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Series GSE54070 Query DataSets for GSE54070
Status Public on Jan 16, 2014
Title Detection of antibiotic resistance genes in human gut microbiota
Platform organism gut metagenome
Sample organism human gut metagenome
Experiment type Genome variation profiling by array
Summary We used a DNA microarray chip covering 369 resistance types to investigate the relation of antibiotic resistance gene diversity with humans’ age. Metagenomic DNA from fecal samples of 123 healthy volunteers of four different age groups, i.e. pre-school Children (CH), School Children (SC), High School Students (HSS) and Adults (AD) were used for hybridization. The results showed that 80 different gene types were recovered from the 123 individuals gut microbiota, among which 25 were present in CH, 37 in SC, 58 in HSS and 72 in AD. Further analysis indicated that antibiotic resistance genes in groups of CH, SC and AD can be independently clustered, and those ones in group HSS are more divergent.
The detailed analysis of antibiotic resistance genes in human gut is further described in the paper DNA microarray analysis reveals the antibiotic resistance gene diversity in human gut microbiota is age-related submitted to Sentific Reports
 
Overall design The antibiotic resistance gene microarray is custom-designed (Roche NimbleGen), based on a single chip containing 3 internal replicated probe sets of 12 probes per resistance gene, covering the whole 315K 12-plex platform spots.
 
Contributor(s) Lu N, Hu Y
Citation(s) 24618772
Submission date Jan 14, 2014
Last update date Aug 22, 2019
Contact name Yongfei Hu
E-mail(s) huyf@im.ac.cn
Organization name Institute of Microbiology, Chinese Academy of Sciences
Department CAS key Laboratory of Pathogenic Microbiology and Immunology
Street address NO.1 West Beichen Road, Chaoyang District
City Beijing
ZIP/Postal code 100101
Country China
 
Platforms (1)
GPL18168 NimbleGen gut microbiome array [091124_micro_BZ_EXP_HX12]
Samples (123)
GSM1306672 Human_intestinal_Metagenome_Drug-resistance_Gene_Study_53
GSM1306673 Human_intestinal_Metagenome_Drug-resistance_Gene_Study_54
GSM1306674 Human_intestinal_Metagenome_Drug-resistance_Gene_Study_55
Relations
BioProject PRJNA235167

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE54070_RAW.tar 244.7 Mb (http)(custom) TAR (of PAIR)
Processed data included within Sample table
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