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Series GSE107624 Query DataSets for GSE107624
Status Public on Dec 01, 2018
Title Global Transcriptome Analysis during Mucociliary Differentiation of Human Nasal Polyp/Nasal Mucosa Basal Stem/Progenitor Cells
Platform organisms Homo sapiens; synthetic construct
Sample organism Homo sapiens
Experiment type Expression profiling by array
Non-coding RNA profiling by array
Summary The aim of this study was to perform a genome-wide transcriptional analysis (mRNA + microRNA) during in vitro mucociliary differentiation of primary human basal stem/progenitors cells (BSCs) cultured at the air-liquid interface (ALI) system.
We used microarrays to detail the global gene expression underlying mucociliary differentiation of human upper airways basal stem/progenitor cells isolated from nasal polyps and control nasal mucosa.
 
Overall design Basal stem/progenitor cells were isolated from Nasal Polyps or control Nasal Mucosa by the explant technique, cultured and differentiated at the air-liquid interface (ALI) cell culture system. At the different days of ALI culture (0, 14 and 28 days), total RNA was extracted from cells and finally processed for microarrays analysis (Human Genome U219 24-Array Plate).
 
Contributor(s) Callejas-Díaz B, Fernàndez G, Mullol J
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Submission date Dec 02, 2017
Last update date Mar 21, 2019
Contact name Borja Callejas-Díaz
Organization name IDIBAPS, HOSPITAL CLÍNIC
Department Clinical and Experimental Respiratory Immunoallergy
Lab IRCE Lab
Street address Carrer de Casanova, 143, CELLEX Sector 2B
City BARCELONA
State/province CATALUÑA
ZIP/Postal code 08036
Country Spain
 
Platforms (2)
GPL13667 [HG-U219] Affymetrix Human Genome U219 Array
GPL19117 [miRNA-4] Affymetrix Multispecies miRNA-4 Array
Samples (84)
GSM2872752 Nasal Mucosa ALI at day 0, biological rep 1 (mRNA)
GSM2872753 Nasal Mucosa ALI at day 0, biological rep 2 (mRNA)
GSM2872754 Nasal Mucosa ALI at day 0, biological rep 3 (mRNA)
Relations
BioProject PRJNA420859

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE107624_RAW.tar 144.5 Mb (http)(custom) TAR (of CEL, CHP)
Processed data included within Sample table
Processed data provided as supplementary file
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