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| Status |
Public on Nov 02, 2011 |
| Title |
Study for evaluating the effect of cold ischemic time and RNA stabilization method on RNA integrity and gene expression measurements |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Time series of eleven breast cancer samples subjected to different cold ischemic stress of up to 3 hr post tumor excision.
A different 2x2 factorial within this study evaluated the effect of stabilization method (RNAlater vs snap freezing) and stablization delay (0 and 40 min) at room temperature.
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| Overall design |
Tissue samples were collected at surgery, cut into 1-2 mm pieces and divided into 8 portions. Portions were put in RNAlater or fresh frozen at baseline, 20, 40, 60, 120, and 180 minutes thereafter, or snap frozen in dry ice in a pre-chilled sample vial at baseline and 40 minutes thereafter.
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| Contributor(s) |
Symmans WF, Sun H, Hatzis C |
| Citation(s) |
22034635, 24602449 |
| |
| Submission date |
Oct 29, 2010 |
| Last update date |
Feb 03, 2020 |
| Contact name |
Christos Hatzis |
| E-mail(s) |
christos@nuverabio.com
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| Phone |
781-938-3830
|
| URL |
http://www.nuverabio.com
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| Organization name |
Nuvera Biosciences
|
| Street address |
400 West Cummings Park, Suite 5350
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| City |
Woburn |
| State/province |
MA |
| ZIP/Postal code |
01801 |
| Country |
USA |
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| Platforms (1) |
| GPL96 |
[HG-U133A] Affymetrix Human Genome U133A Array |
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| Samples (86)
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| Relations |
| BioProject |
PRJNA134373 |
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