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| Status |
Public on Oct 03, 2006 |
| Title |
hES cell FGF2 re-stimulation experiments |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
This series includes hES cell samples starved and re-stimulated with FGF2. Controls were FGF2-starved but not re-stimulated. Two independent experiments were carried out (one sample plus control each).
Keywords: Growth factor stimulation experiment
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| Overall design |
hES cells were grown in conditioned medium for 2 days, starved in unconditioned medium (UM) without FGF2 and with 30uM of SU5402 (Calbiochem), for 24h, and, after extensive washing, re-treated with 40 ng/ml of FGF2 in UM for 4h. Controls were FGF2-starved but not re-stimulated. After the FGF2 starvation period the cells did not show any obvious signs of spontaneous differentiation.
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| Contributor(s) |
Greber B, Lehrach H, Adjaye J |
| Citation(s) |
17038665 |
| |
| Submission date |
Jul 31, 2006 |
| Last update date |
Mar 16, 2012 |
| Contact name |
Boris Greber |
| E-mail(s) |
boris.greber@mpi-muenster.mpg.de
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| Organization name |
Max Planck Institute for Molecular Biomedicine
|
| Department |
Cell and Developmental Biology
|
| Street address |
Röntgenstraße 20
|
| City |
Münster |
| ZIP/Postal code |
48149 |
| Country |
Germany |
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| Platforms (1) |
| GPL2700 |
Sentrix HumanRef-8 Expression BeadChip |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA96233 |
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