|
| Status |
Public on Feb 01, 2024 |
| Title |
CircAkt3 participates in bone cancer pain progression in rats by modulating MAPK signalling pathway |
| Organism |
Rattus norvegicus |
| Experiment type |
Expression profiling by high throughput sequencing
Non-coding RNA profiling by high throughput sequencing
|
| Summary |
CircRNA parent genes were mainly enriched in MAPK and neurodevelopmental signalling pathways. CircAkt3 and circMap4k1 were significantly up-regulated in the BCP group. CircaAkt3 may increase p-ERK expression by upregulating Col8a1, which may further activate the MAPK pathway.
|
| |
|
| Overall design |
We established a BCP rat model. The top four differentially expressed circRNAs (DECs) in the model were validated by agarose gel electrophoresis and Sanger sequencing between the BCP group and sham group. A circRNA-miRNA-mRNA network was constructed based on the interactions among circRNAs, microRNA (miRNA), and mRNA, which were predicted by TargetScan. mRNA and circRNA expression levels were detected by quantitative RT-PCR. In addition, Western Blot was performed to identify the protein levels of p-ERK, ERK, and Col8a1.
|
| |
|
| Contributor(s) |
Chen L, Zhang X, Su Y, Zhang Y |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Feb 02, 2023 |
| Last update date |
Feb 01, 2024 |
| Contact name |
chen wei fen |
| E-mail(s) |
chenwf0927@gmail.com
|
| Phone |
13713896659
|
| Organization name |
Forevergen
|
| Street address |
4F, Building 1, Standard Property Unit 3, International Biotech Island
|
| City |
Guangzhou |
| ZIP/Postal code |
510300 |
| Country |
China |
| |
|
| Platforms (1) |
| GPL25947 |
Illumina NovaSeq 6000 (Rattus norvegicus) |
|
| Samples (2) |
|
| Relations |
| BioProject |
PRJNA930768 |
