CANX calnexin [Homo sapiens (human)] - Gene

Summary

Official Symbol: CANXprovided by HGNC
Official Full Name: calnexinprovided by HGNC
Primary source: HGNC:HGNC:1473
See related: Ensembl:ENSG00000127022 MIM:114217; AllianceGenome:HGNC:1473
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Homo sapiens
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
Also known as: CNX; P90; IP90
Summary: This gene encodes a member of the calnexin family of molecular chaperones. The encoded protein is a calcium-binding, endoplasmic reticulum (ER)-associated protein that interacts transiently with newly synthesized N-linked glycoproteins, facilitating protein folding and assembly. It may also play a central role in the quality control of protein folding by retaining incorrectly folded protein subunits within the ER for degradation. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Jun 2018]
Expression: Ubiquitous expression in thyroid (RPKM 267.4), placenta (RPKM 108.5) and 25 other tissues See more
Orthologs: mouse all
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Genomic context

Location:: 5q35.3

Exon count:: 21

Annotation release	Status	Assembly	Chr	Location
RS_2024_08	current	GRCh38.p14 (GCF_000001405.40)	5	NC_000005.10 (179678656..179731641)
RS_2024_08	current	T2T-CHM13v2.0 (GCF_009914755.1)	5	NC_060929.1 (180233830..180286826)
RS_2024_09	previous assembly	GRCh37.p13 (GCF_000001405.25)	5	NC_000005.9 (179125428..179158642)

Chromosome 5 - NC_000005.10 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: HPA RNA-seq normal tissues
Description: RNA-seq was performed of tissue samples from 95 human individuals representing 27 different tissues in order to determine tissue-specificity of all protein-coding genes
BioProject: PRJEB4337
Publication: PMID 24309898
Analysis date: Wed Apr 4 07:08:55 2018

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

PON2 ameliorates Ang II-induced cardiomyocyte injury by targeting the CANX/NOX4 signaling pathway.
Title: PON2 ameliorates Ang II-induced cardiomyocyte injury by targeting the CANX/NOX4 signaling pathway.
KAT7-mediated CANX (calnexin) crotonylation regulates leucine-stimulated MTORC1 activity.
Title: KAT7-mediated CANX (calnexin) crotonylation regulates leucine-stimulated MTORC1 activity.
A Chaperone-Like Role for EBI3 in Collaboration With Calnexin Under Inflammatory Conditions.
Title: A Chaperone-Like Role for EBI3 in Collaboration With Calnexin Under Inflammatory Conditions.
Circulating small extracellular vesicles increase after an acute bout of moderate-intensity exercise in pregnant compared to non-pregnant women.
Title: Circulating small extracellular vesicles increase after an acute bout of moderate-intensity exercise in pregnant compared to non-pregnant women.
The Role of Cardiolipin as a Scaffold Mitochondrial Phospholipid in Autophagosome Formation: In Vitro Evidence.
Title: The Role of Cardiolipin as a Scaffold Mitochondrial Phospholipid in Autophagosome Formation: In Vitro Evidence.
Ca(2+) Regulates ERp57-Calnexin Complex Formation.
Title: Ca(2+) Regulates ERp57-Calnexin Complex Formation.
Calnexin mediates the maturation of GPI-anchors through ER retention.
Title: Calnexin mediates the maturation of GPI-anchors through ER retention.
ER-resident oxidoreductases are glycosylated and trafficked to the cell surface to promote matrix degradation by tumour cells.
Title: ER-resident oxidoreductases are glycosylated and trafficked to the cell surface to promote matrix degradation by tumour cells.
Calnexin accelerates the folding of the mucopolysaccharidosis type II mutant IDS in the endoplasmic reticulum.
Title: Calnexin promotes the folding of mutant iduronate 2-sulfatase related to mucopolysaccharidosis type II.
This work uncovers a mechanism by which T-cell antitumor responses are regulated by calnexin in tumor cells and suggests that calnexin might serve as a potential target for the improvement of antitumor immunotherapy.
Title: Calnexin Impairs the Antitumor Immunity of CD4(+) and CD8(+) T Cells.

Submit: New GeneRIF Correction See all GeneRIFs (65)

Phenotypes

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Find tests for this gene in the NIH Genetic Testing Registry (GTR)

Review eQTL and phenotype association data in this region using PheGenI

EBI GWAS Catalog

Description
Insight into genetic determinants of resting heart rate. EBI GWAS Catalog EBI GWAS CatalogPubMed

Variation

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See variants in ClinVar

See studies and variants in dbVar

See Variation Viewer (GRCh37.p13)

See Variation Viewer (GRCh38)

HIV-1 interactions

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Protein interactions

Protein	Gene	Interaction	Pubs
Envelope surface glycoprotein gp120	env	Tandem affinity purification and mass spectrometry analysis identify calnexin (CANX), HIV-1 Gag, Gag/Pol, gp120, and Nef incorporated into staufen1 RNP complexes isolated from HIV-1-expressing cells	PubMed
	env	The signal-sequence-retained form of HIV-1 gp120 is associated with calnexin in the endoplasmic reticulum (ER), while the signal-sequence-cleaved form is disassociated from calnexin and undergoes folding to associate with the CD4 cellular receptor	PubMed
Envelope surface glycoprotein gp160, precursor	env	HIV-1 gp160 interacts with CANX; predicted interaction to be within the endoplasmic reticulum and function as chaperone for endoplasmic reticulum-associated degradation	PubMed
	env	HIV-1 Nef enhances interaction of calnexin with HIV-1 gp160, but inhibits physical interaction between calnexin and ABCA1	PubMed
	env	HIV-1 gp160 domains V1/V2, C2, and the C-terminus of V3 are required for its binding to calnexin	PubMed
	env	HIV-1 gp160 is identified to have a physical interaction with calnexin (CANX) in human HEK293 and/or Jurkat cell lines by using affinity tagging and purification mass spectrometry analyses	PubMed
	env	The molecular chaperone calnexin binds to newly synthesized HIV-1 gp160; nonglycosylated gp160 is not associated with calnexin, suggesting a requirement for N-linked oligosaccharides on the newly synthesized protein for binding to occur	PubMed
Gag-Pol	gag-pol	Tandem affinity purification and mass spectrometry analysis identify calnexin (CANX), HIV-1 Gag, Gag/Pol, gp120, and Nef incorporated into staufen1 RNP complexes isolated from HIV-1-expressing cells	PubMed
Nef	nef	Knockdown of calnexin by siRNA mimics the effects of HIV-1 Nef on ABCA1 downregulation in cells	PubMed
	nef	HIV-1 Nef physically interacts with calnexin and co-localizes with calnexin to ER membranes in cells	PubMed
	nef	Tandem affinity purification and mass spectrometry analysis identify calnexin (CANX), HIV-1 Gag, Gag/Pol, gp120, and Nef incorporated into staufen1 RNP complexes isolated from HIV-1-expressing cells	PubMed
Pr55(Gag)	gag	Tandem affinity purification and mass spectrometry analysis identify calnexin (CANX), HIV-1 Gag, Gag/Pol, gp120, and Nef incorporated into staufen1 RNP complexes isolated from HIV-1-expressing cells	PubMed
Tat	tat	Expression of HIV-1 Tat upregulates the abundance of calnexin (CANX) in the nucleoli of Jurkat T-cells	PubMed

Go to the HIV-1, Human Interaction Database

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

RH44837 (e-PCR)
- UniSTS:6187

RH17758 (e-PCR)
- UniSTS:39757

RH16379 (e-PCR)
- UniSTS:26512

108 (e-PCR), detects polymorphism
- UniSTS:17566

SHGC-52564 (e-PCR)
- UniSTS:67026

STS-N25670 (e-PCR)
- UniSTS:60887

SHGC-12400 (e-PCR)
- UniSTS:9459

D5S2779 (e-PCR)
- UniSTS:473847

Gene Ontology Provided by GOA

Function	Evidence Code	Pubs
enables RNA binding	HDA more info	PubMed
enables calcium ion binding	IBA Inferred from Biological aspect of Ancestor more info
enables carbohydrate binding	IEA Inferred from Electronic Annotation more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables unfolded protein binding	TAS Traceable Author Statement more info

Process	Evidence Code	Pubs
involved_in ERAD pathway	IBA Inferred from Biological aspect of Ancestor more info
involved_in clathrin-dependent endocytosis	ISS Inferred from Sequence or Structural Similarity more info
involved_in protein folding	IBA Inferred from Biological aspect of Ancestor more info
involved_in protein folding in endoplasmic reticulum	TAS Traceable Author Statement more info	PubMed
involved_in protein secretion	TAS Traceable Author Statement more info	PubMed
involved_in synaptic vesicle endocytosis	ISS Inferred from Sequence or Structural Similarity more info
involved_in viral protein processing	TAS Traceable Author Statement more info

Component	Evidence Code	Pubs
located_in endoplasmic reticulum	IDA Inferred from Direct Assay more info	PubMed
located_in endoplasmic reticulum lumen	TAS Traceable Author Statement more info
is_active_in endoplasmic reticulum membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in endoplasmic reticulum membrane	IDA Inferred from Direct Assay more info	PubMed
located_in endoplasmic reticulum quality control compartment	IEA Inferred from Electronic Annotation more info
located_in extracellular exosome	HDA more info	PubMed
NOT located_in extracellular exosome	IDA Inferred from Direct Assay more info	PubMed
located_in lumenal side of endoplasmic reticulum membrane	TAS Traceable Author Statement more info
located_in melanosome membrane	IEA Inferred from Electronic Annotation more info
located_in membrane	HDA more info	PubMed
located_in mitochondria-associated endoplasmic reticulum membrane contact site	IDA Inferred from Direct Assay more info	PubMed
located_in mitochondrial membrane	IEA Inferred from Electronic Annotation more info
located_in nuclear membrane	IEA Inferred from Electronic Annotation more info
located_in presynapse	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: calnexin

Names: epididymis secretory sperm binding protein; major histocompatibility complex class I antigen-binding protein p88

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001024649.2 → NP_001019820.1 calnexin isoform d precursor

See identical proteins and their annotated locations for NP_001019820.1

Status: REVIEWED

Source sequence(s)

AC113426, BC042843

Consensus CDS

CCDS4447.1

UniProtKB/Swiss-Prot

B2R5V8, B4DGP8, B4E2T8, D3DWQ3, D6R9K3, P27824

UniProtKB/TrEMBL

A0A7P0T840

Related

ENSP00000391646.2, ENST00000452673.6

Conserved Domains (1) summary

pfam00262
Location:71 → 440

Calreticulin; Calreticulin family
NM_001363993.1 → NP_001350922.1 calnexin isoform a

Status: REVIEWED

Description

Transcript Variant: This variant (3) represents the longest transcript and encodes the longest isoform (a).

Source sequence(s)

AC113426

UniProtKB/TrEMBL

A0A7P0T840

Conserved Domains (1) summary

pfam00262
Location:125 → 494

Calreticulin; Calreticulin family
NM_001363994.1 → NP_001350923.1 calnexin isoform b precursor

Status: REVIEWED

Source sequence(s)

AC113426, AK294702

UniProtKB/TrEMBL

A0A7P0T840

Conserved Domains (1) summary

pfam00262
Location:106 → 475

Calreticulin; Calreticulin family
NM_001363995.1 → NP_001350924.1 calnexin isoform c precursor

Status: REVIEWED

Source sequence(s)

AC113426

Consensus CDS

CCDS93837.1

UniProtKB/TrEMBL

A0A7P0T840, A0A7P0TAE9

Related

ENSP00000506168.1, ENST00000680984.1

Conserved Domains (1) summary

pfam00262
Location:71 → 474

Calreticulin; Calreticulin family
NM_001363996.1 → NP_001350925.1 calnexin isoform d precursor

Status: REVIEWED

Source sequence(s)

AC113426

Consensus CDS

CCDS4447.1

UniProtKB/Swiss-Prot

B2R5V8, B4DGP8, B4E2T8, D3DWQ3, D6R9K3, P27824

UniProtKB/TrEMBL

A0A7P0T840

Related

ENSP00000424745.2, ENST00000502296.6

Conserved Domains (1) summary

pfam00262
Location:71 → 440

Calreticulin; Calreticulin family
NM_001363997.1 → NP_001350926.1 calnexin isoform d precursor

Status: REVIEWED

Source sequence(s)

AC113426

Consensus CDS

CCDS4447.1

UniProtKB/Swiss-Prot

B2R5V8, B4DGP8, B4E2T8, D3DWQ3, D6R9K3, P27824

UniProtKB/TrEMBL

A0A7P0T840

Related

ENSP00000506003.1, ENST00000681476.1

Conserved Domains (1) summary

pfam00262
Location:71 → 440

Calreticulin; Calreticulin family
NM_001363998.1 → NP_001350927.1 calnexin isoform e precursor

Status: REVIEWED

Source sequence(s)

AC113426

Consensus CDS

CCDS93838.1

UniProtKB/TrEMBL

A0A7P0T840, A0A7P0T937

Related

ENSP00000505470.1, ENST00000680894.1

Conserved Domains (1) summary

pfam00262
Location:71 → 440

Calreticulin; Calreticulin family
NM_001363999.1 → NP_001350928.1 calnexin isoform f precursor

Status: REVIEWED

Source sequence(s)

AC113426

Consensus CDS

CCDS93839.1

UniProtKB/TrEMBL

A0A7P0T840, A0A7P0TB65

Related

ENSP00000506591.1, ENST00000681894.1

Conserved Domains (1) summary

pfam00262
Location:71 → 440

Calreticulin; Calreticulin family
NM_001364000.1 → NP_001350929.1 calnexin isoform g

Status: REVIEWED

Source sequence(s)

AC113426, AK304420

Consensus CDS

CCDS93840.1

UniProtKB/TrEMBL

D6RGY2

Related

ENSP00000505129.1, ENST00000680006.1

Conserved Domains (1) summary

pfam00262
Location:6 → 332

Calreticulin; Calreticulin family
NM_001364001.1 → NP_001350930.1 calnexin isoform g

Status: REVIEWED

Source sequence(s)

AC113426

Consensus CDS

CCDS93840.1

UniProtKB/TrEMBL

D6RGY2

Related

ENSP00000506051.1, ENST00000680827.1

Conserved Domains (1) summary

pfam00262
Location:6 → 332

Calreticulin; Calreticulin family
NM_001746.4 → NP_001737.1 calnexin isoform d precursor

See identical proteins and their annotated locations for NP_001737.1

Status: REVIEWED

Source sequence(s)

AC113426, BC003552

Consensus CDS

CCDS4447.1

UniProtKB/Swiss-Prot

B2R5V8, B4DGP8, B4E2T8, D3DWQ3, D6R9K3, P27824

UniProtKB/TrEMBL

A0A7P0T840

Related

ENSP00000247461.4, ENST00000247461.9

Conserved Domains (1) summary

pfam00262
Location:71 → 440

Calreticulin; Calreticulin family