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    Tpm3 tropomyosin 3, gamma [ Mus musculus (house mouse) ]

    Gene ID: 59069, updated on 2-Nov-2024

    Summary

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    Official Symbol
    Tpm3provided by MGI
    Official Full Name
    tropomyosin 3, gammaprovided by MGI
    Primary source
    MGI:MGI:1890149
    See related
    Ensembl:ENSMUSG00000027940 AllianceGenome:MGI:1890149
    Gene type
    protein coding
    RefSeq status
    VALIDATED
    Organism
    Mus musculus
    Lineage
    Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
    Also known as
    TMnm; Tpm5; Tm5NM; Tpm-5; hTMnm; TM30nm; Trop-5; hTM30nm; gamma-TM
    Summary
    Enables actin filament binding activity. Predicted to be involved in actin filament organization and muscle contraction. Located in cleavage furrow; cytoskeleton; and growth cone. Is expressed in coelomic epithelium of testis; ovary; and primary sex cord. Human ortholog(s) of this gene implicated in congenital myopathy 4A and nemaline myopathy 1. Orthologous to human TPM3 (tropomyosin 3). [provided by Alliance of Genome Resources, Nov 2024]
    Expression
    Ubiquitous expression in placenta adult (RPKM 17.7), CNS E11.5 (RPKM 17.1) and 28 other tissues See more
    Orthologs
    human all
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    Genomic context

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    See Tpm3 in Genome Data Viewer
    Location:
    3 F1; 3 39.2 cM
    Exon count:
    14
    Annotation release Status Assembly Chr Location
    RS_2024_02 current GRCm39 (GCF_000001635.27) 3 NC_000069.7 (89979958..90008209)
    108.20200622 previous assembly GRCm38.p6 (GCF_000001635.26) 3 NC_000069.6 (90072651..90100902)

    Chromosome 3 - NC_000069.7Genomic Context describing neighboring genes Neighboring gene cilia and flagella associated protein 141 Neighboring gene STARR-seq mESC enhancer starr_08271 Neighboring gene STARR-positive B cell enhancer ABC_E3447 Neighboring gene STARR-positive B cell enhancer ABC_E7923 Neighboring gene microRNA 190b Neighboring gene STARR-seq mESC enhancer starr_08272 Neighboring gene nucleoporin 210-like Neighboring gene predicted gene, 34865 Neighboring gene predicted gene, 23723 Neighboring gene STARR-seq mESC enhancer starr_08276 Neighboring gene predicted gene, 24185

    Genomic regions, transcripts, and products

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    Go to nucleotide: Graphics FASTA GenBank

    Expression

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    • Project title: Mouse ENCODE transcriptome data Mouse ENCODE transcriptome data
    • Description: RNA profiling data sets generated by the Mouse ENCODE project.
    • BioProject: PRJNA66167
    • Publication: PMID 25409824
    • Analysis date: n/a

    Bibliography

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    Related articles in PubMed

    1. Cytoskeletal tropomyosin Tm5NM1 is required for normal excitation-contraction coupling in skeletal muscle. Vlahovich N, et al. Mol Biol Cell, 2009 Jan. PMID 19005216, Free PMC Article
    2. Nucleotide sequence of cDNA for nonmuscle tropomyosin 5 of mouse fibroblast. Takenaga K, et al. Biochim Biophys Acta, 1990 Sep 10. PMID 2400784
    3. Differential regulation of tropomyosin isoform organization and gene expression in response to altered actin gene expression. Schevzov G, et al. J Cell Biol, 1993 May. PMID 8491774, Free PMC Article
    4. Tropomyosin 3 increases striated muscle isoform diversity. Pieples K, et al. Biochemistry, 2000 Jul 18. PMID 10889038
    5. Tropomyosin 3 expression leads to hypercontractility and attenuates myofilament length-dependent Ca(2+) activation. Pieples K, et al. Am J Physiol Heart Circ Physiol, 2002 Oct. PMID 12234784

    See all (69) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Deletion of the Actin-Associated Tropomyosin Tpm3 Leads to Reduced Cell Complexity in Cultured Hippocampal Neurons-New Insights into the Role of the C-Terminal Region of Tpm3.1.
      Title: Deletion of the Actin-Associated Tropomyosin Tpm3 Leads to Reduced Cell Complexity in Cultured Hippocampal Neurons-New Insights into the Role of the C-Terminal Region of Tpm3.1.
    2. Tpm3.1 co-assembles onto these actin filaments, and this is followed later by recruitment of the myosin IIA motor.
      Title: Parallel assembly of actin and tropomyosin, but not myosin II, during de novo actin filament formation in live mice.
    3. The role of TPM3.1 protein isoform in cell proliferation
      Title: Ras Transformation Overrides a Proliferation Defect Induced by Tpm3.1 Knockout.
    4. Tpm3 plays crucial roles in maintaining cortical actin integrity and asymmetric cell division during oocyte maturation, and that dynamic regulation of cortical actin by Tpm3 is critical to ensure proper polar body protrusion.
      Title: Non-muscle tropomyosin (Tpm3) is crucial for asymmetric cell division and maintenance of cortical integrity in mouse oocytes.
    5. TMBr-3 and TM5NM1 modestly affected actin assembly kinetics, in an opposite manner.
      Title: Purification of tropomyosin Br-3 and 5NM1 and characterization of their interactions with actin.
    6. Tropomyosin regulates cell migration during skin wound healing.
      Title: Tropomyosin regulates cell migration during skin wound healing.
    7. Data conclude that exon 9d-containing isoforms are not essential for survival of neuronal cells and that isoform choice from the gamma-Tm gene is flexible in the brain
      Title: New aspects of tropomyosin-regulated neuritogenesis revealed by the deletion of Tm5NM1 and 2.
    8. Reduced expression of Tpm3, sarcomere genes and Lrtm1 in the diaphragm of mice with diaphragmatic defects.
      Title: Examination of FGFRL1 as a candidate gene for diaphragmatic defects at chromosome 4p16.3 shows that Fgfrl1 null mice have reduced expression of Tpm3, sarcomere genes and Lrtm1 in the diaphragm.
    9. The expression of an inappropriate Tm in skeletal muscle results in increased susceptibility to contraction-induced damage
      Title: A cytoskeletal tropomyosin can compromise the structural integrity of skeletal muscle.
    10. results indicate that a Tm5NM1-defined actin cytoskeleton is required for the normal excitation-contraction coupling in skeletal muscle
      Title: Cytoskeletal tropomyosin Tm5NM1 is required for normal excitation-contraction coupling in skeletal muscle.
    Submit: New GeneRIF Correction See all GeneRIFs (15)

    Variation

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    Alleles

    Alleles of this type are documented at Mouse Genome Informatics  (MGI)
    • Endonuclease-mediated (1) 
    • Gene trapped (1) 
    • Targeted (5)  1 citation

    Pathways from PubChem

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    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Markers

    Homology

    Clone Names

    • MGC102590

    Gene Ontology Provided by MGI

    Function Evidence Code Pubs
    enables actin filament binding IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables actin filament binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    Process Evidence Code Pubs
    involved_in actin filament organization IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in muscle contraction IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    Component Evidence Code Pubs
    located_in actin cytoskeleton ISO
    Inferred from Sequence Orthology
    more info
    		  
    is_active_in actin filament IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in cleavage furrow IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in cortical cytoskeleton IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in cytoplasm IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in cytosol ISO
    Inferred from Sequence Orthology
    more info
    		  
    located_in growth cone IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in neuron projection IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in podosome IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in stress fiber ISO
    Inferred from Sequence Orthology
    more info
    		 PubMed 

    General protein information

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    Preferred Names
    tropomyosin alpha-3 chain
    Names
    gamma-tropomyosin
    skalphaTM.2
    tropomyosin 5
    tropomyosin-3

    NCBI Reference Sequences (RefSeq)

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    RefSeqs maintained independently of Annotated Genomes

    These reference sequences exist independently of genome builds. Explain

    These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

    mRNA and Protein(s)

    1. NM_001253738.1 → NP_001240667.1  tropomyosin alpha-3 chain isoform Tpm3.1cy

      See identical proteins and their annotated locations for NP_001240667.1

      Status: VALIDATED

      Description
      Transcript Variant: This variant (3, also known as variant Tpm3.1) represents the use of an alternate promoter and differs in the 5' structure, causing translation initiation at an alternate start codon compared to variant Tpm3.13. The encoded isoform (Tpm3.1cy, also known as isoform 3) has a distinct N-terminus and is shorter than isoform Tpm3.13cy. Isoforms Tpm3.1cy, Tpm3.2cy, and Tpm3.5cy are the same length, but contain distinct C-termini.
      Source sequence(s)
      AK146557, BY284672, BY622941
      Consensus CDS
      CCDS57223.1
      UniProtKB/TrEMBL
      D3Z2H9, Q58E70
      Related
      ENSMUSP00000029549.9, ENSMUST00000029549.16
      Conserved Domains (1) summary
      pfam00261
      Location:12 → 246
      Tropomyosin; Tropomyosin

    2. NM_001253740.1 → NP_001240669.1  tropomyosin alpha-3 chain isoform Tpm3.2cy

      See identical proteins and their annotated locations for NP_001240669.1

      Status: VALIDATED

      Description
      Transcript Variant: This variant (4, also known as variant Tpm3.2) represents the use of an alternate promoter and has multiple differences compared to variant Tpm3.13. These differences result in a different 5' UTR and cause translation initiation at an alternate start codon compared to variant Tpm3.13. The encoded isoform (Tpm3.2cy, also known as isoform 4) has a distinct N-terminus and is shorter than isoform Tpm3.13cy. Isoforms Tpm3.1cy, Tpm3.2cy, and Tpm3.5cy are the same length, but contain distinct C-termini.
      Source sequence(s)
      AK146557, BY284672, BY622941, CX237327
      UniProtKB/TrEMBL
      Q58E70
      Conserved Domains (2) summary
      pfam00261
      Location:12 → 246
      Tropomyosin; Tropomyosin
      pfam06009
      Location:150 → 229
      Laminin_II; Laminin Domain II

    3. NM_001271764.2 → NP_001258693.1  tropomyosin alpha-3 chain isoform Tpm3.5cy

      See identical proteins and their annotated locations for NP_001258693.1

      Status: VALIDATED

      Description
      Transcript Variant: This variant (5, also known as variant Tpm3.5) represents the use of an alternate promoter and has multiple differences compared to variant Tpm3.13. These differences result in different 5' and 3' UTRs and cause translation initiation at an alternate start codon compared to variant Tpm3.13. The encoded isoform (Tpm3.5cy, also known as isoform 5) has distinct N- and C- termini and is shorter than isoform Tpm3.13cy. Isoforms Tpm3.1cy, Tpm3.2cy, and Tpm3.5cy are the same length, but contain distinct C-termini.
      Source sequence(s)
      AI842950, AK050665, BB690682, BY284672
      UniProtKB/TrEMBL
      Q8C7C3
      Conserved Domains (1) summary
      pfam00261
      Location:12 → 246
      Tropomyosin

    4. NM_001293748.1 → NP_001280677.2  tropomyosin alpha-3 chain isoform Tpm3.12st

      See identical proteins and their annotated locations for NP_001280677.2

      Status: VALIDATED

      Description
      Transcript Variant: This variant (2, also known as variant Tpm3.12) differs in the 3' UTR and has multiple differences in the 3' coding region compared to variant Tpm3.13. The encoded isoform (Tpm3.12st, also known as isoform 2) is the same length as isoform Tpm3.13cy, but contains a distinct C-terminal segment. The encoded protein is the fast skeletal muscle isoform.
      Source sequence(s)
      AC163616
      UniProtKB/Swiss-Prot
      P21107, Q09021, Q60606, Q80SW6, Q9EPW3
      Related
      ENSMUSP00000113578.2, ENSMUST00000121503.8
      Conserved Domains (2) summary
      pfam00261
      Location:49 → 283
      Tropomyosin
      pfam16526
      Location:14 → 73
      CLZ; C-terminal leucine zipper domain of cyclic nucleotide-gated channels

    5. NM_001293749.1 → NP_001280678.1  tropomyosin alpha-3 chain isoform Tpm3.4cy

      Status: VALIDATED

      Description
      Transcript Variant: This variant (6, also known as variant Tpm3.4) represents the use of an alternate promoter, differs in the 5' and 3' structures and has multiple differences in the coding region compared to variant Tpm3.13. These differences cause translation initiation at an alternate start codon compared to variant Tpm3.13. The encoded isoform (Tpm3.4cy, also known as isoform 6) has distinct N- and C- termini and is shorter than isoform Tpm3.13cy.
      Source sequence(s)
      AC163616, BF464045
      Consensus CDS
      CCDS79961.1
      UniProtKB/TrEMBL
      D3Z6I8
      Related
      ENSMUSP00000113219.2, ENSMUST00000119158.8
      Conserved Domains (1) summary
      pfam00261
      Location:12 → 245
      Tropomyosin; Tropomyosin

    6. NM_001357581.1 → NP_001344510.1  tropomyosin alpha-3 chain isoform Tpm3.3cy

      Status: VALIDATED

      Source sequence(s)
      AC163616, AI842950
      Consensus CDS
      CCDS89659.1
      UniProtKB/TrEMBL
      E9Q7Q3
      Related
      ENSMUSP00000113056.2, ENSMUST00000118566.8
      Conserved Domains (1) summary
      pfam00261
      Location:12 → 246
      Tropomyosin

    7. NM_022314.3 → NP_071709.3  tropomyosin alpha-3 chain isoform Tpm3.13cy

      See identical proteins and their annotated locations for NP_071709.3

      Status: VALIDATED

      Description
      Transcript Variant: This variant (1, also known as variant Tpm3.13) encodes isoform Tpm3.13cy (also known as isoform 1). This isoform is the same length as isoform Tpm3.12st, but contains a distinct C-terminal segment.
      Source sequence(s)
      AK146557, BC029186, BY622941, BY770803
      Consensus CDS
      CCDS38499.2
      UniProtKB/TrEMBL
      E9Q5J9
      Related
      ENSMUSP00000113978.2, ENSMUST00000119570.8
      Conserved Domains (1) summary
      pfam00261
      Location:49 → 283
      Tropomyosin

    RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference GRCm39 C57BL/6J

    Genomic

    1. NC_000069.7 Reference GRCm39 C57BL/6J

      Range
      89979958..90008209
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. XM_006501839.4 → XP_006501902.1  tropomyosin alpha-3 chain isoform X6

      Conserved Domains (1) summary
      pfam00261
      Location:12 → 245
      Tropomyosin

    2. XM_006501835.2 → XP_006501898.1  tropomyosin alpha-3 chain isoform X1

      Conserved Domains (1) summary
      pfam00261
      Location:12 → 246
      Tropomyosin

    3. XM_006501836.2 → XP_006501899.1  tropomyosin alpha-3 chain isoform X2

      Conserved Domains (1) summary
      pfam00261
      Location:12 → 246
      Tropomyosin

    4. XM_017319677.3 → XP_017175166.1  tropomyosin alpha-3 chain isoform X3

      UniProtKB/TrEMBL
      Q8C7C3
      Conserved Domains (1) summary
      pfam00261
      Location:12 → 246
      Tropomyosin

    5. XM_036163205.1 → XP_036019098.1  tropomyosin alpha-3 chain isoform X5

      UniProtKB/TrEMBL
      E9Q7Q3
      Conserved Domains (1) summary
      pfam00261
      Location:12 → 246
      Tropomyosin

    6. XM_030252742.1 → XP_030108602.1  tropomyosin alpha-3 chain isoform X4

      Conserved Domains (1) summary
      pfam00261
      Location:12 → 246
      Tropomyosin

    7. XM_006501841.3 → XP_006501904.1  tropomyosin alpha-3 chain isoform X7

      Conserved Domains (1) summary
      pfam00261
      Location:23 → 180
      Tropomyosin

    RNA

    1. XR_004941375.1 RNA Sequence

    Nucleotide Protein Strain
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    P21107.3 GenPept UniProtKB/Swiss-Prot:P21107

    Gene LinkOut

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