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| Status |
Public on Oct 31, 2012 |
| Title |
Human annulus disc cells grown in a 3D collagen sponge and exposed to TNF-a |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
The objective of this study was to determine how TNF-a, an important proinflammatory cytokine, affects gene expression in the human annulus.
Cells were grown in a 3D collagen construct for 14 days with TNF-a. mRNA was isolated and subjected to microarray. Fold changes in gene expression were determined via GeneSifer software.
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| Overall design |
Human disc tissue samples were obtained from surgical disc procedures performed on patients with herniated discs and degenerative disc disease. Cultured annulus cells were grown in a 3D collagen construct with or without 10e3 pM TNF-a for a total of 14 days. Following homogenization in TRIzol reagent, total RNA was isolated and analyzed via mircoarray.
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| Contributor(s) |
Gruber HE, Hoelscher GL, Ingram JA, Hanley EN Jr |
| Citation(s) |
24468005 |
| |
| Submission date |
Oct 28, 2012 |
| Last update date |
Nov 02, 2023 |
| Contact name |
Helen Gruber |
| E-mail(s) |
Helen.Gruber@carolinashealthcare.org
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| Organization name |
Carolinas HealthCare System
|
| Department |
Orthopaedic Surgery
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| Lab |
Orthopaedic Research
|
| Street address |
1542 Graden Terrace
|
| City |
Charlotte |
| State/province |
NC |
| ZIP/Postal code |
28203 |
| Country |
USA |
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| Platforms (1) |
| GPL1352 |
[U133_X3P] Affymetrix Human X3P Array |
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| Samples (8)
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| Relations |
| BioProject |
PRJNA178513 |
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