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| Status |
Public on Mar 18, 2011 |
| Title |
Microarray gene expression profiling of heart failure induced in apolipoprotein E-deficient mice by treatment with rosiglitazone |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
The anti-diabetic drug and agonist of the peroxisome proliferator-activated receptor gamma (Pparg), rosiglitazone, was recently withdrawn in many countries because the drug use was associated with an increased risk of heart failure. To investigate underlying pathomechanisms, we chose 6-month-old apolipoprotein E (apoE)-deficient mice, which are prone to atherosclerosis and insulin resistance, and thereby mimic the risk profile of patients with cardiovascular disease. After 8 weeks of rosiglitazone treatment (30 mg/kg/day), echocardiography and histology analyses demonstrated that rosiglitazone had induced heart failure with cardiac dilation. Concomitantly, cardiac lipid overload and lipid-induced cardiomyocyte death developed. The microarray gene expression study of heart tissue from rosiglitazone-treated apoE-deficient mice relative to untreated apoE-deficient mice and non-transgenic B6 mice identified cardiac Pparg-dependent lipid metabolism genes in rosiglitazone-treated mice, which seem to trigger a major heart failure promoting pathway.
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| Overall design |
Microarray gene expression profiling was performed with heart tissue isolated from three study groups: (i) rosiglitazone-treated 8-month-old apolipoprotein (apoE)-deficient mice with symptoms of heart failure, (ii) untreated 8-month-old apoE-deficient mice, and (iii) age-matched, untreated, non-transgenic B6 control mice.
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| Contributor(s) |
Abd Alla J, Quitterer U |
| Citation(s) |
26670611 |
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| Submission date |
Mar 17, 2011 |
| Last update date |
Feb 11, 2019 |
| Contact name |
Ursula Quitterer |
| E-mail(s) |
ursula.quitterer@pharma.ethz.ch
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| Phone |
+41-446329801
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| Organization name |
ETH Zurich
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| Department |
Molecular Pharmacology
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| Street address |
Winterthurerstrasse 190
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| City |
Zurich |
| ZIP/Postal code |
CH-8057 |
| Country |
Switzerland |
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| Platforms (1) |
| GPL1261 |
[Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array |
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| Samples (6)
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| GSM693652 |
Failing heart_apoE-deficient_2 months of rosiglitazone_age 8 months_rep1 |
| GSM693653 |
Failing heart_apoE-deficient_2 months of rosiglitazone_age 8 months_rep2 |
| GSM693654 |
Heart_apoE-deficient_age 8 months_rep1 |
| GSM693655 |
Heart_apoE-deficient_age 8 months_rep2 |
| GSM693656 |
Control heart_age 8 months_BSIX_rep1 |
| GSM693657 |
Control heart_age 8 months_BSIX_rep2 |
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| Relations |
| BioProject |
PRJNA137955 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE28031_RAW.tar |
24.7 Mb |
(http)(custom) |
TAR (of CEL, CHP) |
| Processed data included within Sample table |
| Processed data provided as supplementary file |
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