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    1ab replicase ORF1a polyprotein;replicase ORF1ab polyprotein [ Equine arteritis virus ]

    Gene ID: 921339, updated on 6-Oct-2023

    Summary

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    Gene symbol
    1ab
    Gene description
    replicase ORF1a polyprotein;replicase ORF1ab polyprotein
    Locus tag
    EAVgp1
    Gene type
    protein coding
    RefSeq status
    REVIEWED
    Organism
    Equine arteritis virus (strain: Bucyrus)
    Lineage
    Viruses; ssRNA viruses; ssRNA positive-strand viruses, no DNA stage; Nidovirales; Arteriviridae
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    Genomic context

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    Sequence:
    NC_002532.2 (225..9751)

    NC_002532.2Genomic Context describing neighboring genes Neighboring gene glycoprotein 2b (GP2b) Neighboring gene envelope (E) protein Neighboring gene glycoprotein 3 (GP3) Neighboring gene glycoprotein 4 (GP4)

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_002532.2

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Arterivirus nsp12 versus the coronavirus nsp16 2'-O-methyltransferase: comparison of the C-terminal cleavage products of two nidovirus pp1ab polyproteins. Lehmann KC, et al. J Gen Virol, 2015 Sep. PMID 26041874, Free PMC Article
    2. Equine arteritis virus does not induce interferon production in equine endothelial cells: identification of nonstructural protein 1 as a main interferon antagonist. Go YY, et al. Biomed Res Int, 2014. PMID 24967365, Free PMC Article
    3. Arterivirus subgenomic mRNA synthesis and virion biogenesis depend on the multifunctional nsp1 autoprotease. Tijms MA, et al. J Virol, 2007 Oct. PMID 17626105, Free PMC Article
    4. Proteolytic maturation of replicase polyprotein pp1a by the nsp4 main proteinase is essential for equine arteritis virus replication and includes internal cleavage of nsp7. van Aken D, et al. J Gen Virol, 2006 Dec. PMID 17098961
    5. Site-directed mutagenesis of the Nidovirus replicative endoribonuclease NendoU exerts pleiotropic effects on the arterivirus life cycle. Posthuma CC, et al. J Virol, 2006 Feb. PMID 16439522, Free PMC Article

    See all (9) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Nsp12 does not display methyltransferase activity and plays an essential role during EAV replication.
      Title: Arterivirus nsp12 versus the coronavirus nsp16 2'-O-methyltransferase: comparison of the C-terminal cleavage products of two nidovirus pp1ab polyproteins.
    2. Using an IFN-beta promoter-luciferase reporter assay, we subsequently demonstrated that EAV nsps 1, 2, and 11 had the capability to inhibit type I IFN activation.
      Title: Equine arteritis virus does not induce interferon production in equine endothelial cells: identification of nonstructural protein 1 as a main interferon antagonist.
    3. nsp1 is involved in at least three consecutive key processes in the EAV life cycle: replicase polyprotein processing, transcription, and virion biogenesis.
      Title: Arterivirus subgenomic mRNA synthesis and virion biogenesis depend on the multifunctional nsp1 autoprotease.
    4. data suggest that the arterivirus nsp11 is a multifunctional protein with a key role in viral RNA synthesis and additional functions in the viral life cycle that are as yet poorly defined.
      Title: Site-directed mutagenesis of the Nidovirus replicative endoribonuclease NendoU exerts pleiotropic effects on the arterivirus life cycle.
    5. highly regulated, nsp4-mediated processing of the C-terminal half of pp1a is a crucial event in the arterivirus life cycle
      Title: Proteolytic maturation of replicase polyprotein pp1a by the nsp4 main proteinase is essential for equine arteritis virus replication and includes internal cleavage of nsp7.
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    General gene information

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    Miscellaneous features

    • Loc: 5399-5405 ribosomal frameshift slippery sequence

    General protein information

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    Preferred Names
    replicase ORF1a polyprotein;replicase ORF1ab polyprotein
    Names
    nsp1 (PCP1b)
    cysteine proteinase 2
    hydrophobic domain
    3C-like serine proteinase
    hydrophobic domain
    nsp6
    nsp7
    RNA-dependent RNA polymerase
    metal-binding and NTPase/helicase domains
    nsp11
    nsp12
    nsp8
    NP_127506.1
    • ORF1ab
      slippery sequence causes -1 frameshift
    NP_127507.1
    • ORF1a

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_002532.2 Reference assembly

      Range
      225..9751
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NP_127506.1 replicase ORF1ab polyprotein [Equine arteritis virus]

      See identical proteins and their annotated locations for NP_127506.1

      Status: REVIEWED

      UniProtKB/Swiss-Prot
      P19811, Q88625, Q8QZQ5, Q91DM2
      UniProtKB/TrEMBL
      B5LAD9, B5LAE8
      Conserved Domains (11) summary
      pfam05412
      Location:261 → 357
      Peptidase_C33; Equine arterivirus Nsp2-type cysteine proteinase
      pfam05579
      Location:963 → 1269
      Peptidase_S32; Equine arteritis virus serine endopeptidase S32
      pfam14754
      Location:1 → 249
      IFR3_antag; Papain-like auto-proteinase
      pfam12581
      Location:3084 → 3124
      DUF3756; Protein of unknown function (DUF3756)
      cd18786
      Location:2701 → 2760
      SF1_C; C-terminal helicase domain of superfamily 1 DEAD/H-box helicases
      cd17937
      Location:2521 → 2665
      DEXXYc_viral_SF1-N; DEXXY-box helicase domain of viral superfamily 1 helicase
      pfam14755
      Location:361 → 508
      ER-remodelling; Intracellular membrane remodeller
      pfam16749
      Location:1454 → 1573
      Arteri_nsp7a; Arterivirus nonstructural protein 7 alpha
      pfam17873
      Location:2453 → 2505
      Rep_1B; Replicase polyprotein 1ab
      pfam17977
      Location:2371 → 2438
      zf-RING_13; RING/Ubox like zinc-binding domain
      cl02808
      Location:2130 → 2307
      RT_like; Reverse transcriptase (RT, RNA-dependent DNA polymerase)_like family. An RT gene is usually indicative of a mobile element such as a retrotransposon or retrovirus. RTs occur in a variety of mobile elements, including retrotransposons, retroviruses, group ...

    2. NP_705583.1 nsp1 (PCP1b)

      UniProtKB/TrEMBL
      Q7TG98
      Conserved Domains (1) summary
      pfam14754
      Location:1 → 249
      IFR3_antag; Papain-like auto-proteinase

    3. NP_705584.1 cysteine proteinase 2

      Conserved Domains (2) summary
      pfam05412
      Location:1 → 97
      Peptidase_C33; Equine arterivirus Nsp2-type cysteine proteinase
      pfam14755
      Location:101 → 248
      ER-remodelling; Intracellular membrane remodeller

    4. NP_705585.1 hydrophobic domain

      Conserved Domains (1) summary
      pfam05579
      Location:132 → 233
      Peptidase_S32; Equine arteritis virus serine endopeptidase S32

    5. NP_705586.1 3C-like serine proteinase

      Conserved Domains (1) summary
      pfam05579
      Location:1 → 204
      Peptidase_S32; Equine arteritis virus serine endopeptidase S32

    6. NP_705587.1 hydrophobic domain

    7. NP_705588.1 nsp6

    8. NP_705589.1 nsp7

      Conserved Domains (1) summary
      pfam16749
      Location:2 → 123
      Arteri_nsp7a; Arterivirus nonstructural protein 7 alpha

    9. NP_705590.1 RNA-dependent RNA polymerase

      Conserved Domains (1) summary
      cl02808
      Location:453 → 630
      RT_like; RT_like: Reverse transcriptase (RT, RNA-dependent DNA polymerase)_like family. An RT gene is usually indicative of a mobile element such as a retrotransposon or retrovirus. RTs occur in a variety of mobile elements, including retrotransposons, ...

    10. NP_705591.1 metal-binding and NTPase/helicase domains

      Conserved Domains (1) summary
      pfam01443
      Location:156 → 390
      Viral_helicase1; Viral (Superfamily 1) RNA helicase

    11. NP_705592.1 nsp11

    12. NP_705593.1 nsp12

      Conserved Domains (1) summary
      pfam12581
      Location:28 → 68
      DUF3756; Protein of unknown function (DUF3756)

    13. NP_705594.1 nsp8

    14. NP_127507.1 replicase ORF1a polyprotein [Equine arteritis virus]

      See identical proteins and their annotated locations for NP_127507.1

      Status: REVIEWED

      UniProtKB/TrEMBL
      B5LAE0, B5LAE9, B5LAF8
      Conserved Domains (5) summary
      pfam05412
      Location:261 → 357
      Peptidase_C33; Equine arterivirus Nsp2-type cysteine proteinase
      pfam05579
      Location:963 → 1269
      Peptidase_S32; Equine arteritis virus serine endopeptidase S32
      pfam14755
      Location:361 → 508
      ER-remodelling; Intracellular membrane remodeller
      pfam14754
      Location:1 → 249
      IFR3_antag; Papain-like auto-proteinase
      pfam16749
      Location:1454 → 1575
      Arteri_nsp7a; Arterivirus nonstructural protein 7 alpha
    Nucleotide Protein
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