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    Ube2k ubiquitin-conjugating enzyme E2K [ Mus musculus (house mouse) ]

    Gene ID: 53323, updated on 28-Oct-2024

    Summary

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    Official Symbol
    Ube2kprovided by MGI
    Official Full Name
    ubiquitin-conjugating enzyme E2Kprovided by MGI
    Primary source
    MGI:MGI:1858216
    See related
    Ensembl:ENSMUSG00000029203 AllianceGenome:MGI:1858216
    Gene type
    protein coding
    RefSeq status
    VALIDATED
    Organism
    Mus musculus
    Lineage
    Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
    Also known as
    Lig; Hip2; Hypg; HIP-2; E2-25k; D5Ertd601e
    Summary
    Predicted to enable ubiquitin conjugating enzyme activity; ubiquitin protein ligase binding activity; and ubiquitin-ubiquitin ligase activity. Acts upstream of or within regulation of proteasomal ubiquitin-dependent protein catabolic process. Located in cytoplasm; filopodium tip; and nucleus. Is expressed in several structures, including alimentary system; brain; genitourinary system; respiratory system; and sensory organ. Orthologous to human UBE2K (ubiquitin conjugating enzyme E2 K). [provided by Alliance of Genome Resources, Oct 2024]
    Expression
    Ubiquitous expression in testis adult (RPKM 20.5), CNS E18 (RPKM 19.6) and 28 other tissues See more
    Orthologs
    human all
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    Genomic context

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    See Ube2k in Genome Data Viewer
    Location:
    5 C3.1; 5 33.72 cM
    Exon count:
    7
    Annotation release Status Assembly Chr Location
    RS_2024_02 current GRCm39 (GCF_000001635.27) 5 NC_000071.7 (65694588..65756332)
    108.20200622 previous assembly GRCm38.p6 (GCF_000001635.26) 5 NC_000071.6 (65537245..65598989)

    Chromosome 5 - NC_000071.7Genomic Context describing neighboring genes Neighboring gene VISTA enhancer mm1569 Neighboring gene STARR-seq mESC enhancer starr_13263 Neighboring gene STARR-positive B cell enhancer ABC_E4757 Neighboring gene UDP-glucose dehydrogenase Neighboring gene small integral membrane protein 14 Neighboring gene STARR-positive B cell enhancer ABC_E4758 Neighboring gene STARR-seq mESC enhancer starr_13264 Neighboring gene STARR-positive B cell enhancer ABC_E4759 Neighboring gene STARR-positive B cell enhancer ABC_E8059 Neighboring gene STARR-positive B cell enhancer ABC_E11290 Neighboring gene predicted gene, 40309 Neighboring gene PDS5 cohesin associated factor A Neighboring gene STARR-seq mESC enhancer starr_13267 Neighboring gene predicted gene, 24399 Neighboring gene STARR-positive B cell enhancer ABC_E3578 Neighboring gene STARR-seq mESC enhancer starr_13273 Neighboring gene CapStarr-seq enhancer MGSCv37_chr5:66156164-66156347 Neighboring gene predicted gene 5296 Neighboring gene NEDD4 binding protein 2

    Genomic regions, transcripts, and products

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    Go to nucleotide: Graphics FASTA GenBank

    Expression

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    • Project title: Mouse ENCODE transcriptome data Mouse ENCODE transcriptome data
    • Description: RNA profiling data sets generated by the Mouse ENCODE project.
    • BioProject: PRJNA66167
    • Publication: PMID 25409824
    • Analysis date: n/a

    Bibliography

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    Related articles in PubMed

    1. E2-25K SUMOylation inhibits proteasome for cell death during cerebral ischemia/reperfusion. Jeong EI, et al. Cell Death Dis, 2016 Dec 29. PMID 28032866, Free PMC Article
    2. Localization of huntingtin-interacting protein-2 (Hip-2) mRNA in the developing mouse brain. Tanno Y, et al. J Chem Neuroanat, 1999 Oct. PMID 10585161
    3. UBE2K regulated by IGF2BP3 promotes cell proliferation and stemness in pancreatic ductal adenocarcinoma. Fu W, et al. Int J Oncol, 2023 Apr. PMID 36896783, Free PMC Article
    4. Reduction of HIP2 expression causes motor function impairment and increased vulnerability to dopaminergic degeneration in Parkinson's disease models. Su J, et al. Cell Death Dis, 2018 Oct 3. PMID 30282965, Free PMC Article
    5. E2-25K/Hip-2 regulates caspase-12 in ER stress-mediated Abeta neurotoxicity. Song S, et al. J Cell Biol, 2008 Aug 25. PMID 18710920, Free PMC Article

    See all (37) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. UBE2K regulated by IGF2BP3 promotes cell proliferation and stemness in pancreatic ductal adenocarcinoma.
      Title: UBE2K regulated by IGF2BP3 promotes cell proliferation and stemness in pancreatic ductal adenocarcinoma.
    2. Immunohistochemical Expression Pattern of FGFR1, FGFR2, RIP5, and HIP2 in Developing and Postnatal Kidneys of Dab1(-/-) (yotari) Mice.
      Title: Immunohistochemical Expression Pattern of FGFR1, FGFR2, RIP5, and HIP2 in Developing and Postnatal Kidneys of Dab1(-/-) (yotari) Mice.
    3. HIP2 insufficiency as a contributing factor for PD.
      Title: Reduction of HIP2 expression causes motor function impairment and increased vulnerability to dopaminergic degeneration in Parkinson's disease models.
    4. E2-25K has a crucial role in oxidative stress and cerebral Ischemia/Reperfusion-induced damage through inhibiting proteasome via its SUMOylation.
      Title: E2-25K SUMOylation inhibits proteasome for cell death during cerebral ischemia/reperfusion.
    5. E2-25K/Hip-2 is thus an essential upstream regulator of the expression and activation of caspase-12 in endoplasmic reticulum stress-mediated Abeta neurotoxicity.
      Title: E2-25K/Hip-2 regulates caspase-12 in ER stress-mediated Abeta neurotoxicity.
    Submit: New GeneRIF Correction

    Variation

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    Alleles

    Alleles of this type are documented at Mouse Genome Informatics  (MGI)
    • Endonuclease-mediated (6) 
    • Gene trapped (1)  1 citation

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Markers

    Homology

    Gene Ontology Provided by MGI

    Function Evidence Code Pubs
    enables ATP binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables ubiquitin conjugating enzyme activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables ubiquitin conjugating enzyme activity ISO
    Inferred from Sequence Orthology
    more info
    		  
    enables ubiquitin conjugating enzyme activity TAS
    Traceable Author Statement
    more info
    		 PubMed 
    enables ubiquitin protein ligase binding ISO
    Inferred from Sequence Orthology
    more info
    		  
    enables ubiquitin protein ligase binding ISS
    Inferred from Sequence or Structural Similarity
    more info
    		  
    enables ubiquitin-protein transferase activity ISO
    Inferred from Sequence Orthology
    more info
    		  
    enables ubiquitin-protein transferase activity ISS
    Inferred from Sequence or Structural Similarity
    more info
    		  
    enables ubiquitin-ubiquitin ligase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables ubiquitin-ubiquitin ligase activity ISO
    Inferred from Sequence Orthology
    more info
    		  
    Process Evidence Code Pubs
    involved_in cellular response to interferon-beta IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in cellular response to interferon-beta ISO
    Inferred from Sequence Orthology
    more info
    		  
    involved_in free ubiquitin chain polymerization IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in free ubiquitin chain polymerization ISO
    Inferred from Sequence Orthology
    more info
    		  
    acts_upstream_of_or_within intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress ISO
    Inferred from Sequence Orthology
    more info
    		  
    involved_in positive regulation of peptidyl-threonine phosphorylation IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in positive regulation of peptidyl-threonine phosphorylation ISO
    Inferred from Sequence Orthology
    more info
    		  
    involved_in positive regulation of tumor necrosis factor-mediated signaling pathway IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in positive regulation of tumor necrosis factor-mediated signaling pathway ISO
    Inferred from Sequence Orthology
    more info
    		  
    involved_in positive regulation of type I interferon-mediated signaling pathway IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in positive regulation of type I interferon-mediated signaling pathway ISO
    Inferred from Sequence Orthology
    more info
    		  
    involved_in protein K48-linked ubiquitination ISO
    Inferred from Sequence Orthology
    more info
    		  
    involved_in protein K48-linked ubiquitination ISS
    Inferred from Sequence or Structural Similarity
    more info
    		  
    involved_in protein polyubiquitination IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    acts_upstream_of_or_within regulation of proteasomal ubiquitin-dependent protein catabolic process IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    Component Evidence Code Pubs
    located_in cytoplasm IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in filopodium tip IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    is_active_in nucleus IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in nucleus IDA
    Inferred from Direct Assay
    more info
    		 PubMed 

    General protein information

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    Preferred Names
    ubiquitin-conjugating enzyme E2 K
    Names
    E2 ubiquitin-conjugating enzyme K
    huntingtin interacting protein 2
    ubiquitin carrier protein
    ubiquitin-conjugating enzyme E2-25 kDa
    ubiquitin-conjugating enzyme E2K (UBC1 homolog, yeast)
    ubiquitin-protein ligase
    NP_001297547.1
    NP_001297548.1
    NP_058066.2

    NCBI Reference Sequences (RefSeq)

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    RefSeqs maintained independently of Annotated Genomes

    These reference sequences exist independently of genome builds. Explain

    These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

    mRNA and Protein(s)

    1. NM_001310618.1 → NP_001297547.1  ubiquitin-conjugating enzyme E2 K isoform 2

      See identical proteins and their annotated locations for NP_001297547.1

      Status: VALIDATED

      Description
      Transcript Variant: This variant (2) lacks two alternate in-frame exons compared to variant 1. The encoded isoform (2) is shorter than isoform 1.
      Source sequence(s)
      AK036068, AK139564, AK149463, CF538783
      Consensus CDS
      CCDS80289.1
      UniProtKB/TrEMBL
      A0A0J9YU07
      Related
      ENSMUSP00000143941.2, ENSMUST00000201984.4
      Conserved Domains (2) summary
      smart00212
      Location:22 → 103
      UBCc; Ubiquitin-conjugating enzyme E2, catalytic domain homologues
      cl21463
      Location:112 → 149
      UBA_like_SF; UBA domain-like superfamily

    2. NM_001310619.1 → NP_001297548.1  ubiquitin-conjugating enzyme E2 K isoform 3

      Status: VALIDATED

      Description
      Transcript Variant: This variant (3) lacks an exon in the central coding region and initiates translation at an alternate start codon, compared to variant 1. The encoded isoform (3) has a distinct, shorter N-terminus than isoform 1.
      Source sequence(s)
      AC112263, AC136513
      Conserved Domains (2) summary
      smart00212
      Location:2 → 84
      UBCc; Ubiquitin-conjugating enzyme E2, catalytic domain homologues
      cl21463
      Location:93 → 130
      UBA_like_SF; UBA domain-like superfamily

    3. NM_016786.4 → NP_058066.2  ubiquitin-conjugating enzyme E2 K isoform 1

      See identical proteins and their annotated locations for NP_058066.2

      Status: VALIDATED

      Description
      Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1).
      Source sequence(s)
      AK036068, AK139564, AK149463
      Consensus CDS
      CCDS39098.1
      UniProtKB/Swiss-Prot
      P61087
      UniProtKB/TrEMBL
      Q3TL77, Q6ZWQ6
      Related
      ENSMUSP00000122471.2, ENSMUST00000142407.8
      Conserved Domains (2) summary
      cd00195
      Location:6 → 149
      UBCc; Ubiquitin-conjugating enzyme E2, catalytic (UBCc) domain. This is part of the ubiquitin-mediated protein degradation pathway in which a thiol-ester linkage forms between a conserved cysteine and the C-terminus of ubiquitin and complexes with ubiquitin ...
      cd14390
      Location:163 → 200
      UBA_II_E2_UBE2K; UBA domain of vertebrate ubiquitin-conjugating enzyme E2 K (UBE2K)

    RNA

    1. NR_144566.1 RNA Sequence

      Status: VALIDATED

      Description
      Transcript Variant: This variant (4) lacks an alternate exon compared to variant 1. This variant is represented as non-coding because the predicted protein does not meet RefSeq quality criteria.
      Source sequence(s)
      AC112263, AC136513
      Related
      ENSMUST00000202679.4

    RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference GRCm39 C57BL/6J

    Genomic

    1. NC_000071.7 Reference GRCm39 C57BL/6J

      Range
      65694588..65756332
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)
    Nucleotide Protein Strain
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    P61087.3 GenPept UniProtKB/Swiss-Prot:P61087

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