Lassa fever virus is a Category A zoonotic pathogen that infects rodents and is occasionally transmitted to humans with fatal results. Following a pilot experiment to establish the optimal library construction method, we propose to sequence 26 viral isolates derived from passages of an attenuated re-assorted Lassa vaccine candidate strain (Mopeia-Lassa
More...29 or ML29) in mice and non-human primates. This will allow the in vivo variation of this vaccine strain to be assessed. Lassa virus has a bi-segmented, single-stranded RNA genome; the ML29 vaccine candidate is a re-assorted virus containing the large (L) genomic segment from the non-pathogenic Mopeia virus and the small (S) segment from Lassa virus. The large genomic segment is 7.2 kb, and the small segment is 3.4 kb, for a total genome size of 10.5kb. Less...| Sequence data: | genome assemblies: 2 | | | | Statistics: | median total length (Mb): 0.010689 | | median protein count: 4 | | median GC%: 42.1427 |
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