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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Oct 03, 2014 |
| Title |
Expression data comparing Rb/p53 and p53 deficient mammary tumors and normal mammary tissue |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
To investigate the impact of combined Rb and p53 loss in mammary tumorigenesis, we used transgenic and viral approaches to delete Rb and p53 floxed alleles specifically in the mouse mammary epithelium. Although MMTV-Cre (NLST) targets stem/bi-potent progenitors in the mammary gland, a subset of MMTV-Cre:Rbf/f;p53f/f mice developed non-mammary tumors. Thus, freshly isolated primary mammary epithelial cells from these animals were transplanted into the mammary fat pads of immunodeficient mice and monitored for tumor formation. In addition, primary MECs were isolated from Cre-negative Rbf/f;p53f/f mice, infected with Ad-Cre followed by orthotopic transplantation. In all these cases, resulting tumors shared similar spindle-shape histology, expressed high levels of vimentin, a mesenchymal marker, but not E-cadherin, a luminal marker, and were classified as adeno-sacrcomatoid/spindle-cell/mesenchymal-like breast cancer. We used microarrays to detect differentially expressed genes in the Rb/p53 double-knock-out vs p53 single deletion or normal mammary tissue.
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| Overall design |
Total RNA was extracted from tumors developed by double Trizol method and hybridized on Affymetrix microarrays
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| Contributor(s) |
Jones R, Liu J, Zacksenhaus E |
| Citation(s) |
27571409 |
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| Submission date |
Oct 02, 2014 |
| Last update date |
Mar 04, 2019 |
| Contact name |
Robert Jones |
| E-mail(s) |
rjones12@uoguelph.ca
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| Organization name |
University of Guelph
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| Department |
Biomedical Sciences
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| Street address |
50 Stone Rd
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| City |
Guelph |
| ZIP/Postal code |
N1G 2W1 |
| Country |
Canada |
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| Platforms (1) |
| GPL6246 |
[MoGene-1_0-st] Affymetrix Mouse Gene 1.0 ST Array [transcript (gene) version] |
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| Samples (18)
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| Relations |
| BioProject |
PRJNA262942 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE62016_RAW.tar |
81.0 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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