Capn1 calpain 1 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Capn1provided by MGI
Official Full Name: calpain 1provided by MGI
Primary source: MGI:MGI:88263
See related: Ensembl:ENSMUSG00000024942 AllianceGenome:MGI:88263
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: Capa1; Capa-1; mu-calpin
Summary: Enables calcium-dependent cysteine-type endopeptidase activity. Acts upstream of or within mammary gland involution; proteolysis; and receptor catabolic process. Located in cytosol; lysosome; and mitochondrion. Is active in cornified envelope. Is expressed in liver; molar; oral region epithelium; and skin. Human ortholog(s) of this gene implicated in hereditary spastic paraplegia 76. Orthologous to human CAPN1 (calpain 1). [provided by Alliance of Genome Resources, Oct 2024]
Expression: Ubiquitous expression in large intestine adult (RPKM 48.9), bladder adult (RPKM 44.0) and 24 other tissues See more
Orthologs: human all
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Genomic context

Location:: 19 A; 19 4.34 cM

Exon count:: 23

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	19	NC_000085.7 (6038573..6065855, complement)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	19	NC_000085.6 (5988545..6015825, complement)

Chromosome 19 - NC_000085.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Hydrogen-rich saline alleviates cardiomyocyte apoptosis by reducing expression of calpain1 via miR-124-3p.
Title: Hydrogen-rich saline alleviates cardiomyocyte apoptosis by reducing expression of calpain1 via miR-124-3p.
Calpain-1 mediates vascular remodelling and fibrosis via HIF-1alpha in hypoxia-induced pulmonary hypertension.
Title: Calpain-1 mediates vascular remodelling and fibrosis via HIF-1α in hypoxia-induced pulmonary hypertension.
Exosome-mediated improvement in membrane integrity and muscle function in dystrophic mice.
Title: Exosome-mediated improvement in membrane integrity and muscle function in dystrophic mice.
miR1405p alleviates mouse liver ischemia/reperfusion injury by targeting CAPN1.
Title: miR‑140‑5p alleviates mouse liver ischemia/reperfusion injury by targeting CAPN1.
Platelet-derived calpain cleaves the endothelial protease-activated receptor 1 to induce vascular inflammation in diabetes.
Title: Platelet-derived calpain cleaves the endothelial protease-activated receptor 1 to induce vascular inflammation in diabetes.
Photoreceptor Cell Calcium Dysregulation and Calpain Activation Promote Pathogenic Photoreceptor Oxidative Stress and Inflammation in Prodromal Diabetic Retinopathy.
Title: Photoreceptor Cell Calcium Dysregulation and Calpain Activation Promote Pathogenic Photoreceptor Oxidative Stress and Inflammation in Prodromal Diabetic Retinopathy.
Exercise triggers CAPN1-mediated AIF truncation, inducing myocyte cell death in arrhythmogenic cardiomyopathy.
Title: Exercise triggers CAPN1-mediated AIF truncation, inducing myocyte cell death in arrhythmogenic cardiomyopathy.
Calpain-1 ablation partially rescues disease-associated hallmarks in models of Machado-Joseph disease.
Title: Calpain-1 ablation partially rescues disease-associated hallmarks in models of Machado-Joseph disease.
Impaired cerebellar plasticity and eye-blink conditioning in calpain-1 knock-out mice.
Title: Impaired cerebellar plasticity and eye-blink conditioning in calpain-1 knock-out mice.
Inhibition of the ERK1/2-ubiquitous calpains pathway attenuates experimental pulmonary fibrosis in vivo and in vitro.
Title: Inhibition of the ERK1/2-ubiquitous calpains pathway attenuates experimental pulmonary fibrosis in vivo and in vitro.

Submit: New GeneRIF Correction See all GeneRIFs (129)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Endonuclease-mediated (2)
Targeted (3) 1 citation

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

AF021847 (e-PCR)
- UniSTS:160777

MARC_4751-4752:996679637:1 (e-PCR)
- UniSTS:269428

Capn1 (e-PCR), detects polymorphism
- UniSTS:141223

RH128571 (e-PCR)
- UniSTS:211879

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables calcium ion binding	ISO Inferred from Sequence Orthology more info
enables calcium ion binding	ISS Inferred from Sequence or Structural Similarity more info
enables calcium-dependent cysteine-type endopeptidase activity	IBA Inferred from Biological aspect of Ancestor more info
enables calcium-dependent cysteine-type endopeptidase activity	IDA Inferred from Direct Assay more info	PubMed
enables calcium-dependent cysteine-type endopeptidase activity	ISO Inferred from Sequence Orthology more info	PubMed
enables calcium-dependent cysteine-type endopeptidase activity	TAS Traceable Author Statement more info	PubMed
enables cytoskeletal protein binding	ISO Inferred from Sequence Orthology more info
enables enzyme binding	ISO Inferred from Sequence Orthology more info
enables peptidase activity	ISO Inferred from Sequence Orthology more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
involved_in cellular response to hydrogen peroxide	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within mammary gland involution	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of actin filament polymerization	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of non-canonical NF-kappaB signal transduction	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of cardiac muscle cell apoptotic process	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of leukocyte tethering or rolling	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of vascular permeability	ISO Inferred from Sequence Orthology more info
involved_in protein autoprocessing	ISO Inferred from Sequence Orthology more info
involved_in protein catabolic process	ISO Inferred from Sequence Orthology more info
involved_in proteolysis	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within proteolysis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in proteolysis	ISO Inferred from Sequence Orthology more info
involved_in proteolysis	ISS Inferred from Sequence or Structural Similarity more info
involved_in proteolysis	NAS Non-traceable Author Statement more info	PubMed
acts_upstream_of_or_within receptor catabolic process	IDA Inferred from Direct Assay more info	PubMed
involved_in regulation of catalytic activity	ISO Inferred from Sequence Orthology more info
involved_in regulation of catalytic activity	ISS Inferred from Sequence or Structural Similarity more info
involved_in response to angiotensin	ISO Inferred from Sequence Orthology more info
involved_in self proteolysis	ISO Inferred from Sequence Orthology more info
involved_in self proteolysis	ISS Inferred from Sequence or Structural Similarity more info

Component	Evidence Code	Pubs
part_of calpain complex	ISS Inferred from Sequence or Structural Similarity more info
is_active_in cornified envelope	IDA Inferred from Direct Assay more info	PubMed
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
located_in cytosol	IDA Inferred from Direct Assay more info	PubMed
located_in cytosol	ISO Inferred from Sequence Orthology more info
located_in cytosol	ISS Inferred from Sequence or Structural Similarity more info
located_in lysosome	IDA Inferred from Direct Assay more info	PubMed
located_in membrane	ISO Inferred from Sequence Orthology more info
located_in mitochondrion	IDA Inferred from Direct Assay more info	PubMed
located_in plasma membrane	ISO Inferred from Sequence Orthology more info
located_in plasma membrane	ISS Inferred from Sequence or Structural Similarity more info
located_in plasma membrane	TAS Traceable Author Statement more info

General protein information

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Preferred Names: calpain-1 catalytic subunit

Names: CANP 1; calcium-activated neutral proteinase 1; calpain mu-type; calpain-1 large subunit; micromolar-calpain; mu-calpain; muCANP

NP_001103974.1

EC 3.4.22.52

NP_031626.1

EC 3.4.22.52

XP_036017307.1

EC 3.4.22.52

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001110504.2 → NP_001103974.1 calpain-1 catalytic subunit

See identical proteins and their annotated locations for NP_001103974.1

Status: VALIDATED

Source sequence(s)

AC131692

Consensus CDS

CCDS37893.1

UniProtKB/Swiss-Prot

O35350, O88666

UniProtKB/TrEMBL

Q3TI07, Q3UDG8, Q80Y25, Q8C2J1

Related

ENSMUSP00000127498.2, ENSMUST00000164843.10

Conserved Domains (6) summary

PTZ00184
Location:537 → 680

PTZ00184; calmodulin; Provisional

cd00051
Location:589 → 643

EFh; EF-hand, calcium binding motif; A diverse superfamily of calcium sensors and calcium signal modulators; most examples in this alignment model have 2 active canonical EF hands. Ca2+ binding induces a conformational change in the EF-hand motif, leading to ...

pfam00648
Location:56 → 352

Peptidase_C2; Calpain family cysteine protease

pfam01067
Location:366 → 518

Calpain_III; Calpain large subunit, domain III

pfam13833
Location:556 → 609

EF-hand_8; EF-hand domain pair

cl08302
Location:622 → 704

EFh; EF-hand, calcium binding motif; A diverse superfamily of calcium sensors and calcium signal modulators; most examples in this alignment model have 2 active canonical EF hands. Ca2+ binding induces a conformational change in the EF-hand motif, leading to ...
NM_007600.4 → NP_031626.1 calpain-1 catalytic subunit

See identical proteins and their annotated locations for NP_031626.1

Status: VALIDATED

Source sequence(s)

AC131692

Consensus CDS

CCDS37893.1

UniProtKB/Swiss-Prot

O35350, O88666

UniProtKB/TrEMBL

Q3TI07, Q3UDG8, Q80Y25, Q8C2J1

Related

ENSMUSP00000025891.9, ENSMUST00000025891.11

Conserved Domains (6) summary

PTZ00184
Location:537 → 680

PTZ00184; calmodulin; Provisional

cd00051
Location:589 → 643

EFh; EF-hand, calcium binding motif; A diverse superfamily of calcium sensors and calcium signal modulators; most examples in this alignment model have 2 active canonical EF hands. Ca2+ binding induces a conformational change in the EF-hand motif, leading to ...

pfam00648
Location:56 → 352

Peptidase_C2; Calpain family cysteine protease

pfam01067
Location:366 → 518

Calpain_III; Calpain large subunit, domain III

pfam13833
Location:556 → 609

EF-hand_8; EF-hand domain pair

cl08302
Location:622 → 704

EFh; EF-hand, calcium binding motif; A diverse superfamily of calcium sensors and calcium signal modulators; most examples in this alignment model have 2 active canonical EF hands. Ca2+ binding induces a conformational change in the EF-hand motif, leading to ...

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000085.7 Reference GRCm39 C57BL/6J

Range

6038573..6065855 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_036161414.1 → XP_036017307.1 calpain-1 catalytic subunit isoform X1

UniProtKB/TrEMBL

Q3TB79

Conserved Domains (4) summary

cd00214
Location:366 → 523

Calpain_III; Calpain, subdomain III. Calpains are calcium-activated cytoplasmic cysteine proteinases, participate in cytoskeletal remodeling processes, cell differentiation, apoptosis and signal transduction. Catalytic domain and the two calmodulin-like domains are ...

cd15897
Location:545 → 573

EFh_PEF; EF-hand motif [structural motif]

cl25352
Location:545 → 647

EFh_PEF; The penta-EF hand (PEF) family

pfam00648
Location:56 → 352

Peptidase_C2; Calpain family cysteine protease