome Resources, Apr 2022]
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Genomic context

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See SSZ1 in Genome Data Viewer

Location:: chromosome: VIII

Exon count:: 1

Sequence:: Chromosome: VIII; NC_001140.6 (225525..227141, complement)

Chromosome VIII - NC_001140.6 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001140.6

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Structural remodeling of ribosome associated Hsp40-Hsp70 chaperones during co-translational folding.
Title: Structural remodeling of ribosome associated Hsp40-Hsp70 chaperones during co-translational folding.
Pathway of Hsp70 interactions at the ribosome.
Title: Pathway of Hsp70 interactions at the ribosome.
Ssz1 is catalytically inert and cannot adopt the closed conformation, but the substrate binding domain beta is completed by Zuo1.
Title: Structural insights into a unique Hsp70-Hsp40 interaction in the eukaryotic ribosome-associated complex.
The yeast ribosome-associated complex is composed of HSP40 (Zuotin) and HSP70 (Ssz1). RAC stabilizes the 80S ribosome in nonrotated, classical conformation.
Title: Structural basis for interaction of a cotranslational chaperone with the eukaryotic ribosome.
study presents structural analyses of the ribosome-associated complex (RAC) consisting (Hsp70) Ssz1 and the Hsp40 Zuo1; crystal structure of the Ssz1 ATPase domain bound to ATP-Mg(2) explains its catalytic inactivity and suggests Ssz1 may act before the RAC-associated chaperone Ssb
Title: Structural characterization of a eukaryotic chaperone--the ribosome-associated complex.
Ssz1 activates Pdr1 causing premature growth arrest of cells during the diauxic shift, through quorum sensing, as they adapt to the changing environmental conditions.
Title: Role for the molecular chaperones Zuo1 and Ssz1 in quorum sensing via activation of the transcription factor Pdr1.
Ssz1 restores endoplasmic reticulum-associated protein degradation in cells expressing defective cdc48-ufd1-npl4 complex by upregulating cdc48.
Title: Ssz1 restores endoplasmic reticulum-associated protein degradation in cells expressing defective cdc48-ufd1-npl4 complex by upregulating cdc48.
structural analysis of teh ribosome-associated complex (RAC) reveals an unusual Hsp70/Hsp40 interaction between zuotin and ssz
Title: Structural analysis of the ribosome-associated complex (RAC) reveals an unusual Hsp70/Hsp40 interaction.
Zuo1 and Ssz1 together with the Hsp70 homolog Ssb1/2 form a functional triad involved in translation and early polypeptide folding processes.
Title: Functional characterization of the atypical Hsp70 subunit of yeast ribosome-associated complex.
Ssz1 binds ATP, but none of the 11 different amino acid substitutions in the ATP-binding cleft affected Ssz1 function in vivo.
Title: The Hsp70 Ssz1 modulates the function of the ribosome-associated J-protein Zuo1.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables ATP hydrolysis activity	IBA Inferred from Biological aspect of Ancestor more info
enables ATP-dependent protein folding chaperone	IEA Inferred from Electronic Annotation more info
enables heat shock protein binding	IBA Inferred from Biological aspect of Ancestor more info
enables protein folding chaperone	IBA Inferred from Biological aspect of Ancestor more info
enables unfolded protein binding	IMP Inferred from Mutant Phenotype more info	PubMed

Process	Evidence Code	Pubs
involved_in 'de novo' cotranslational protein folding	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in chaperone cofactor-dependent protein refolding	IBA Inferred from Biological aspect of Ancestor more info
involved_in cytoplasmic translation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in protein refolding	IBA Inferred from Biological aspect of Ancestor more info
involved_in rRNA processing	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of translational fidelity	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in translational frameshifting	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasm	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: Ssz1p

NP_011931.2

Hsp70 protein that interacts with Zuo1p (a DnaJ homolog); interacts with Zuo1p to form a ribosome-associated complex that binds the ribosome via the Zuo1p subunit; also involved in pleiotropic drug resistance via sequential activation of PDR1 and PDR5; binds ATP

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001140.6 Reference assembly

Range

225525..227141 complement

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001179194.1 → NP_011931.2 TPA: Ssz1p [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_011931.2

Status: REVIEWED

UniProtKB/Swiss-Prot

D3DL13, P38788, Q6B1F3, Q6TQT7, Q6TQT8

UniProtKB/TrEMBL

A6ZSX0, G2WFD6

Conserved Domains (1) summary

cd10232
Location:4 → 384

ScSsz1p_like_NBD; Nucleotide-binding domain of Saccharmomyces cerevisiae Ssz1pp and similar proteins