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| Status |
Public on Nov 08, 2017 |
| Title |
To compare the transcriptome of ompR null strains with wild-type strain of S. Typhimurium during osmotic stress |
| Organism |
Salmonella enterica subsp. enterica serovar Typhimurium str. 14028S |
| Experiment type |
Expression profiling by array
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| Summary |
In bacteria, one paradigm for signal transduction is the two-component regulatory system, consisting of a sensor kinase (usually a membrane protein) and a response regulator (usually a membrane protein) and a response regulator (usually a DNA binding protein). The EnvZ/OmpR two-component system in S. Typhimurium responds to osmotic stress and regulates expression of outer membrane proteins. Furthermore, bacteria were believed to regulate pH by acidifying after external acid stress, then immediately recovering. Using a pH-sensor in single living cells, we show that S. Typhimurium maintain an acidic cytoplasm under sucrose-driven osmotic stress which requires OmpR. Thus, we set out to identify the OmpR targets of S. Typhimurium under osmotic stress.
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| Overall design |
To identify OmpR targets under osmotic stress conditions, we performed a microarray and compared the transcriptome of ompR null strains with wild-type strains of S. Typhimurium at high osmolality. S. Typhimurium strain was grown in MgM media at 7.2 and 7.2 with 15% (w/v) sucrose to O.D ~0.6. The total RNA was isolated using an RNeasy mini kit (Qiagen) and proceeded for Microarray
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| Contributor(s) |
Chakraborty S, Kenney LJ |
| Citation(s) |
29138484, 30524381 |
| |
| Submission date |
Nov 07, 2017 |
| Last update date |
Apr 16, 2021 |
| Contact name |
smarajit chakraborty |
| E-mail(s) |
chakrabortysmarajit@gmail.com
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| Phone |
83682977
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| Organization name |
Mechanobiology Institute
|
| Street address |
T-Lab, 5A Engineering Drive 1, NUS
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| City |
singapore |
| ZIP/Postal code |
117411 |
| Country |
Singapore |
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| Platforms (1) |
| GPL24239 |
Agilent-048242 Salmonella_Enterica_str14028S [Probe Name Version] |
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| Samples (8)
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| GSM2844079 |
S. Typhimurium_WT pH 7.2+15% sucrose D |
| GSM2844080 |
S. Typhimurium_∆ompR pH 7.2+15% sucrose A |
| GSM2844081 |
S. Typhimurium_∆ompR pH 7.2+15% sucrose B |
| GSM2844082 |
S. Typhimurium_∆ompR pH 7.2+15% sucrose C |
| GSM2844083 |
S. Typhimurium_∆ompR pH 7.2+15% sucrose D |
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| This SubSeries is part of SuperSeries: |
| GSE106630 |
To compare the transcriptome of ompR null strains with wild-type strain of E. coli and S. Typhimurium during stress |
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| Relations |
| BioProject |
PRJNA417483 |
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