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| Status |
Public on Jun 12, 2013 |
| Title |
Gene expression changes of Saccharomyces cerevisiae to linoleic acid hydroperoxide |
| Platform organisms |
Schizosaccharomyces pombe; Saccharomyces cerevisiae |
| Sample organism |
Saccharomyces cerevisiae |
| Experiment type |
Expression profiling by array
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| Summary |
Reactive oxygen species, generated in vivo or exogenously encountered, constantly challenge living organisms. Oxidation of polyunsaturated fatty acids (PUFA), which are susceptible to oxidant attack, can lead to initiation of lipid peroxidation and in turn rapid production of toxic lipid hydroperoxides. Eukaryotic microorganisms such as Saccharomyces cerevisiae can survive harsh industrial conditions that contain high levels of the PUFA linoleic acid and its oxidised derivative, linoleic acid hydroperoxide (LoaOOH). The precise signalling and response mechanisms induced by yeast to overcome lipid hydroperoxide stress are ill understood.
We used genome-wide microarrays to investigate the changes in gene expression of S. cerevisiae to LoaOOH-induced oxidative stress.
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| Overall design |
S. cerevisiae (BY4743) were exposed to an arresting concentration of LoaOOH (75 µM) for 1 hr to induce oxidative stress. Yeast treated with an equivalent volume of solvent (methanol) were used as a control. Following treatment conditions, total RNA was extracted from LoaOOH-treated or control yeast and hybridised onto Affymetrix microarrays.
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| Contributor(s) |
O’Doherty PJ, Lyons V, Higgins VJ, Rogers PJ, Bailey TD, Wu MJ |
| Citation(s) |
24074273 |
| |
| Submission date |
Jun 11, 2013 |
| Last update date |
Feb 21, 2017 |
| Contact name |
Patrick John O'Doherty |
| Organization name |
University of Western Sydney
|
| Street address |
Goldsmith Ave
|
| City |
Sydney |
| State/province |
NSW |
| ZIP/Postal code |
2560 |
| Country |
Australia |
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| Platforms (1) |
| GPL2529 |
[Yeast_2] Affymetrix Yeast Genome 2.0 Array |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA208018 |
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