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| Status |
Public on Sep 28, 2022 |
| Title |
mRNA-sequencing of ILC1 cells |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Purpose: To understanding the effects of RORa deficiency on ILC1 cells, we conducted bulk mRNA-seqencing
Method: Firstly, we purified liver ILC1 cells (CD45.2+CD3-CD19-NKp46+NK1.1+CD49a+CD49b-) via Fluorescence Activated Cell Sorting, then frozen in -80 °C ultra-low temperature refrigerator, followed by High-throughput sequencing, in three replicates for WT (Rorafl/fl) mice and one replicate for cKO (Ncr1Cre-Rorafl/fl) , using Illumina Hiseq 1500 platform.
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| Overall design |
Find the DEGs in cKO ILC1 cells
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| |
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| Contributor(s) |
Song J, Song H, Sun R, Tian Z, Peng H |
| Citation(s) |
34510508 |
| |
| Submission date |
Oct 20, 2021 |
| Last update date |
Sep 28, 2022 |
| Contact name |
Zhigang Tian |
| E-mail(s) |
tzg@ustc.edu.cn
|
| Phone |
+8615665425083
|
| Organization name |
Univerity of Science and Technology of China
|
| Street address |
Huangshan Road，number 443
|
| City |
Hefei |
| State/province |
Anhui |
| ZIP/Postal code |
230000 |
| Country |
China |
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| Platforms (1) |
| GPL18480 |
Illumina HiSeq 1500 (Mus musculus) |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA772937 |
| SRA |
SRP342256 |
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