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| Status |
Public on Jun 01, 2008 |
| Title |
Rapid encoding of information alters the profile of plasticity related mRNA transcripts in the hippocampal DG region. |
| Organism |
Rattus norvegicus |
| Experiment type |
Expression profiling by array
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| Summary |
A theoretical framework for the function of the medial temporal lobe system in memory defines differential contributions of the hippocampal subregions with regard to pattern recognition retrieval processes and encoding of new information. To investigate molecular programs of relevance, we designed a spatial learning protocol to engage a pattern separation function to encode new information. After background training, two groups of animals experienced the same new training in a novel environment, however only one group was provided spatial information and demonstrated spatial memory in a retention test. Global transcriptional analysis of the microdissected subregions of the hippocampus exposed a CA3 pattern that was sufficient to clearly segregate spatial learning animals from control. Individual gene and functional group analysis anchored these results to previous work in neural plasticity. From a multitude of expression changes, increases in camk2a, rasgrp1 and nlgn1 were confirmed by in situ hybridization. Furthermore, siRNA inhibition of nlgn1 within the CA3 subregion impaired spatial memory performance, pointing to mechanisms of synaptic remodeling as a basis for rapid encoding of new information in long-term memory.
Keywords: behavioral intervention: spatial learning paradigm
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| Overall design |
RNA samples from animals subjected to a spatial learning paradigm were compared to controls using Affymetirx RAE230a chips. An N of 7 was used in each of the two experimental conditions.
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| Web link |
http://nbc.jhu.edu/la/
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| Contributor(s) |
Haberman RP, Lee HJ, Colantuoni C, Koh MT, Gallagher M |
| Citation(s) |
18650386 |
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| Submission date |
May 16, 2008 |
| Last update date |
Mar 03, 2017 |
| Contact name |
Carlo Colantuoni |
| E-mail(s) |
ccolantu@jhmi.edu
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| Phone |
4104931439
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| Organization name |
Johns Hopkins Univ. School of Medicine
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| Department |
Neurology
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| Street address |
733 N Broadway
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| City |
Baltimore |
| State/province |
MD |
| ZIP/Postal code |
21205 |
| Country |
USA |
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| Platforms (1) |
| GPL341 |
[RAE230A] Affymetrix Rat Expression 230A Array |
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| Samples (14)
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| GSM289087 |
Control DG, biological rep4 |
| GSM289088 |
Control DG, biological rep5 |
| GSM289089 |
Control DG, biological rep6 |
| GSM289090 |
Control DG, biological rep7 |
| GSM289091 |
Learning Activated DG, biological rep1 |
| GSM289092 |
Learning Activated DG, biological rep2 |
| GSM289093 |
Learning Activated DG, biological rep3 |
| GSM289094 |
Learning Activated DG, biological rep4 |
| GSM289095 |
Learning Activated DG, biological rep5 |
| GSM289096 |
Learning Activated DG, biological rep6 |
| GSM289097 |
Learning Activated DG, biological rep7 |
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| This SubSeries is part of SuperSeries: |
| GSE11476 |
Rapid encoding of new information alters the profile of plasticity related mRNA transcripts in the hippocampal region |
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| Relations |
| BioProject |
PRJNA109123 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE11475_RAW.tar |
22.9 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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