|
| Status |
Public on Apr 22, 2020 |
| Title |
miRNA profiling of cultured primary recessive dystrophic epidermolysis bullosa (RDEB) squamous cell carcinoma (SCC) |
| Platform organism |
synthetic construct |
| Sample organism |
Homo sapiens |
| Experiment type |
Non-coding RNA profiling by array
|
| Summary |
Recessive dystrophic epidermolysis bullosa (RDEB) is a monogenetic skin disorder caused by mutations in the COL7A1 gene. Missing type VII collagen leads to severe blister formation and frequent chronic wounds. Patients suffering from RDEB are prone to develop particulary aggressive squamous cell carcinoma (SCC), representing the major cause of mortality. This dataset provides Affymetrix microarray (miRNA4.1) based whole transcriptome data on RNA isolated from cultured primary keratinocytes (KC) as well as squamous cell carcinoma (SCC).
Cells were derived from punch biopsies or tumor resections from either healthy donors or SCC patients with or without the diagnosis recessive dystrophic epidermolysis bullosa (RDEB). Primary KC and SCC were cultivated in fully defined medium till subconfluency. Total RNA was isolated and microarray assay performed.
|
| |
|
| Overall design |
A case-control study design of cultured primary cells representing RDEB-SCC, RDEB-KC, non-EB SCC, healthy control KC.
|
| |
|
| Contributor(s) |
Wimmer M, Zauner R, Wally V |
| Citation(s) |
32276641 |
| |
| Submission date |
May 06, 2019 |
| Last update date |
Apr 30, 2021 |
| Contact name |
Roland Zauner |
| E-mail(s) |
rolan.zauner@salk.at
|
| Organization name |
EB House Austria
|
| Department |
University Hospital of Dermatology Salzburger Landeskliniken (SALK) | Paracelsus Medical University Salzburg (PMU)
|
| Lab |
small molecules & epigenetics
|
| Street address |
Muellner Hauptstrasse 48
|
| City |
Salzburg |
| State/province |
Salzburg |
| ZIP/Postal code |
5020 |
| Country |
Austria |
| |
|
| Platforms (1) |
| GPL19117 |
[miRNA-4] Affymetrix Multispecies miRNA-4 Array |
|
| Samples (20)
|
|
| Relations |
| BioProject |
PRJNA541300 |
Less...
More...